GEO DataSets

Analysis of bone marrow-derived, hematopoietic stem cells (HSC) from healthy, hematologically normal young and elderly donors. Aged HSCs are increased in frequency and are less quiescent than young HSCs. Results provide insight into molecular mechanisms underlying the hematopoietic aging phenotype.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 age sets

Platform:

GPL570

Series:

GSE32719

27 Samples

Download data: CEL

(Submitter supplied) In the human hematopoietic system, aging is associated with decreased bone marrow cellularity, decreased adaptive immune system function, and increased incidence of anemia and other hematological disorders and malignancies. Recent studies in mice suggest that changes within the hematopoietic stem cell (HSC) population during aging contribute significantly to the manifestation of these age-associated hematopoietic pathologies. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3942

Platform:

GPL570

27 Samples

Download data: CEL

(Submitter supplied) The adaptor protein Lnk is an important negative regulator of HSC homeostasis and self-renewal. This study aims to investigate the role of Lnk in HSC aging. Here we performed expression profiling of bone marrow CD150+CD48-LSK LT-HSCs from young and old WT and Lnk-/- mice. Results identify select Lnk-mediated pathways with potential involvement in HSC self-renewal and aging.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL13730

14 Samples

Download data: CEL

(Submitter supplied) The hematopoietic stem cell (HSC) compartment is heterogeneous, yet our understanding of the identities of different HSC subtypes is limited. Here we show that platelet integrin CD41 (αIIb), currently thought to only transiently mark fetal HSCs, is expressed on an adult HSC subtype that accumulates with age. CD41+ HSCs were largely quiescent and exhibited myeloerythroid and megakaryocyte gene priming, governed by Gata1, whereas CD41- HSCs were more proliferative and exhibited lymphoid gene priming. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL13912

17 Samples

Download data: TXT

(Submitter supplied) To investigate the role of FoxO transcription factors as mediators of hematopoietic stem cell resistance to oxidative stress. Keywords: Hematopoietic stem cells, myeloid progenitors, oxidative stress, ROS, Affymetrix Mouse Genome 430 2.0

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2720

Platform:

GPL1261

12 Samples

Download data: CEL

Analysis of lineage-negative Sca-1+, c-Kit+ (LSK) cells lacking the Forkhead O transcription factors FoxO1, FoxO3, and FoxO4. The LSK cell population is enriched for hematopoietic stem cells (HSCs). Results provide insight into the role of FoxO family members in hematopoiesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 cell type, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE6623

12 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Methylation profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platforms:

GPL13112 GPL17021

72 Samples

Download data: BED, BW

(Submitter supplied) To identify pathways and processes driving the observed hematopoietic stem cell (HSC) aging-like phenotypes in miR-146a-/- vs. WT, we performed RNA-seq gene expression profiling of Lin- Sca-1+ c-Kit+ (LSK) cells isolated from miR-146a-/- or WT mouse bone marrow (BM). Differential expression analysis and EnrichmentMap network analysis identified cytokine signalling and immune pathways as potential drivers of aging-like alterations in miR-146a-/- HSC proliferation and differentiation.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

2 Samples

Download data: TSV

(Submitter supplied) Aging is associated with significant changes in the hematopoietic system, including increased inflammation, impaired hematopoietic stem cell (HSC) function, and increased incidence of myeloid malignancy. Inflammation of aging (“inflammaging”) has been proposed as a driver of age-related changes in HSC function and myeloid malignancy, but mechanisms linking these phenomena remain poorly defined. Here, we identify loss of miR-146a as driving aging-associated inflammation in AML patients. more...

Organism:

Mus musculus

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL13112

1 Sample

Download data: BW

(Submitter supplied) Aging is associated with significant changes in the hematopoietic system, including increased inflammation, impaired hematopoietic stem cell (HSC) function, and increased incidence of myeloid malignancy. Inflammation of aging (“inflammaging”) has been proposed as a driver of age-related changes in HSC function and myeloid malignancy, but mechanisms linking these phenomena remain poorly defined. Here, we identify loss of miR-146a as driving aging-associated inflammation in AML patients. more...

Organism:

Mus musculus

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL17021

69 Samples

Download data: BED

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL21103 GPL19057

50 Samples

Download data

(Submitter supplied) Genome-wide gene expression pattern of E47 KO versus WT HSCs from primary and secondary recipient mice were analysis using Agilent one-color micro-array analysis.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

8 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17021

10 Samples

Download data: BED, NARROWPEAK

(Submitter supplied) To ensure the rapid response to stimuli, some HSCs are specifically prepared (primed) for tasks involving activation and reconstitution. However, the key factors that regulate the primed state of HSCs are largely unknown. Here we report that Med23 controls the formation of myeloid-primed HSCs. Ablation of Med23 in hematopoietic system leads to lymphocytopenia. Moreover, Med23-deficient HSCs undergo myeloid-biased differentiation and lose the self-renewal capacity. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

6 Samples

Download data: TXT

(Submitter supplied) To ensure the rapid response to stimuli, some HSCs are specifically prepared (primed) for tasks involving activation and reconstitution. However, the key factors that regulate the primed state of HSCs are largely unknown. Here we report that Med23 controls the formation of myeloid-primed HSCs. Ablation of Med23 in hematopoietic system leads to lymphocytopenia. Moreover, Med23-deficient HSCs undergo myeloid-biased differentiation and lose the self-renewal capacity. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17021

4 Samples

Download data: BED, NARROWPEAK

(Submitter supplied) In response to myeloablative stresses, HSCs are rapidly activated to replenish myeloid progenitors, while maintaining full potential of self-renewal to ensure life-long hematopoiesis. However, the key factors that orchestrate HSC activities during physiological stresses remain largely unknown. Here we report that Med23 controls the myeloid potential of activated HSCs. Ablation of Med23 in hematopoietic system leads to lymphocytopenia. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

8 Samples

Download data: TXT, XLSX

(Submitter supplied) Loss of immune function and an increased incidence of myeloid leukemia are two of the most clinically significant consequences of aging of the hematopoietic system. To better understand the mechanisms underlying hematopoietic aging, we evaluated the cell intrinsic functional and molecular properties of highly purified long-term hematopoietic stem cells (LT-HSCs) from young and old mice. We found that LT-HSC aging was accompanied by cell autonomous changes, including increased stem cell self-renewal, differential capacity to generate committed myeloid and lymphoid progenitors, and diminished lymphoid potential. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1803

Platform:

GPL1261

8 Samples

Download data: CEL

Analysis of highly purified long-term hematopoietic stem cells from young and old C57BL/6s at 2 to 3 months and 22 to 24 months of age, respectively. Results provide insight into the cellular and molecular changes underlying hematopoietic stem cell aging.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 age sets

Platform:

GPL1261

Series:

GSE4332

8 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL15103

4 Samples

Download data

(Submitter supplied) RNA-seq in wt and Usp16 deleted HSC/P

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15103

2 Samples

Download data: TXT