GEO DataSets

Analysis of Saccharomyces cerevisiae cultures at various time points up to 120 minutes following treatment with the oxidant cumene hydroperoxide (CHP). Results provide insight into molecular mechanisms underlying the response of the yeast S. cerevisiae to oxidative stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, transformed count, 2 agent, 8 time sets

Platform:

GPL90

Series:

GSE7645

48 Samples

Download data: CEL

(Submitter supplied) Oxidative stress is a harmful condition in a cell, tissue, or organ, caused by an imbalnace between reactive oxygen species and other oxidants and the capacity of antioxidant defense systems to remove them. The budding yeast S. cerevisiae has been the major eukaryotic model for studies of response to oxidative stress. We used microarrays to study the genome-wide temporal response of the yeast S. cerevisiae to oxidative stress induced by cumene hydroperoxide. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS3035

Platform:

GPL90

48 Samples

Download data: CEL

(Submitter supplied) The Saccharomyces cerevisiae Yap1p transcription factor is required for the H2O2-dependent activation of many antioxidant genes including the TRX2 gene encoding thioredoxin 2. To identify factors that regulate Yap1p activity, we carried out a genetic screen for mutants that show elevated expression of a TRX2-HIS3 fusion in the absence of H2O2. Two independent mutants isolated in this screen carried mutations in the TRR1 gene encoding thioredoxin reductase. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platforms:

GPL2659 GPL2657 GPL2658

15 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13821

66 Samples

Download data: WIG

(Submitter supplied) Snt2 is a yeast chromatin-interacting protein whose function has not been well characterized, that was recently shown to associate with Ecm5 and the Rpd3 deacetylase. Using chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq), we show that in response to H2O2, Snt2 and Ecm5 colocalize to promoters of genes involved in various aspects of the environmental stress response. By integrating these ChIP-seq results with expression analysis, we identify a key set of target genes that require Snt2 for proper expression after H2O2 stress. more...

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13821

30 Samples

Download data: WIG

(Submitter supplied) Snt2 is a yeast chromatin-interacting protein whose function has not been well characterized, that was recently shown to associate with Ecm5 and the Rpd3 deacetylase. Using chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq), we show that in response to H2O2, Snt2 and Ecm5 colocalize to promoters of genes involved in various aspects of the environmental stress response. By integrating these ChIP-seq results with expression analysis, we identify a key set of target genes that require Snt2 for proper expression after H2O2 stress. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13821

18 Samples

Download data: WIG

(Submitter supplied) Snt2 is a yeast chromatin-interacting protein whose function has not been well characterized, that was recently shown to associate with Ecm5 and the Rpd3 deacetylase. Using chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq), we show that in response to H2O2, Snt2 and Ecm5 colocalize to promoters of genes involved in various aspects of the environmental stress response. By integrating these ChIP-seq results with expression analysis, we identify a key set of target genes that require Snt2 for proper expression after H2O2 stress. more...

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13821

18 Samples

Download data: WIG

(Submitter supplied) The Platform data table reflects a condensed representation of the duplicate array features. The full array layout (GPL4873_complete_layout.txt) is linked as a supplementary file at the foot of this record. Protocol: Single stranded (sense) 40mer oligonucleotides were obtained from MWG, diluted and spotted onto glass Eppendorf Creative Oligo Chips using a 24 pin ESI scanner. A total of 13824 features were spotted onto 120 slides within a run where 24 subarrays were added (6x4) with each containing 24 vertical by 24 horizontal features. more...

Organism:

Saccharomyces cerevisiae

1 DataSet

3 Series

30 Samples

Download data: TXT

(Submitter supplied) Cell cycle sensing of oxidative stress in Saccharomyces cerevisiae by oxidation of a specific cysteine residue in the transcription factor Swi6p. Yeast cells begin to bud and enter S phase when growth conditions are favourable during G1 phase. When subjected to oxidative stress, cells arrest at G1 delaying entry into the cell cycle allowing repair of cellular damage. Hence, oxidative stress sensing is coordinated with the regulation of cell cycle. more...

Organism:

Saccharomyces cerevisiae; Schizosaccharomyces pombe

Type:

Expression profiling by array

Platform:

GPL2529

18 Samples

Download data: CEL

(Submitter supplied) Reactive oxygen species, generated in vivo or exogenously encountered, constantly challenge living organisms. Oxidation of polyunsaturated fatty acids (PUFA), which are susceptible to oxidant attack, can lead to initiation of lipid peroxidation and in turn rapid production of toxic lipid hydroperoxides. Eukaryotic microorganisms such as Saccharomyces cerevisiae can survive harsh industrial conditions that contain high levels of the PUFA linoleic acid and its oxidised derivative, linoleic acid hydroperoxide (LoaOOH). more...

Organism:

Schizosaccharomyces pombe; Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL2529

6 Samples

Download data: CEL

(Submitter supplied) We explored genomic expression patterns in the yeast Saccharomyces cerevisiae responding to diverse environmental transitions. DNA microarrays were used to measure changes in transcript levels over time for almost every yeast gene, as cells responded to temperature shocks, hydrogen peroxide, the superoxide-generating drug menadione, the sulfhydryl-oxidizing agent diamide, the disulfide-reducing agent dithiothreitol, hyper- and hypo-osmotic shock, amino acid starvation, nitrogen source depletion, and progression into stationary phase. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Datasets:

GDS15 GDS16 GDS17 GDS18 GDS19 GDS20 GDS21 GDS30 GDS31 GDS33 GDS34 GDS35 GDS36 GDS108 GDS111 GDS112 GDS113 GDS115

9 related Platforms

156 Samples

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Cells grown in minimal medium lacking amino acids and adenine. Samples taken at 0, 0.5, 1, 2, 4 and 6 hours. Included as part of a detailed characterization of global expression programs triggered by environmental stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 5 time sets

Platform:

GPL54

Series:

GSE18

5 Samples

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2.5 mM dithiothrietol (DTT) added to culture and samples collected at several time points up to 480 minutes. Included as part of a detailed characterization of global expression programs triggered by environmental stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 7 time sets

Platform:

GPL54

Series:

GSE18

7 Samples

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Cultures shifted from 30°C to 37°C and samples collected at 0, 5, 15, 30 and 60 minutes. Included as part of a detailed characterization of global expression programs triggered by environmental stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 5 time sets

Platform:

GPL54

Series:

GSE18

5 Samples

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Samples collected from cells grown continuously at 15°C, 17°C, 21°C, 25°C, 29°C, 33°C and 36°C. Included as part of a detailed characterization of global expression programs triggered by stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 7 temperature sets

Platform:

GPL54

Series:

GSE18

8 Samples

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Superoxide-generating drug menadione bisulphate (1 mM) added to culture and samples removed at several time points up to 160 minutes. Included as part of a detailed characterization of global expression programs triggered by environmental stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 9 time sets

Platform:

GPL64

Series:

GSE18

9 Samples

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Heat shock from 29°C to 33°C. Samples collected at 5, 15, 30 and 90 minutes. Included as part of a detailed characterization of global expression programs triggered by environmental stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 4 time sets

Platform:

GPL56

Series:

GSE18

4 Samples

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Heat shock from 29°C to 33°C, with or without addition of 1 M sorbitol. Samples collected at 5, 15 and 30 minutes. Included as part of a detailed characterization of global expression programs triggered by environmental stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 2 agent, 3 time sets

Platform:

GPL56

Series:

GSE18

6 Samples

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Heat shock from 37°C to 25°C. Samples collected at 15, 30, 45, 60 and 90 minutes. Included as part of a detailed characterization of global expression programs triggered by environmental stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 5 time sets

Platform:

GPL56

Series:

GSE18

5 Samples

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Cells resuspended in YPD medium without sorbitol. Samples recovered at 5, 15, 30, 45 and 60 minutes. Included as part of a detailed characterization of global expression programs triggered by stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 5 time sets

Platform:

GPL56

Series:

GSE18

5 Samples

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