GEO DataSets

Analysis of peripheral blood mononuclear cells from C57Bl6 females exposed to a low, intermediate, or lethal dose of total body irradiation. Results used to define gene expression signatures for exposure to different doses of radiation.

Organism:

Mus musculus

Type:

Expression profiling by array, log2 ratio, 4 agent sets

Platform:

GPL4783

Series:

GSE6873

28 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus; Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL4782 GPL4783

106 Samples

Download data: GPR

(Submitter supplied) Gene expression analysis of peripheral blood leukocytes (PB MNCs) to develop expression profiles that accurately reflect prior radiation exposure. Keywords: Comparative, exposure dosage, C57BI6 Murine Irradiation Studies

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2645

Platform:

GPL4783

28 Samples

Download data: GPR

(Submitter supplied) Gene expression analysis of peripheral blood leukocytes (PB MNCs) to develop expression profiles that accurately reflect prior radiation exposure. Keywords: Comparative, exposure dosage, C57BI6 Murine Irradiation Studies, valdiation in human patients

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4782

78 Samples

Download data: GPR

(Submitter supplied) In this study, we asked whether expression profiling could generate a “signature” of a dose of exposure to a toxic in general and to gamma rays in particular. To characterize genes that could potentially “sign” an exposure to irradiation and could allow the estimation of the received dose, we have performed DNA microarray experiments. Responses to gamma rays were analysed by genome-wide expression profiling at different doses in blood leucocytes of c57bl/6 mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL1063 GPL1091

48 Samples

Download data

(Submitter supplied) Previous work has demonstrated the potential for peripheral blood (PB) gene expression profiling for the detection of disease or environmental exposures. We have sought to determine the impact of several variables on the PB gene expression profile of an environmental exposure, ionizing radiation, and to determine the specificity of the PB signature of radiation versus other genotoxic stresses. Keywords: peripheral blood, gene expression study, radiation reponse

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by array

4 related Platforms

335 Samples

Download data: GPR

(Submitter supplied) The present work identified gene expression changes in response to total body irradiation (TBI) in heart, lungs, and liver tissue in Gottingen minipigs. Animals were exposed to 1.7 Gy, 1.9 Gy, 2.1 Gy and 2.3 Gy of TBI and were followed for 45 days. Organ samples were collected at the end of 45 days or sooner if the animal displayed morbidity necessitating euthanasia. RNA was extracted and gene expression profiling studied using Agilent arrays.

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL16571

90 Samples

Download data: TXT, XLSX

(Submitter supplied) The development of a biodosimetric assay based on the combined gene expression analysis of ex vivo-irradiated human peripheral blood (PB) and humans treated with total body irradiation has been developed. Gene expression profile signature of human radiation injury into a rapid and high-throughput chemical ligation-dependent probe amplification assay (CLPA) has been shown to discriminate 6 radiation dose levels in human PB. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

264 Samples

Download data: CEL

(Submitter supplied) Gene expression profiles of peripheral blood samples from C57BL/6 mice exposed with ionizing radiation. We used mice as model animal to study biologial recovery response after radiation damage. Therefore, we obtained gene expression profiles from C57BL/6 mice exposed with various levels of ionizing radiation, including low and high doses and control groups. In order to measure recovery rate, we collected peripheral blood samples at different time durations after the exposure. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL8321

536 Samples

Download data: CEL

(Submitter supplied) After defining a gene expression signature that predicted radiation exposure dose with high accuracy in human peripheral white blood cells irradiated ex vivo, we now demonstrate the predictive power of gene expression signatures in blood from patients undergoing total body irradiation. Using whole genome microarray analysis, we have identified genes that respond to radiation exposure in cancer patients in vivo. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

66 Samples

Download data: TXT

(Submitter supplied) Understanding the possible impact of potential confounding factors is necessary for any approach to radiation biodosimetry. Potential confounding factors have not been fully addressed for gene expression-based biodosimetry approaches, such as we are developing. To begin addressing this need, we have used an ex vivo irradiated peripheral blood cell model to investigate the potential effect of smoking on the global radiation gene expression response, and looked for genes that respond to radiation differently in smokers and non-smokers, and also in males and females. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

95 Samples

Download data: TXT

(Submitter supplied) For victims of radiological accidents, rapid dose estimation and damage prediction are essential. Administering the gold-standard biodosimetry chromosome aberration assay requires highly skilled individuals and several days of labor; consequently, rapid turnaround is an important concern. Identification of new dose estimation markers and damage-predicting in vivo molecules to replace the chromosome aberration assay is crucial to improving the delivery time of medical treatment. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

17 Samples

Download data: CEL

(Submitter supplied) Radiation biodosimetry can play a critical role in the response to a large-scale radiologic emergency, and gene expression profiles have shown promise for providing biodosimetric information. This study was designed to test if gene expression could be used to distinguish between doses received from acute exposures and more protracted exposures, such as those that would result from fallout. Mice were exposed to whole body X-rays at low dose rate (LDR, 3.09 mGy/min) for 6, 12, or 24 hours (1.1, 2.2, or 4.4 Gy), or to equivalent doses delivered at high dose rate (HDR, 1.03 Gy/min). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10333

48 Samples

Download data: TXT

(Submitter supplied) Most studies have analysed the effects of high dose radiation such as atomic bomb survivors in Japan, people exposed during the Chernobyl nuclear accident, patients undergoing radiation therapy, uranium miners, etc. However, it has been difficult to measure and assess the risk of cancer in people exposed to lower doses of ionising radiation, such as the people living at high altitudes, who are exposed to more natural background radiation from cosmic rays than people at sea level. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

24 Samples

Download data: TXT

(Submitter supplied) gene expression host responses to biological threat agents in human PBMC Keywords: pathogen response

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL127

132 Samples

Download data

(Submitter supplied) We conducted a two-phase mouse total-body irradiation (TBI) experiment and measured the RNA m6A levels in peripheral blood mononuclear cells (PBMCs). Phase II

Organism:

Mus musculus

Type:

Other

Platform:

GPL25915

9 Samples

Download data: TXT

(Submitter supplied) We conducted a two-phase mouse total-body irradiation (TBI) experiment and measured the RNA m6A levels in peripheral blood mononuclear cells (PBMCs). Phase I

Organism:

Mus musculus

Type:

Other

Platform:

GPL25915

18 Samples

Download data: TXT

(Submitter supplied) Accumulating data suggest that the biological responses to high and low doses of radiation are qualitatively different, necessitating the direct study of low dose responses. Most such studies have utilized 2-dimensional culture systems, which may not fully represent responses in 3-dimensional tissues. To gain insight into low dose responses in tissue, we have profiled global gene expression in EPI-200, a 3-dimensional tissue model that imitates the structure and function of human epidermis, at 4, 16 and 24 hours after exposure to high (2.5 Gy) and low (0.1 Gy) doses of low LET protons. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

27 Samples

Download data: TXT

(Submitter supplied) Accumulating data suggest that the biological responses to high and low doses of radiation are qualitatively different, necessitating the direct study of low dose responses. Most such studies have utilized 2-dimensional culture systems, which may not fully represent responses in 3-dimensional tissues. To gain insight into low dose responses in tissue, we have profiled global gene expression in EPI-200, a 3-dimensional tissue model from MatTek that imitates the structure and function of human epidermis, at 4, 16 and 24 hours after exposure to high (2.5 Gy) and low (0.1 Gy) doses of low LET protons.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

27 Samples

Download data: TXT