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Series GSE9861 Query DataSets for GSE9861
Status Public on Nov 10, 2009
Title Effect of Plasmodium falciparum infected erythrocytes on primary human brain microvascular endothelial cell
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Cerebral malaria is a severe multifactorial condition associated with the interaction of high numbers of infected erythrocytes to human brain endothelium without invasion into the brain. The result is coma and seizures with death in more than 20% of cases. Because the brain endothelium is at the interface of these processes, we investigated the global gene responses of human brain endothelium after the interaction with Plasmodium falciparum–infected erythrocytes with either high- or low-binding phenotypes. The most significantly up-regulated transcripts were found in gene ontology groups comprising the immune response, apoptosis and antiapoptosis, inflammatory response, cell-cell signaling, and signal transduction and nuclear factor B (NF-B) activation cascade. The proinflammatory NF-B pathway was central to the regulation of the P falciparum–modulated endothelium transcriptome. The proinflammatory molecules, for example, CCL20, CXCL1, CXCL2, IL-6, and IL-8, were increased more than 100-fold, suggesting an important role of blood-brain barrier (BBB) endothelium in the innate defense during P falciparum–infected erythrocyte (Pf-IRBC) sequestration. However, some of these diffusible molecules could have reversible effects on brain tissue and thus on neurologic function. The inflammatory pathways were validated by direct measurement of proteins in brain endothelial supernatants. This study delineates the strong inflammatory component of human brain endothelium contributing to cerebral malaria.
Overall design Total of 8 samples (4 control and 4 treated) were analyzed. 4 control samples included two normal RBC control and two medium controls. 4 treated samples includes 2 exposed to low binding Pf-IRBC and 2 exposed to high binding Pf-IRBC (Pf-IRBC-P). Medium and RBC controls were finally used as four replicates of control and all four Pf-IRBC or Pf-IRBC-P exposed endothelial cells were used as 4 separate treated controls.
Contributor(s) Tripathi AK, Stins MF, Sha W, Shulaev V, Sullivan DJ
Citation(s) 19713460
Submission date Dec 12, 2007
Last update date Dec 06, 2018
Contact name Abhai K Tripathi
Phone 410-614-1562
Fax 410-955-0105
Organization name Johns Hopkins University
Department Mol Microbiol and Immunology, SPH and Neurology, SOM
Lab Sullivan, DJ (MMI) and Stins MF (Neurology)
Street address 615 N Wolfe St
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (8)
GSM249129 Selected 3D7 HBMEC 6hr biol-rep1
GSM249130 Selected 3D7 HBMEC 6hr biol-rep2
GSM249131 Unselected 3D7 HBMEC 6hr biol-rep1
BioProject PRJNA103845

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9861_RAW.tar 14.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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