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Series GSE117341 Query DataSets for GSE117341
Status Public on Sep 27, 2018
Title Repressive mark H3K27me3 in PRMT6 KO NT2/D1 cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Protein arginine methyltransferase 6 (PRMT6) is an epigenetic regulator of fundamental cellular processes, such as gene expression and DNA repair. Asymmetric dimethylation of histone H3 at arginine 2 (H3R2me2a) is the major histone modification catalyzed by PRMT6. To identify the genome-wide deposition and transcriptional impact of H3R2me2a, we established PRMT6 deletion in a human cell model of neural differentiation. These knockout cells show severe neural differentiation defects. ChIP-seq profiling reveals that H3R2me2a is present at promoter as well as non-promoter sites in a PRMT6-dependent manner. Loss of H3R2me2a causes enhanced H3K4me3 deposition and target gene transcription supporting a genome-wide repressive nature of H3R2me2a. Intriguingly, the non-promoter H3R2me2a peaks co-localize with active enhancer marks, such as H3K4me1 and H3K27ac.
Overall design Examining of PRMT6 influence on H3K27me3 deposition in CRISPR control and knockout PRMT6 with neighboring histone modification.
Contributor(s) Sahu P, Bouchard C, Bauer U
Citation(s) 30232013
Submission date Jul 18, 2018
Last update date Dec 30, 2018
Contact name Uta Maria Bauer
Organization name Philipps University Marburg
Department Institute of Molecular Biology and Tumor Research
Lab AG Bauer
Street address Hans-Meerwein-Str. 2
City Marburg
State/province Hessen
ZIP/Postal code 35043
Country Germany
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (4)
GSM3291465 NT2D1_CT_H3K27me3_-RA
GSM3291466 NT2D1_KO_H3K27me3_-RA
GSM3291467 NT2D1_CT_H3K27me3_+RA
This SubSeries is part of SuperSeries:
GSE107612 Genomic location of PRMT6-dependent H3R2 methylation is decisive for the transcriptional outcome of associated genes
BioProject PRJNA481784
SRA SRP154387

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Supplementary file Size Download File type/resource
GSE117341_RAW.tar 639.1 Mb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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