GEO DataSets

Analysis of growth factor-mobilized, CD34+ hematopoietic stem cells in vitro differentiated to day 10 (polychromatophilic stage) or day 14 (orthochromatic stage) then infected with P. falciparum for 24 hr. Results provide insight into host erythroblast cell response to infection by P. falciparum.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 development stage, 2 infection sets

Platform:

GPL570

Series:

GSE24849

16 Samples

Download data: CEL

(Submitter supplied) Global, genomic responses of erythrocytes to infectious agents have been difficult to measure, because these cells are e-nucleated. We have previously demonstrated that in vitro matured, nucleated erythroblast cells at the orthochromatic stage can be efficiently infected by the human malaria parasite Plasmodium falciparum. We now show that infection of orthochromatic cells induces change in 609 host genes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS4557 GDS4558

Platform:

GPL570

28 Samples

Download data: CEL

Analysis of growth factor-mobilized, CD34+ hematopoietic stem cells in vitro differentiated to day 10 (polychromatophilic stage) or day 14 (orthochromatic stage) then infected with P. falciparum for 24 hr. Results provide insight into host erythroblast cell response to infection by P. falciparum.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 development stage, 2 infection sets

Platform:

GPL570

Series:

GSE24849

12 Samples

Download data: CEL

(Submitter supplied) In malaria infection, Plasmodium spp. parasites accumulate in the bone marrow near sites of erythroid development. While it has been observed that Plasmodium falciparum infection of late-stage erythroblasts can delay terminal erythroid differentiation and enucleation, the mechanism(s) underlying this phenomenon are unknown. Here, we apply RNA-seq after fluorescence-activated cell sorting (FACS) of infected erythroblasts to identify transcriptional responses to direct and indirect interaction with P. more...

Organism:

Homo sapiens; Plasmodium falciparum

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL33445 GPL24676

83 Samples

Download data: TXT

(Submitter supplied) Normal erythropoiesis requires a critical balance between proapoptotic and antipaoptotic pathways. Bcl-xl, an antiapoptotic protein is induced at end-stages of differentiation of erythroid precursors in response to erythropoietin. The details of the proapoptotic pathway and the critical proapoptotic proteins inhibited by Bcl-xl in erythropoiesis are not well understood. We employed gene targeting to ablate Nix, a proapoptotic BH3-domain only Bcl2 family protein, which is known to be transcriptionally induced during erythropoiesis. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2630

Platform:

GPL1261

8 Samples

Download data: CEL

Analysis of spleens of mutants lacking Nix, a proapoptotic BH3-only factor that is transcriptionally induced during erythropoiesis. Erythropoiesis requires a critical balance between proapoptotic and antipaoptotic pathways. Results provide insight into the role of Nix in erythropoiesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE7020

8 Samples

Download data: CEL

(Submitter supplied) Cerebral malaria is a severe multifactorial condition associated with the interaction of high numbers of infected erythrocytes to human brain endothelium without invasion into the brain. The result is coma and seizures with death in more than 20% of cases. Because the brain endothelium is at the interface of these processes, we investigated the global gene responses of human brain endothelium after the interaction with Plasmodium falciparum–infected erythrocytes with either high- or low-binding phenotypes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

8 Samples

Download data: CEL