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| Status |
Public on Apr 11, 2022 |
| Title |
Induced expression of PD-L1 in spermatogonial stem cells by self-renewal division allows allogeneic offspring production [microarray] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Testis immune privilege is thought to be mediated by somatic cells. However, it is not strong enough to protect allogeneic spermatogonial stem cells (SSCs) transplanted into the seminiferous tubules. Here we report successful production of allogeneic offspring by inducing PD-L1 in SSCs. Activation of self-renewal division induced PD-L1 and B7-H3 expression in cultured SSCs, which produced sperm after allogeneic transplantation. While B7-H3 depletion did not influence colonization, PD-L1 depletion prevented donor-derived spermatogenesis. PD-L1 expression was induced by BCL6B via reactive oxygen species (ROS) generation, suggesting that self-renewal stimulation confers immune privilege by ROS. In contrast, reduced ROS or Mapk14 deficiency downregulated PD-L1 expression. Allogeneic offspring were produced by SSC transplantation into congenitally infertile mice and busulfan-treated wild-type mice. Therefore, SSCs can escape rejection when their self-renewal division is stimulated.
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| Overall design |
Analysis of expression level change during establishment (0weeks, 1weeks, 2weeks, 3weeks, established cultured SSCs) of cultured spermatogonial stem cells
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| Contributor(s) |
Watanabe S, Kanatsu-Shinohara M, Yamamoto T, Shinohara T |
| Citation(s) |
36963391 |
| |
| Submission date |
Apr 09, 2019 |
| Last update date |
May 12, 2023 |
| Contact name |
Satoshi Watanabe |
| E-mail(s) |
watanabe.satoshi.7r@kyoto-u.ac.jp
|
| Phone |
81757514160
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| Organization name |
Kyoto University
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| Department |
Graduate School of Medicine
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| Lab |
Department of Molecular Genetics
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| Street address |
53, Kawahara-cho, Shogoin, Sakyo
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| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE129659 |
Induced expression of PD-L1 in spermatogonial stem cells by self-renewal division allows allogeneic offspring production |
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| Relations |
| BioProject |
PRJNA531697 |
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