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Status |
Public on Apr 11, 2022 |
Title |
GS cells-2 |
Sample type |
RNA |
|
|
Source name |
Already established cutured SSCs (GS cells)
|
Organism |
Mus musculus |
Characteristics |
cell type: spermatogonial stem cells (SSCs)
|
Treatment protocol |
adult DBA/2 testicular cells purified by magnetically sorted using anti-CD9 antibody
|
Growth protocol |
DBA/2 GS cells and adult DBA/2 testicular cells purified by magnetically sorted using anti-CD9 antibody were cultured in culture medium based on Iscove’s modified MEM (Invitrogen, Carlsbad, CA), which was supplemented with 10 ng/ml human FGF2, 15 ng/ml recombinant rat GDNF (both from Peprotech, London, UK) and 1% fetal bovine serum (FBS) (Kanatsu-Shinohara et al, 2014).
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA were extracted by TRIzol (Qiagen) and purified by RNeasy mini kit (Qiagen)
|
Label |
biotin
|
Label protocol |
GeneChip WT Terminal Labeling Kit (Affymetrix) were used according to Affymetrix protocol.
|
|
|
Hybridization protocol |
Fragmented and labelled cDNA was hybridized to GeneChip Mouse Gene 1.0 ST (Affymetrix).
|
Scan protocol |
Arrays were scanned using the Affymetrix GeneChip Scanner 3000 TG.
|
Data processing |
Mouse Gene 1.0 ST arrays were normalized using RMA16 implemented in the GeneSpring 12.6.
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|
|
Submission date |
Apr 09, 2019 |
Last update date |
Apr 11, 2022 |
Contact name |
Satoshi Watanabe |
E-mail(s) |
watanabe.satoshi.7r@kyoto-u.ac.jp
|
Phone |
81757514160
|
Organization name |
Kyoto University
|
Department |
Graduate School of Medicine
|
Lab |
Department of Molecular Genetics
|
Street address |
53, Kawahara-cho, Shogoin, Sakyo
|
City |
Kyoto |
State/province |
Kyoto |
ZIP/Postal code |
606-8507 |
Country |
Japan |
|
|
Platform ID |
GPL6246 |
Series (2) |
GSE129546 |
Induced expression of PD-L1 in spermatogonial stem cells by self-renewal division allows allogeneic offspring production [microarray] |
GSE129659 |
Induced expression of PD-L1 in spermatogonial stem cells by self-renewal division allows allogeneic offspring production |
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