|
| Status |
Public on Feb 23, 2021 |
| Title |
ttk-RNAi-Su(H)-TA-Dam-rep3 |
| Sample type |
SRA |
| |
|
| Source name |
midgut
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: midgut
age: adult stage day 2
genotype: ttk-RNAi and Su(H) overexpression
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Drosophila DamID was carried out according to iDamIDseq based on a method from Gutierrez-Triana JA et al.2016
Libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Library strategy: DamID-seq
Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to BDGP6 genome using Bowtie v1.1.2 with -v 3 -m 1 parameters.
Peaks were called using MACS version 1.4.2 with default parameters.
Genome_build: Ensemble Drosophila melanogaster BDGP6.
Supplementary_files_format_and_content: bigwig files were generated using Homer package.
|
| |
|
| Submission date |
May 09, 2019 |
| Last update date |
Feb 23, 2021 |
| Contact name |
huanwei huang |
| E-mail(s) |
hhw2436@gmail.com
|
| Organization name |
NIBS
|
| Street address |
7 Science Park Rd., ZGC life science park
|
| City |
Beijing |
| ZIP/Postal code |
102206 |
| Country |
China |
| |
|
| Platform ID |
GPL17275 |
| Series (2) |
| GSE130941 |
Genome-wide DNA-binding analysis for Ttk in intestinal progenitor cells and Su(H) in wildtype, seq overexpressed and ttk-RNAi intestinal progenitor cells by DamID |
| GSE130943 |
Tramtrack safeguards tissue identity of Drosophila intestinal stem cells by preventing neuroblast-like program activation and neuroendocrine tumors |
|
| Relations |
| BioSample |
SAMN11607895 |
| SRA |
SRX5809556 |
