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Series GSE12291 Query DataSets for GSE12291
Status Public on Jul 29, 2009
Title Reconstruction of gene interaction networks by in vitro RNA interference and global expression analysis
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We here used the technology of RNA interference or selective treatment of the ESR1 and cDNA microarrays to reconstruct a network between genes known to be disregulated in ER-positive breast cancer diseases. Therefor single genes of high importance were silenced and the effect of this pertubation was measured with cDNA microarrays.

Keywords: Network Reconstruction, RNAi, siRNA, Interaction Network
 
Overall design human ER-positive breast cancer cell line was cultivated as described and transfection with chemically synthesized siRNAs was performed according to standard HiPerFect transfection protocol by Qiagen (Hilden, Germany) At least 4 cDNA-microarrays were hybridized in dye-swap method for the silencing of every gene of interest. To minimize off-target effects we used at least two different siRNAs per gene.
 
Contributor(s) Fröhlich H, Ruschhaupt M, Kuner R, Sültmann H
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Submission date Jul 30, 2008
Last update date Jan 17, 2013
Contact name Mark Fellmann
E-mail(s) m.fellmann@dkfz.de
Organization name DKFZ
Department Molecular Genome Analysis
Street address INF 580
City Heidelberg
ZIP/Postal code 69120
Country Germany
 
Platforms (1)
GPL3050 Human Unigene3.1 cDNA Array 37.5K v1.0
Samples (78)
GSM309238 MCF7 ESRalpha positive breast cancer cell line, siRNA mediated knock-down AKT1 Replicate 1
GSM309239 MCF7 ESRalpha positive breast cancer cell line, siRNA mediated knock-down AKT1 Replicate 1 dyeswap
GSM309240 MCF7 ESRalpha positive breast cancer cell line, siRNA mediated knock-down AKT1 Replicate 2
Relations
BioProject PRJNA113581

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE12291_RAW.tar 237.1 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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