Akap9 A kinase anchor protein 9 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Akap9provided by MGI
Official Full Name: A kinase anchor protein 9provided by MGI
Primary source: MGI:MGI:2178217
See related: Ensembl:ENSMUSG00000040407 AllianceGenome:MGI:2178217
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: PRKA9; AKAP-9; mei2-5; AKAP450; repro12; G1-448-15; mKIAA0803; 5730481H23Rik
Summary: Predicted to enable several functions, including potassium channel regulator activity; protein kinase A regulatory subunit binding activity; and transmembrane transporter binding activity. Acts upstream of or within Sertoli cell development and spermatogenesis. Located in several cellular components, including 9+2 motile cilium; microtubule organizing center; and subapical part of cell. Is expressed in several structures, including brain; gut; heart; skeletal muscle; and urinary system. Human ortholog(s) of this gene implicated in long QT syndrome 11. Orthologous to human AKAP9 (A-kinase anchoring protein 9). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in CNS E14 (RPKM 12.4), CNS E18 (RPKM 10.9) and 23 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 A1; 5 2.26 cM

Exon count:: 52

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (3977410..4130204)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (3927736..4080204)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Nesprin-1alpha recruits Akap450 to the nuclear envelope (NE)independently of kinesin and that Akap450, but not other centrosomal proteins, is required for microtubule nucleation from the NE.
Title: Nesprin-1α-Dependent Microtubule Nucleation from the Nuclear Envelope via Akap450 Is Necessary for Nuclear Positioning in Muscle Cells.
AKAP9 and, by inference, the regulation of the microtubule network are critical for blood-testis barrier function and subsequent germ cell development during spermatogenesis
Title: AKAP9, a Regulator of Microtubule Dynamics, Contributes to Blood-Testis Barrier Function.
AKAP9 is dispensable for T cell priming and migration into tissues and lymph nodes, but is required for T cell retention in tissues.
Title: AKAP9 regulates activation-induced retention of T lymphocytes at sites of inflammation.
We postulate that this novel interaction linking MVI with the PKA pathway could be important for targeting AKAP9-PKA complex within cells and/or providing PKA to phosphorylate MVI tail domain.
Title: A Kinase Anchoring Protein 9 Is a Novel Myosin VI Binding Partner That Links Myosin VI with the PKA Pathway in Myogenic Cells.
Overexpression of an AKAP450 fragment containing the CK1delta-binding site inhibits Golgi-derived microtubule nucleation, Golgi distribution of intraflagellar transport protein 20 homologue, and ciliogenesis.
Title: Casein kinase 1δ functions at the centrosome and Golgi to promote ciliogenesis.
Mutations in Akap9 disrupt Sertoli cell maturation, gap and tight junctions and localization of zona occluden-1 and connexin43.
Title: AKAP9 is essential for spermatogenesis and sertoli cell maturation in mice.
in heart, Yotiao brings together PKA, PP1, PDE4D3, AC9, and the I(Ks) channel to achieve localized temporal regulation of beta-adrenergic stimulation.
Title: The A-kinase anchoring protein Yotiao facilitates complex formation between adenylyl cyclase type 9 and the IKs potassium channel in heart.
Expression of Akap9 in the mouse striatum is modulated by a sequence variant (B2 SINE indel) in the 3' UTR of Comt (catechol-O-methyltransferase).
Title: A transposon in Comt generates mRNA variants and causes widespread expression and behavioral differences among mice.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (1)
Chemically induced (other) (1) 1 citation
Endonuclease-mediated (2)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH137133 (e-PCR)
- UniSTS:219221

RH126484 (e-PCR)
- UniSTS:163192

AW545847 (e-PCR)
- UniSTS:179132

AKAP9 (e-PCR)
- UniSTS:277956

RH140917 (e-PCR)
- UniSTS:222931

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables molecular adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables molecular adaptor activity	ISO Inferred from Sequence Orthology more info
enables molecular adaptor activity	ISS Inferred from Sequence or Structural Similarity more info
enables potassium channel regulator activity	IBA Inferred from Biological aspect of Ancestor more info
enables potassium channel regulator activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase A regulatory subunit binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase A regulatory subunit binding	ISO Inferred from Sequence Orthology more info	PubMed
enables transmembrane transporter binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within Sertoli cell development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to cAMP	ISO Inferred from Sequence Orthology more info
involved_in maintenance of centrosome location	IBA Inferred from Biological aspect of Ancestor more info
involved_in maintenance of centrosome location	ISO Inferred from Sequence Orthology more info
involved_in maintenance of centrosome location	ISS Inferred from Sequence or Structural Similarity more info
involved_in microtubule nucleation	IBA Inferred from Biological aspect of Ancestor more info
involved_in microtubule nucleation	ISO Inferred from Sequence Orthology more info
involved_in microtubule nucleation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of microtubule polymerization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of peptidyl-serine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of potassium ion transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
involved_in protein-containing complex localization	ISO Inferred from Sequence Orthology more info	PubMed
involved_in regulation of Golgi organization	ISO Inferred from Sequence Orthology more info
involved_in regulation of Golgi organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of cardiac muscle cell action potential involved in regulation of contraction	ISO Inferred from Sequence Orthology more info
involved_in regulation of heart rate by cardiac conduction	ISO Inferred from Sequence Orthology more info
involved_in regulation of membrane repolarization	ISO Inferred from Sequence Orthology more info
involved_in regulation of ventricular cardiac muscle cell membrane repolarization	ISO Inferred from Sequence Orthology more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in 9+2 motile cilium	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in Golgi apparatus	ISS Inferred from Sequence or Structural Similarity more info
is_active_in Golgi stack	IBA Inferred from Biological aspect of Ancestor more info
located_in Golgi stack	ISO Inferred from Sequence Orthology more info
located_in Golgi stack	ISS Inferred from Sequence or Structural Similarity more info
is_active_in centrosome	IBA Inferred from Biological aspect of Ancestor more info
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	ISO Inferred from Sequence Orthology more info	PubMed
located_in ciliary basal body	IDA Inferred from Direct Assay more info	PubMed
is_active_in cis-Golgi network	IBA Inferred from Biological aspect of Ancestor more info
located_in cis-Golgi network	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic branch	ISO Inferred from Sequence Orthology more info
is_active_in extrinsic component of postsynaptic density membrane	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in pericentriolar material	IDA Inferred from Direct Assay more info	PubMed
part_of potassium channel complex	ISO Inferred from Sequence Orthology more info	PubMed
located_in subapical part of cell	IDA Inferred from Direct Assay more info	PubMed
is_active_in synaptic membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in synaptic membrane	ISO Inferred from Sequence Orthology more info
part_of voltage-gated potassium channel complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: A-kinase anchor protein 9

Names: A kinase (PRKA) anchor protein (yotiao) 9; protein kinase A-anchoring protein 9

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_194462.2 → NP_919444.2 A-kinase anchor protein 9

Status: VALIDATED

Source sequence(s)

AC027653, AC064793, AJ582913, CJ174318

Consensus CDS

CCDS19070.1

UniProtKB/Swiss-Prot

Q3TH74, Q70FJ1, Q80TR6

UniProtKB/TrEMBL

E9QQ10

Related

ENSMUSP00000046129.9, ENSMUST00000044492.10

Conserved Domains (4) summary

COG4942
Location:114 → 374

EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]

pfam10495
Location:3576 → 3657

PACT_coil_coil; Pericentrin-AKAP-450 domain of centrosomal targeting protein

pfam15905
Location:503 → 796

HMMR_N; Hyaluronan mediated motility receptor N-terminal

cl12057
Location:673 → 734

Seryl_tRNA_N; Seryl-tRNA synthetase N-terminal domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

3977410..4130204

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006503518.5 → XP_006503581.1 A-kinase anchor protein 9 isoform X1

UniProtKB/Swiss-Prot

Q3TH74, Q70FJ1, Q80TR6

Conserved Domains (5) summary

COG0419
Location:3 → 418

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

COG1196
Location:268 → 972

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:1871 → 2533

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam10495
Location:3607 → 3688

PACT_coil_coil; Pericentrin-AKAP-450 domain of centrosomal targeting protein

pfam17380
Location:1523 → 1708

DUF5401; Family of unknown function (DUF5401)
XM_006503520.5 → XP_006503583.1 A-kinase anchor protein 9 isoform X3

UniProtKB/Swiss-Prot

Q3TH74, Q70FJ1, Q80TR6

Conserved Domains (5) summary

COG0419
Location:3 → 418

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

COG1196
Location:268 → 972

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:1871 → 2533

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam10495
Location:3607 → 3688

PACT_coil_coil; Pericentrin-AKAP-450 domain of centrosomal targeting protein

pfam17380
Location:1523 → 1708

DUF5401; Family of unknown function (DUF5401)
XM_006503523.5 → XP_006503586.1 A-kinase anchor protein 9 isoform X8

UniProtKB/Swiss-Prot

Q3TH74, Q70FJ1, Q80TR6

Conserved Domains (6) summary

COG0419
Location:3 → 418

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

COG1196
Location:268 → 972

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:1808 → 2122

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

TIGR02169
Location:2161 → 2477

SMC_prok_A; chromosome segregation protein SMC, primarily archaeal type

pfam10495
Location:3547 → 3628

PACT_coil_coil; Pericentrin-AKAP-450 domain of centrosomal targeting protein

pfam17380
Location:1523 → 1708

DUF5401; Family of unknown function (DUF5401)
XM_006503519.5 → XP_006503582.1 A-kinase anchor protein 9 isoform X2

UniProtKB/Swiss-Prot

Q3TH74, Q70FJ1, Q80TR6

Conserved Domains (6) summary

COG0419
Location:3 → 418

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

COG1196
Location:268 → 972

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:1809 → 2520

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

TIGR02169
Location:1862 → 2212

SMC_prok_A; chromosome segregation protein SMC, primarily archaeal type

pfam10495
Location:3594 → 3675

PACT_coil_coil; Pericentrin-AKAP-450 domain of centrosomal targeting protein

pfam17380
Location:1523 → 1708

DUF5401; Family of unknown function (DUF5401)
XM_017320586.3 → XP_017176075.1 A-kinase anchor protein 9 isoform X5

UniProtKB/Swiss-Prot

Q3TH74, Q70FJ1, Q80TR6

Conserved Domains (6) summary

COG0419
Location:3 → 418

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

COG1196
Location:268 → 972

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:1809 → 2520

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

TIGR02169
Location:1862 → 2212

SMC_prok_A; chromosome segregation protein SMC, primarily archaeal type

pfam10495
Location:3594 → 3675

PACT_coil_coil; Pericentrin-AKAP-450 domain of centrosomal targeting protein

pfam17380
Location:1523 → 1708

DUF5401; Family of unknown function (DUF5401)
XM_017320587.3 → XP_017176076.1 A-kinase anchor protein 9 isoform X9

UniProtKB/Swiss-Prot

Q3TH74, Q70FJ1, Q80TR6

Conserved Domains (6) summary

COG0419
Location:3 → 418

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

COG1196
Location:268 → 972

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:2092 → 2446

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

TIGR02169
Location:1862 → 2212

SMC_prok_A; chromosome segregation protein SMC, primarily archaeal type

pfam10495
Location:3534 → 3615

PACT_coil_coil; Pericentrin-AKAP-450 domain of centrosomal targeting protein

pfam17380
Location:1523 → 1708

DUF5401; Family of unknown function (DUF5401)
XM_017320588.3 → XP_017176077.1 A-kinase anchor protein 9 isoform X10

UniProtKB/Swiss-Prot

Q3TH74, Q70FJ1, Q80TR6

Conserved Domains (6) summary

COG0419
Location:3 → 418

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

COG1196
Location:268 → 972

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:2092 → 2446

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

TIGR02169
Location:1862 → 2212

SMC_prok_A; chromosome segregation protein SMC, primarily archaeal type

pfam10495
Location:3534 → 3615

PACT_coil_coil; Pericentrin-AKAP-450 domain of centrosomal targeting protein

pfam17380
Location:1523 → 1708

DUF5401; Family of unknown function (DUF5401)
XM_006503522.5 → XP_006503585.1 A-kinase anchor protein 9 isoform X7

UniProtKB/Swiss-Prot

Q3TH74, Q70FJ1, Q80TR6

Conserved Domains (5) summary

COG0419
Location:3 → 418

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

COG1196
Location:268 → 972

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:1824 → 2486

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam10495
Location:3560 → 3641

PACT_coil_coil; Pericentrin-AKAP-450 domain of centrosomal targeting protein

pfam12128
Location:1544 → 2073

DUF3584; Protein of unknown function (DUF3584)
XM_006503524.5 → XP_006503587.1 A-kinase anchor protein 9 isoform X11

UniProtKB/Swiss-Prot

Q3TH74, Q70FJ1, Q80TR6

Conserved Domains (4) summary

COG0419
Location:3 → 418

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

COG1196
Location:268 → 972

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:1753 → 2415

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam10495
Location:3489 → 3570

PACT_coil_coil; Pericentrin-AKAP-450 domain of centrosomal targeting protein
XM_036164709.1 → XP_036020602.1 A-kinase anchor protein 9 isoform X13

UniProtKB/Swiss-Prot

Q3TH74, Q70FJ1, Q80TR6

Conserved Domains (4) summary

COG0419
Location:3 → 418

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

COG1196
Location:268 → 972

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:1753 → 2415

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam10495
Location:3489 → 3570

PACT_coil_coil; Pericentrin-AKAP-450 domain of centrosomal targeting protein
XM_036164708.1 → XP_036020601.1 A-kinase anchor protein 9 isoform X12

UniProtKB/Swiss-Prot

Q3TH74, Q70FJ1, Q80TR6

Conserved Domains (5) summary

COG0419
Location:3 → 418

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

COG1196
Location:268 → 972

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:1691 → 2402

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam17380
Location:1383 → 1590

DUF5401; Family of unknown function (DUF5401)

pfam10495
Location:3476 → 3557

PACT_coil_coil; Pericentrin-AKAP-450 domain of centrosomal targeting protein
XM_006503521.5 → XP_006503584.1 A-kinase anchor protein 9 isoform X4

UniProtKB/Swiss-Prot

Q3TH74, Q70FJ1, Q80TR6

Conserved Domains (5) summary

COG1196
Location:250 → 954

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

COG4942
Location:114 → 374

EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:1853 → 2515

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam10495
Location:3589 → 3670

PACT_coil_coil; Pericentrin-AKAP-450 domain of centrosomal targeting protein

pfam17380
Location:1505 → 1690

DUF5401; Family of unknown function (DUF5401)
XM_036164707.1 → XP_036020600.1 A-kinase anchor protein 9 isoform X6

UniProtKB/Swiss-Prot

Q3TH74, Q70FJ1, Q80TR6

Conserved Domains (5) summary

COG1196
Location:250 → 954

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

COG4942
Location:114 → 374

EnvC; Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:1791 → 2502

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam17380
Location:1505 → 1690

DUF5401; Family of unknown function (DUF5401)

pfam10495
Location:3576 → 3657

PACT_coil_coil; Pericentrin-AKAP-450 domain of centrosomal targeting protein
XM_036164710.1 → XP_036020603.1 A-kinase anchor protein 9 isoform X14

Conserved Domains (3) summary

COG1196
Location:1307 → 1668

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:117 → 775

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam10495
Location:1901 → 1982

PACT_coil_coil; Pericentrin-AKAP-450 domain of centrosomal targeting protein