GEO DataSets

Analysis of umbilical vein endothelial cells (HUVEC) treated with VEGF-A for up to 6 hours in vitro. VEGF-A is a major trigger of vasculogenesis and physiologic angiogenesis. Results provide insight into the molecular mechanisms underlying the vasculogenic and angiogenic activity of VEGF-A.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 5 time sets

Platform:

GPL97

Series:

GSE10778

5 Samples

Download data: CEL, CHP

(Submitter supplied) Angiogenesis, the formation of new capillaries by sprouting from preexisting vessels, is mainly induced by VEGF-A. To identify genes which are induced by VEGF-A in endothelial cells, HUVEC were starved and induced by VEGF-A165 for 30, 60 and 150min. RNA of induced and uninduced cells was isolated and subjected to microarray analysis using Affymetrix microarray.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3567

Platform:

GPL570

4 Samples

Download data: CEL, CHP

(Submitter supplied) Angiogenesis is defined as the formation of new capillaries by sprouting from preexisting vessels. It is mainly triggered by vascular endothelial growth factor (VEGF) and occurs in the adult primarily in wound healing processes or in pathologic tumor vessel growth. To identify genes specifically triggered by VEGF and involved in the process of angiogenesis, we utilized Affymetrix microarrays hybridized with cRNA of human umbilical vein endothelial cells (HUVEC) stimulated with either the main trigger of angiogenesis, VEGF or a more general mitogenic growth factor, EGF. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS3568 GDS3570

Platforms:

GPL97 GPL96

14 Samples

Download data: CEL, CHP

Analysis of umbilical vein endothelial cells (HUVEC) treated with VEGF-A or EGF for up to 6 hours. VEGF-A is a major trigger of vasculogenesis and angiogenesis. EGF is a general growth factor. Results provide insight into the mechanisms underlying the vasculogenic and angiogenic activity of VEGF-A.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 agent, 5 time sets

Platform:

GPL96

Series:

GSE10778

9 Samples

Download data: CEL, CHP

Analysis of umbilical vein endothelial cells (HUVEC) treated with VEGF-A for up to 150 minutes in vitro. VEGF-A is a major trigger of vasculogenesis and physiologic angiogenesis. Results provide insight into the molecular mechanisms underlying the vasculogenic and angiogenic activity of VEGF-A.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 4 time sets

Platform:

GPL570

Series:

GSE15464

4 Samples

Download data: CEL, CHP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL570 GPL10999

11 Samples

Download data: CEL, WIG

(Submitter supplied) We performed the newly mapping of genome-wide NFATc1 binding events in VEGF-stimulated primary cultured endothelial cells, by chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq). Combined NFATc1 ChIP-seq profile and the epigenetic histone marks revealed that predominant NFATc1-occupied peaks were overlapped with promoter marking but not silencer marking. DNA microarrays with NFATc1 expression or knockdown indicated the predominant NFATc1 binding targets were correlated with induced patterns.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL10999

6 Samples

Download data: WIG

(Submitter supplied) We performed the newly mapping of genome-wide NFATc1 binding events in VEGF-stimulated primary cultured endothelial cells, by chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq). Combined NFATc1 ChIP-seq profile and the epigenetic histone marks revealed that predominant NFATc1-occupied peaks were overlapped with promoter marking but not silencer marking. DNA microarrays with NFATc1 expression or knockdown indicated the predominant NFATc1 binding targets were correlated with induced patterns.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

5 Samples

Download data: CEL

(Submitter supplied) Vascular permeability reflects changes in the function of the endothelium, its interendothelial junctions and transcellular delivery. Here, we show that common molecular mechanisms exist between VEGF and histamine in regulating vascular hyperpermeability. Crosstalk between downstream signaling of VEGF and histamine receptors are involved in calcium signaling and cell proliferation. Understanding the molecular mechanisms of vascular permeability is crucial in order to reduce vascular hyperpermeability and oedema in various pathological conditions and in VEGF therapy.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

6 Samples

Download data: BEDGRAPH, TXT

(Submitter supplied) Nkx2-3 is associated with inflammatory bowel disease (IBD). Nkx2-3 is expressed in microvascular endothelial cells and the muscuularis mucosa of the gastrointestinal tract. Human intestinal microvascular cells (HIMEC) are actively involved in the pathogenesis of IBD and IBD-associated microvascular dysfunction. To understand the cellular function of Nkx2-3 and its potential role underlying IBD pathogenesis, we investigated the genes regulated by Nkx2-3 in HIMEC usin cDNA microarray.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

8 Samples

Download data: CSV, TXT

(Submitter supplied) Angiogenesis in cultures of rat aorta begins with neovessels sprouting from the aortic explant within the first three days of culture. We used microarrys to examine the effects of TNF-alpha on gene expression in both fibrin and collagen gels during the first 48 hours or culture.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL6247

12 Samples

Download data: CEL

(Submitter supplied) Angiogenesis in collagen gel cultures of rat aorta begins with neovessels sprouting from the aortic explant within the first three days of culture. We used microarrays to detail the pattern of gene expression underlying initial 24 hours of growth, prior to the sprouting of visible neovessles, and identified distinct classes of up-regulated genes during this process.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL6247

6 Samples

Download data: CEL

(Submitter supplied) Specific Aims To identify novel transcriptional events associated with angiogenesis in VEGF and Ang-1 stimulated rat aortic rings. Our studies take advantage of the capacity of rat aortic rings to generate new vessels in collagen gels. Rat aortic rings embedded in collagen gel immediately after excision from the animal produce a self-limited angiogenic response under serum-free conditions and in the absence of exogenous stimuli. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Datasets:

GDS1922 GDS1923

Platforms:

GPL341 GPL342

18 Samples

Download data

Analysis of aortic rings treated with angiopoietin-1 (Ang-1) or vascular endothelial growth factor (VEGF). Aortic rings can generate neovessels ex vivo after angiogenic factor stimulation. Results identify transcriptional events associated with angiogenesis in VEGF and Ang-1 stimulated aortic rings.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 3 agent sets

Platform:

GPL342

Series:

GSE3355

8 Samples

Download data

Analysis of aortic rings treated with angiopoietin-1 (Ang-1) or vascular endothelial growth factor (VEGF). Aortic rings can generate neovessels ex vivo after angiogenic factor stimulation. Results identify transcriptional events associated with angiogenesis in VEGF and Ang-1 stimulated aortic rings.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 3 agent sets

Platform:

GPL341

Series:

GSE3355

9 Samples

Download data

(Submitter supplied) We evaluated the effects of vascular endothelial growth factor -B (VEGF-B) and the deletion of its receptor VEGFR1 from endothelial cells on cardiac hypertrophy. Whole-genome transcriptomic analysis showed that overexpression of the ligand had similar effects on the heart transcriptome as deletion of its receptor. Both treatments also induced angiogenesis and mild cardiac hypertrophy. The aim of the study was to identify pathways, which mediate angiogenesis-induced cardiac hypertrophy.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

12 Samples

Download data: TXT

(Submitter supplied) Background: Aldo-keto reductase (AKR) 1C family member 3 (AKR1C3), one of four identified human AKR enzymes, catalyzes steroid, prostaglandin, and xenobiotic metabolism. In the prostate, AKR1C3 is up-regulated in localized and advanced prostate adenocarcinoma, and is associated with prostate cancer (PCa) aggressiveness. Here we provide initial evidence for potential roles of AKR1C3 in PCa progression. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL7363

5 Samples

Download data: TXT

(Submitter supplied) Lymphatic vessel growth and activation, mediated by vascular endothelial growth factor- (VEGF)-C and/or VEGF-A, play an important role in metastatic cancer spread and in chronic inflammation. We aimed to comprehensively identify downstream molecular targets induced by VEGF-A or VEGF-C in lymphatic endothelium. To this end, we treated human dermal lymphatic endothelial cells (LEC) with VEGF-A or VEGF-C for up to 24 hours, followed by a time-series transcriptional profiling using gene microarray technology. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL2986

27 Samples

Download data: TXT