Expression profiling by high throughput sequencing Non-coding RNA profiling by high throughput sequencing
Summary
Systematic evaluation of the cell populations of the intestinal crypt confirms the plasticity of the EEC progenitors, and uncovering of a functional role for the evolutionarily consvered miRNA-7 in this context.
Overall design
Separation of mouse intestinal crypts into subpopulations based on expression of Sox9-EGFP, LgR5, or Prox1. Fruit fly midgut was dissected prior to and at various time point following infection by Ecc15. Single cell RNA-seq of enteroids treated by LNAs.
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