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| Status |
Public on Sep 27, 2018 |
| Title |
Repressive mark H3K27me3 in PRMT6 KO NT2/D1 cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Protein arginine methyltransferase 6 (PRMT6) is an epigenetic regulator of fundamental cellular processes, such as gene expression and DNA repair. Asymmetric dimethylation of histone H3 at arginine 2 (H3R2me2a) is the major histone modification catalyzed by PRMT6. To identify the genome-wide deposition and transcriptional impact of H3R2me2a, we established PRMT6 deletion in a human cell model of neural differentiation. These knockout cells show severe neural differentiation defects. ChIP-seq profiling reveals that H3R2me2a is present at promoter as well as non-promoter sites in a PRMT6-dependent manner. Loss of H3R2me2a causes enhanced H3K4me3 deposition and target gene transcription supporting a genome-wide repressive nature of H3R2me2a. Intriguingly, the non-promoter H3R2me2a peaks co-localize with active enhancer marks, such as H3K4me1 and H3K27ac.
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| Overall design |
Examining of PRMT6 influence on H3K27me3 deposition in CRISPR control and knockout PRMT6 with neighboring histone modification.
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| Contributor(s) |
Sahu P, Bouchard C, Bauer U |
| Citation(s) |
30232013 |
| |
| Submission date |
Jul 18, 2018 |
| Last update date |
Dec 30, 2018 |
| Contact name |
Uta Maria Bauer |
| E-mail(s) |
bauer@imt.uni-marburg.de
|
| Organization name |
Philipps University Marburg
|
| Department |
Institute of Molecular Biology and Tumor Research
|
| Lab |
AG Bauer
|
| Street address |
Hans-Meerwein-Str. 2
|
| City |
Marburg |
| State/province |
Hessen |
| ZIP/Postal code |
35043 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE107612 |
Genomic location of PRMT6-dependent H3R2 methylation is decisive for the transcriptional outcome of associated genes |
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| Relations |
| BioProject |
PRJNA481784 |
| SRA |
SRP154387 |
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