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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 01, 2006 |
| Title |
DNA-Methyltransferases (DNMT) knockouts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Alterations in DNA-methylation are a hallmark of human tumor cells. DNMT1 is expressed in three different splice variants and exhibits considerable preference for hemimethylated DNA and is therefore referred to as the maintenance methylase. DNMT3b is generally considered to be responsible for de novo methylation during embryonic development and probably also during tumor development. Recent studies using somatic knock-out of DNA-methyltransferases have shown a reduction in overall methylation and a re-expression of tumor suppressor genes. Cells deficient of both, DNMT1 and DNMT3b may exhibit almost complete demethylation and growth suppression. In the present study, we performed MEK-inhibition via U0126 treatment for the wildtype and every DNMT-knockout to focus on MEK/RAS-target genes regulated by methylation.
Keywords: genetic modification
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| Overall design |
This series includes dual channel hybridizations of 14 independent HCT-116
cell line lysates on Unigene3.1 cDNA Arrays. In this series, five DNMT-wildtype, three DNMT1-KO, three DNMT3-KO and three DNMT1 and 3B double KO were included with different treatment status (none, DMSO, U0126, Aza-CdR). For every independent sample, two technical replicates were performed and further processed as mean values.
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| Contributor(s) |
Kuner R, Sultmann H, Buness A, Ruschhaupt M, Poustka A, Sers C, Lund P, Gyorffy B, Schafer R |
| Citation(s) |
19569244 |
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| Submission date |
Nov 30, 2005 |
| Last update date |
Jan 17, 2013 |
| Contact name |
Ruprecht Kuner |
| Organization name |
German Cancer Research Center and National Center of Tumor Diseases
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| Department |
Molecular Genetics
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| Lab |
Unit Cancer Genome Research
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| Street address |
Im Neuenheimer Feld 460
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| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL3050 |
Human Unigene3.1 cDNA Array 37.5K v1.0 |
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| Samples (14)
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| GSM85452 |
P200 HCT-116, DNMT wildtype, non-treated |
| GSM85453 |
P201 HCT-116, DNMT wildtype, DMSO-treated |
| GSM85454 |
P202 HCT-116, DNMT wildtype, U0126-treated |
| GSM85455 |
P203 HCT-116, DNMT wildtype, Aza-treated |
| GSM85456 |
P204 HCT-116, DNMT wildtype, Aza-treated |
| GSM85457 |
P205 HCT-116, DNMT1 knockout, non-treated |
| GSM85458 |
P206 HCT-116, DNMT1 knockout, DMSO-treated |
| GSM85459 |
P207 HCT-116, DNMT1 knockout, U0126-treated |
| GSM85460 |
P208 HCT-116, DNMT3B knockout, non-treated |
| GSM85461 |
P209 HCT-116, DNMT3B knockout, DMSO-treated |
| GSM85462 |
P210 HCT-116, DNMT3B knockout, U0126-treated |
| GSM85463 |
P211 HCT-116, DNMT1 and 3B double knockout, non-treated |
| GSM85464 |
P212 HCT-116, DNMT1 and 3B double knockout, DMSO-treated |
| GSM85465 |
P213 HCT-116, DNMT1 and 3B double knockout, U0126-treated |
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| Relations |
| BioProject |
PRJNA93849 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE3699_RAW.tar |
89.5 Mb |
(http)(custom) |
TAR (of GPR) |
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