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| Status |
Public on May 11, 2023 |
| Title |
Phase I for N6-methyladenosine RNA modification-based biodosimeters for absorbed ionizing radiation dose estimation |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
We conducted a two-phase mouse total-body irradiation (TBI) experiment and measured the RNA m6A levels in peripheral blood mononuclear cells (PBMCs).
Phase I
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| Overall design |
In the phase I experiment, we focused on screening the RNA transcripts with constantly altered m6A levels during a relatively long period of time post irradiation (TPI) in mouse PBMCs. In this phase, a total of 25 mice exposed to a dose of 6.5 Gy gamma rays were randomly divided into 5 groups for a time-course measurement of the physiological and molecular phenotypes of mice, and 5 sham-exposed mice were used as the control group. The phase II experiment focused on identifying the RNA transcripts that still exhibit significantly altered m6A sites even with a lower dose TBI. In this phase, a total of 10 mice exposed to a dose of 2 Gy gamma rays were randomly divided into 2 groups, and 5 sham-exposed mice were used as the control group.
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| Contributor(s) |
Chen H, Lu Y, Zhou G |
| Citation(s) |
37903783 |
| |
| Submission date |
Feb 15, 2023 |
| Last update date |
Nov 16, 2023 |
| Contact name |
Yiming Lu |
| E-mail(s) |
ylu.phd@gmail.com
|
| Organization name |
State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine
|
| Street address |
Taiping Road
|
| City |
Beijing |
| ZIP/Postal code |
100850 |
| Country |
China |
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| Platforms (1) |
| GPL25915 |
Arraystar Mouse Epitranscriptomic microarray [Agilent-085754] |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA935402 |
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