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Platform GPL3050 Query DataSets for GPL3050
Status Public on Dec 09, 2005
Title Human Unigene3.1 cDNA Array 37.5K v1.0
Technology type spotted DNA/cDNA
Distribution non-commercial
Organism Homo sapiens
Manufacturer Gene expression profiling group, Division of Molecular Genome Analysis, DKFZ Heidelberg
Manufacture protocol The microarrays carried 37,530 human cDNA clones (human UniGene Set RZPD3.1, German Resource Center for Genome Research, Berlin, Germany). DNA fragments were amplified by PCR and spotted on epoxysilane glass slides (Quantifoil, Jena, Germany) using the VersArray ChipWriter Pro System (Biorad, Munich, Germany) and SMP3 pins (Telechem, Sunnyvale, CA, USA). Spotting was performed in the division of Molecular Genome Analysis with a
spacing of 28x29 Spots in 4x12 Subarrays (top-down to left-right). After spotting, microarrays were rehydrated, and the DNA was denatured with boiling water prior to washing with 0.2% SDS, water, ethanol, and isopropanol. The arrays were dried with air pressure.
Support glass
Coating epoxysilane
 
Description The human Unigene3.1 cDNA Array represents 37530 cDNA clones provided by "Deutsches Ressourcenzentrum fuer Genomforschung (RZPD)"
 
Web link http://www.dkfz.de/mga/groups.asp?siteID=93
Contributor(s) Sultmann H, Kuner R, Buness A, Ruschhaupt M, Finis K, Stojanov M, Poustka A
Submission date Nov 07, 2005
Last update date Jan 17, 2013
Contact name Ruprecht Kuner
Organization name German Cancer Research Center and National Center of Tumor Diseases
Department Molecular Genetics
Lab Unit Cancer Genome Research
Street address Im Neuenheimer Feld 460
City Heidelberg
ZIP/Postal code 69120
Country Germany
 
Samples (303) GSM82393, GSM82394, GSM82395, GSM82396, GSM82397, GSM82398 
Series (8)
GSE3586 Dilated Cardiomyopathy and Non Failing Septal Biopsies
GSE3698 Arthritis_RA_OA_VNS
GSE3699 DNA-Methyltransferases (DNMT) knockouts

Data table header descriptions
ID RZPD ID
PLATE_ID Plate identifier
ROW_ID Row within plate
COLUMN_ID Column within plate
GB_LIST GenBank or RefSeq accession
CLONE_ID IMAGE ID
SPOT_ID
RZPD_LIB_983_ID alternative RZPD clone ID
VECTOR cloning vector
ANTIBIOTIC_RESISTENCE E.coli growth medium restistence
PCR_PRIMER cDNA insert amplification primer
RESTRICTION_ENZYMES enzymes for cDNA insert restriction sites
GENEBANK_ACC Genebank Ids annotated by the clone provider RZPD Berlin May 2005
GENE_SYMBOL Gene symbol from Unigene Cluster annotated by the clone provider RZPD Berlin May 2005
GENE_DESCRIPTION Gene description annotated by the clone provider RZPD Berlin May 2005
CHROMOSOME cytogenetic information annotated by the clone provider RZPD Berlin May 2005
UNIGENE_ID Unigene ID annotated by the clone provider RZPD Berlin May 2005
REFSEQ_ID RefSeq_ID annotated by the clone provider RZPD Berlin May 2005
ENSEMBL_ID Ensembl ID annotated by the clone provider RZPD Berlin May 2005
CLONE_QUALITY Clone quality annotated by the clone provider RZPD Berlin May 2005
order

Data table
ID PLATE_ID ROW_ID COLUMN_ID GB_LIST CLONE_ID SPOT_ID RZPD_LIB_983_ID VECTOR ANTIBIOTIC_RESISTENCE PCR_PRIMER RESTRICTION_ENZYMES GENEBANK_ACC GENE_SYMBOL GENE_DESCRIPTION CHROMOSOME UNIGENE_ID REFSEQ_ID ENSEMBL_ID CLONE_QUALITY order
IMAGp998A1188 1 A 1 NM_182639 NM_182638 NM_182637 NM_000195 IMAGE:111634 HU3_p983A011D pT7T3D-PacI ampicillin RZPD M13 forward/reverse EcoRI,NotI T90991,T84567,BX096968 HPS1 Hermansky-Pudlak syndrome 1 10:q23.1-q23.3 Hs.404568 NM_182639,NM_182638,NM_182637,NM_000195 ENSG00000107521 + 1
IMAGp998H1180 1 A 2 T72745 T72671 BX092862 IMAGE:108730 HU3_p983A012D pT7T3D-PacI ampicillin RZPD M13 forward/reverse EcoRI,NotI T72745,T72671,BX092862 - 2
IMAGp998A0786 1 A 3 T90271 T82849 BX097001 AF339765 IMAGE:110862 HU3_p983A021D pT7T3D-PacI ampicillin RZPD M13 forward/reverse EcoRI,NotI T90271,T82849,BX097001,AF339765 Clone IMAGE:110862, mRNA sequence 13 Hs.225914 o 3
IMAGp998C0318 1 A 4 T66870 T66869 BX092893 IMAGE:66362 HU3_p983A022D pT7T3D-PacI ampicillin RZPD M13 forward/reverse EcoRI,NotI T66870,T66869,BX092893 Transcribed locus, moderately similar to XP_509882.1 LOC452831 [Pan troglodytes] 18 Hs.263209 o 4
IMAGp998A0785 1 A 5 T82802 T89979 BX097039 IMAGE:110478 HU3_p983A031D pT7T3D-PacI ampicillin RZPD M13 forward/reverse EcoRI,NotI T82802,T89979,BX097039 Transcribed locus 1 Hs.187420 o 5
IMAGp998A0618 1 A 6 NM_005319 IMAGE:66317 HU3_p983A032D pT7T3D-PacI ampicillin RZPD M13 forward/reverse EcoRI,NotI T66816,T66815,BX092939 HIST1H1C Histone 1, H1c 6:p21.3 Hs.7644 NM_005319 ENSG00000187837 ++ 6
IMAGp998F1684 1 A 7 NM_000108 IMAGE:110223 HU3_p983A041D pT7T3D-PacI ampicillin RZPD M13 forward/reverse EcoRI,NotI T71416,T71280,BX097058 DLD Dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex) 7:q31-q32 Hs.131711 NM_000108 ENSG00000091140 ++ 7
IMAGp998A0518 1 A 8 T66795 T66794 BX092976 IMAGE:66316 HU3_p983A042D pT7T3D-PacI ampicillin RZPD M13 forward/reverse EcoRI,NotI T66795,T66794,BX092976 - 8
IMAGp998A0784 1 A 9 XM_496780 IMAGE:110094 HU3_p983A051D pT7T3D-PacI ampicillin RZPD M13 forward/reverse EcoRI,NotI T81624,T71133,BX097092 FLJ38109 Hypothetical protein FLJ38109 5:q33.2 Hs.551597 XM_496780 + 9
IMAGp998A1018 1 A 10 NM_005643 IMAGE:66321 HU3_p983A052D pT7T3D-PacI ampicillin RZPD M13 forward/reverse EcoRI,NotI T66818,T66817,BX093017 TAF11 TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28kDa 6:p21.31 Hs.112444 NM_005643 ENSG00000064995 + 10
IMAGp998B1885 1 A 11 NM_018559 IMAGE:110513 HU3_p983A061D pT7T3D-PacI ampicillin RZPD M13 forward/reverse EcoRI,NotI T90181,T82990,BX097960 KIAA1704 KIAA1704 13:q13-q14 Hs.507922 NM_018559 ENSG00000133114 o 11
IMAGp998G1681 1 A 12 T80716 BX093941 IMAGE:109095 HU3_p983A062D pT7T3D-PacI ampicillin RZPD M13 forward/reverse EcoRI,NotI T80716,BX093941 Transcribed locus 14 Hs.161790 ++ 12
IMAGp998D1383 1 A 13 NM_006598 IMAGE:109788 HU3_p983A071D pT7T3D-PacI ampicillin RZPD M13 forward/reverse EcoRI,NotI T82115,T81795,BX097989 SLC12A7 Solute carrier family 12 (potassium/chloride transporters), member 7 5:p15 Hs.172613 NM_006598 ENSG00000113504 ++ 13
IMAGp998B1418 1 A 14 NM_018490 IMAGE:66349 HU3_p983A072D pT7T3D-PacI ampicillin RZPD M13 forward/reverse EcoRI,NotI T66858,T66857,BX093974 LGR4 Leucine-rich repeat-containing G protein-coupled receptor 4 11:p14-p13 Hs.502176 NM_018490 + 14
IMAGp998H2081 1 A 15 NM_207400 IMAGE:109123 HU3_p983A081D pT7T3D-PacI ampicillin RZPD M13 forward/reverse EcoRI,NotI T80979,T80978,BX098029 FLJ39739 FLJ39739 protein,Transcribed locus 1:q21.1,1 Hs.406990, Hs.487721 NM_207400 ENSG00000178669 + 15
IMAGp998C1818 1 A 16 NM_002809 IMAGE:66377 HU3_p983A082D pT7T3D-PacI ampicillin RZPD M13 forward/reverse EcoRI,NotI T66907,T66906,BX093997 PSMD3 Proteasome (prosome, macropain) 26S subunit, non-ATPase, 3 17:q21.1 Hs.12970 NM_002809 ENSG00000108344 o 16
IMAGp998J2084 1 A 17 NM_006654 IMAGE:110323 HU3_p983A091D pT7T3D-PacI ampicillin RZPD M13 forward/reverse EcoRI,NotI T82029,T71575,BX098070 FRS2 Fibroblast growth factor receptor substrate 2 12:q15 Hs.334831 NM_006654 ENSG00000166225 + 17
IMAGp998A1918 1 A 18 T66806 T66805 BX094022 IMAGE:66330 HU3_p983A092D pT7T3D-PacI ampicillin RZPD M13 forward/reverse EcoRI,NotI T66806,T66805,BX094022 - 18
IMAGp998A2081 1 A 19 NM_014270 IMAGE:108955 HU3_p983A101D pT7T3D-PacI ampicillin RZPD M13 forward/reverse EcoRI,NotI T80665,BX098139 SLC7A9 Solute carrier family 7 (cationic amino acid transporter, y+ system), member 9 19:q13.1 Hs.408567 NM_014270 ENSG00000021488 ++ 19
IMAGp998A1618 1 A 20 XM_114735 IMAGE:66327 HU3_p983A102D pT7T3D-PacI ampicillin RZPD M13 forward/reverse EcoRI,NotI T66824,T66823,BX094042 CR1L Complement component (3b/4b) receptor 1-like 1:q32.1 Hs.149414 XM_114735 ENSG00000197721 ++ 20

Total number of rows: 37530

Table truncated, full table size 8773 Kbytes.






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