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| Status |
Public on Jul 25, 2023 |
| Title |
Transcriptional profiling of primary human erythroblasts infected with Plasmodium falciparum |
| Organisms |
Plasmodium falciparum; Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In malaria infection, Plasmodium spp. parasites accumulate in the bone marrow near sites of erythroid development. While it has been observed that Plasmodium falciparum infection of late-stage erythroblasts can delay terminal erythroid differentiation and enucleation, the mechanism(s) underlying this phenomenon are unknown. Here, we apply RNA-seq after fluorescence-activated cell sorting (FACS) of infected erythroblasts to identify transcriptional responses to direct and indirect interaction with P. falciparum.
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| Overall design |
Comparative gene expression profiling analysis of human erythroblasts sorted into populations and analyzed by RNA-seq. Transcriptomic profiles (4-6 biological replicates) were obtained from four cell types (proerythroblast, basophilic erythroblast, polychromatic erythroblast, orthochromatic erythroblast) in four treatment conditions (media-only, dead parasite (SRS, syringe-ruptured schizont), uninfected, infected).
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| Contributor(s) |
Feldman TP, Ryan Y, Egan ES |
| Citation(s) |
37493969 |
| |
| Submission date |
May 30, 2023 |
| Last update date |
Sep 27, 2023 |
| Contact name |
Tamar P Feldman |
| Organization name |
Stanford University
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| Street address |
240 Pasteur Dr
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| City |
Palo Alto |
| State/province |
CA |
| ZIP/Postal code |
94304 |
| Country |
USA |
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| Platforms (2) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
| GPL33445 |
Illumina NovaSeq 6000 (Homo sapiens; Plasmodium falciparum) |
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| Samples (83)
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| GSM7433621 |
ProE, Media, replicate 4 |
| GSM7433622 |
ProE, Media, replicate 5 |
| GSM7433623 |
ProE, Media, replicate 6 |
| GSM7433624 |
ProE, SRS, replicate 1 |
| GSM7433625 |
ProE, SRS, replicate 2 |
| GSM7433626 |
ProE, SRS, replicate 3 |
| GSM7433627 |
ProE, SRS, replicate 4 |
| GSM7433628 |
ProE, SRS, replicate 5 |
| GSM7433629 |
ProE, SRS, replicate 6 |
| GSM7433630 |
ProE, Uninfected, replicate 1 |
| GSM7433631 |
ProE, Uninfected, replicate 2 |
| GSM7433632 |
ProE, Uninfected, replicate 3 |
| GSM7433633 |
ProE, Uninfected, replicate 4 |
| GSM7433634 |
ProE, Uninfected, replicate 5 |
| GSM7433635 |
ProE, Infected, replicate 1 |
| GSM7433636 |
ProE, Infected, replicate 2 |
| GSM7433637 |
ProE, Infected, replicate 3 |
| GSM7433638 |
ProE, Infected, replicate 4 |
| GSM7433639 |
BasoE, Media, replicate 1 |
| GSM7433640 |
BasoE, Media, replicate 2 |
| GSM7433641 |
BasoE, Media, replicate 3 |
| GSM7433642 |
BasoE, Media, replicate 4 |
| GSM7433643 |
BasoE, Media, replicate 5 |
| GSM7433644 |
BasoE, Media, replicate 6 |
| GSM7433645 |
BasoE, SRS, replicate 1 |
| GSM7433646 |
BasoE, SRS, replicate 2 |
| GSM7433647 |
BasoE, SRS, replicate 3 |
| GSM7433648 |
BasoE, SRS, replicate 4 |
| GSM7433649 |
BasoE, SRS, replicate 5 |
| GSM7433650 |
BasoE, SRS, replicate 6 |
| GSM7433651 |
BasoE, Uninfected, replicate 1 |
| GSM7433652 |
BasoE, Uninfected, replicate 2 |
| GSM7433653 |
BasoE, Uninfected, replicate 3 |
| GSM7433654 |
BasoE, Uninfected, replicate 4 |
| GSM7433655 |
BasoE, Infected, replicate 1 |
| GSM7433656 |
BasoE, Infected, replicate 2 |
| GSM7433657 |
BasoE, Infected, replicate 3 |
| GSM7433658 |
BasoE, Infected, replicate 4 |
| GSM7433659 |
PolyE, Media, replicate 1 |
| GSM7433660 |
PolyE, Media, replicate 2 |
| GSM7433661 |
PolyE, Media, replicate 3 |
| GSM7433662 |
PolyE, Media, replicate 4 |
| GSM7433663 |
PolyE, Media, replicate 5 |
| GSM7433664 |
PolyE, SRS, replicate 1 |
| GSM7433665 |
PolyE, SRS, replicate 2 |
| GSM7433666 |
PolyE, SRS, replicate 3 |
| GSM7433667 |
PolyE, SRS, replicate 4 |
| GSM7433668 |
PolyE, SRS, replicate 5 |
| GSM7433669 |
PolyE, SRS, replicate 6 |
| GSM7433670 |
PolyE, Uninfected, replicate 1 |
| GSM7433671 |
PolyE, Uninfected, replicate 2 |
| GSM7433672 |
PolyE, Uninfected, replicate 3 |
| GSM7433673 |
PolyE, Uninfected, replicate 4 |
| GSM7433674 |
PolyE, Uninfected, replicate 5 |
| GSM7433675 |
PolyE, Uninfected, replicate 6 |
| GSM7433676 |
PolyE, Infected, replicate 1 |
| GSM7433677 |
PolyE, Infected, replicate 2 |
| GSM7433678 |
PolyE, Infected, replicate 3 |
| GSM7433679 |
PolyE, Infected, replicate 4 |
| GSM7433680 |
PolyE, Infected, replicate 5 |
| GSM7433681 |
OrthoE, Media, replicate 1 |
| GSM7433682 |
OrthoE, Media, replicate 2 |
| GSM7433683 |
OrthoE, Media, replicate 3 |
| GSM7433684 |
OrthoE, Media, replicate 4 |
| GSM7433685 |
OrthoE, Media, replicate 5 |
| GSM7433686 |
OrthoE, SRS, replicate 1 |
| GSM7433687 |
OrthoE, SRS, replicate 2 |
| GSM7433688 |
OrthoE, SRS, replicate 3 |
| GSM7433689 |
OrthoE, SRS, replicate 4 |
| GSM7433690 |
OrthoE, SRS, replicate 5 |
| GSM7433691 |
OrthoE, SRS, replicate 6 |
| GSM7433692 |
OrthoE, Uninfected, replicate 1 |
| GSM7433693 |
OrthoE, Uninfected, replicate 2 |
| GSM7433694 |
OrthoE, Uninfected, replicate 3 |
| GSM7433695 |
OrthoE, Uninfected, replicate 4 |
| GSM7433696 |
OrthoE, Uninfected, replicate 5 |
| GSM7433697 |
OrthoE, Infected, replicate 1 |
| GSM7433698 |
OrthoE, Infected, replicate 2 |
| GSM7433699 |
OrthoE, Infected, replicate 3 |
| GSM7433700 |
OrthoE, Infected, replicate 4 |
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| Relations |
| BioProject |
PRJNA977678 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE233739_RawGeneCounts.txt.gz |
2.9 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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