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Series GSE120701 Query DataSets for GSE120701
Status Public on Dec 31, 2019
Title Developmentally controlled expression of Cxcr4 by hematopoietic progenitors allows tracing of monocyte function in stroke
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Monocyte-derived and tissue-resident macrophages, such as microglia, belong to independent lineages with distinct functions in steady state. During inflammation, however, changes in phenotypes and transcriptional profiles make it difficult to assess their respective roles in pathology. Here, we report that developmentally controlled expression of Cxcr4 by hematopoietic progenitors allows highly specific inducible lineage tracing of hematopoietic stem cell-derived monocytes during inflammation. In two stroke models, we found that monocytes recruited to the brain were confined to the lesion and its surrounding during the acute phase. However, monocytes did not engraft in peri-lesional tissue, but were enclosed in scar tissue by microglia and astrocytes after completed repair. Transcriptional profiles of brain monocytes and microglia reflect major differences in signaling pathways, paracrine mediators, and proliferative behavior. Initial recruitment and subsequent enclosure of monocytes required Cxcr4. Our data show that CXCR4 signaling regulates a specific monocyte-mediated contribution to injury and repair in stroke.
Overall design Cxcr4-GFP mice 2 populations (monocytes and microglia) 3d post permanent photothrombosis (n=4) & Cxcr4 conditional KO (Cxcr4CreER KO) and wt mice (Cxcr4CreER contol) 2 populations each (monocytes and microglia) 3d post permanent photothrombosis (n=3)
Contributor(s) Werner Y, Mass E
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Submission date Oct 01, 2018
Last update date Dec 31, 2019
Contact name Joachim Schultze
Organization name LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
Department Genomics and Immunoregulation
Street address Carl-Troll-Strasse 31
City Bonn
State/province NRW
ZIP/Postal code 53115
Country Germany
Platforms (2)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (20)
GSM3408715 18774+18772 BrKo_ GFP-negativ
GSM3408716 18774+18772 BrKo_ GFP-positiv
GSM3408717 5xpool BrKo_ GFP-negativ
BioProject PRJNA494171
SRA SRP163038

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Supplementary file Size Download File type/resource
GSE120701_norm_gene_counts_Cxcr4CreKO.txt.gz 789.8 Kb (ftp)(http) TXT
GSE120701_norm_gene_counts_GFP.txt.gz 412.7 Kb (ftp)(http) TXT
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Processed data are available on Series record

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