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| Status |
Public on Feb 23, 2021 |
| Title |
Genome-wide DNA-binding analysis for Ttk in intestinal progenitor cells and Su(H) in wildtype, seq overexpressed and ttk-RNAi intestinal progenitor cells by DamID |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Purpose: Genome-wide DNA-binding analysis for Ttk in intestinal progenitor cells and comparing Su(H) binding sites between seq overexpressed and ttk-RNAi intestinal progenitor cells in Drosophila midgut by DNA adenine methyltransferase identification(DamID)
Methods: Dam(Ctrl) , Ttk-Dam transgenes, Su(H)-Dam, Su(H)-Dam&seq and Su(H)-Dam&ttk-RNAi were expressed in intestinal progenitor cells by esg-Gal4.The guts within 2 days was used for DNA adenine methyltransferase identification (DamID), followed by deep sequencing analysis
Results: Ttk suppresses neural specific Notch targets through suppressing Seq in intestinal progenitor cells
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| Overall design |
Dam(Ctrl) , Ttk-Dam transgenes, Su(H)-Dam, Su(H)-Dam&seq and Su(H)-Dam&ttk-RNAi were expressed in intestinal progenitor cells by esg-Gal4.The guts within 2 days was used for DamID-seq, followed by deep sequencing analysis.
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| |
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| Contributor(s) |
Li Z, Xi R |
| Citation(s) |
32049006, 36316343 |
| |
| Submission date |
May 09, 2019 |
| Last update date |
Nov 17, 2022 |
| Contact name |
huanwei huang |
| E-mail(s) |
hhw2436@gmail.com
|
| Organization name |
NIBS
|
| Street address |
7 Science Park Rd., ZGC life science park
|
| City |
Beijing |
| ZIP/Postal code |
102206 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
|
| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE130943 |
Tramtrack safeguards tissue identity of Drosophila intestinal stem cells by preventing neuroblast-like program activation and neuroendocrine tumors |
|
| Relations |
| BioProject |
PRJNA542068 |
| SRA |
SRP197254 |
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