|
| Status |
Public on Apr 11, 2022 |
| Title |
3week-rep1 |
| Sample type |
RNA |
| |
|
| Source name |
SSCs cultured for 3 weeks
|
| Organism |
Mus musculus |
| Characteristics |
cell type: spermatogonial stem cells (SSCs)
|
| Treatment protocol |
adult DBA/2 testicular cells purified by magnetically sorted using anti-CD9 antibody
|
| Growth protocol |
DBA/2 GS cells and adult DBA/2 testicular cells purified by magnetically sorted using anti-CD9 antibody were cultured in culture medium based on Iscove’s modified MEM (Invitrogen, Carlsbad, CA), which was supplemented with 10 ng/ml human FGF2, 15 ng/ml recombinant rat GDNF (both from Peprotech, London, UK) and 1% fetal bovine serum (FBS) (Kanatsu-Shinohara et al, 2014).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted by TRIzol (Qiagen) and purified by RNeasy mini kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
GeneChip WT Terminal Labeling Kit (Affymetrix) were used according to Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Fragmented and labelled cDNA was hybridized to GeneChip Mouse Gene 1.0 ST (Affymetrix).
|
| Scan protocol |
Arrays were scanned using the Affymetrix GeneChip Scanner 3000 TG.
|
| Data processing |
Mouse Gene 1.0 ST arrays were normalized using RMA16 implemented in the GeneSpring 12.6.
|
| |
|
| Submission date |
Apr 09, 2019 |
| Last update date |
Apr 11, 2022 |
| Contact name |
Satoshi Watanabe |
| E-mail(s) |
watanabe.satoshi.7r@kyoto-u.ac.jp
|
| Phone |
81757514160
|
| Organization name |
Kyoto University
|
| Department |
Graduate School of Medicine
|
| Lab |
Department of Molecular Genetics
|
| Street address |
53, Kawahara-cho, Shogoin, Sakyo
|
| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE129546 |
Induced expression of PD-L1 in spermatogonial stem cells by self-renewal division allows allogeneic offspring production [microarray] |
| GSE129659 |
Induced expression of PD-L1 in spermatogonial stem cells by self-renewal division allows allogeneic offspring production |
|
