GEO DataSets

Analysis of monocytes (MCs) treated with M-CSF to induce differentiation into macrophages (MPs), and mature MPs treated with either IFN-gamma and LPS or IL-4 to induce polarization to M1 or M2 cells, respectively. Results provide insight into MC to MP differentiation and polarized activation.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 5 development stage sets

Platform:

GPL97

Series:

GSE5099

15 Samples

Download data: CEL

(Submitter supplied) Monocytes were induced to mature to macrophages with M-CSF. Cells were then activated with Interferon gamma and LPS or IL-4.

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS2429 GDS2430

Platforms:

GPL97 GPL96

30 Samples

Download data: CEL

Analysis of monocytes (MCs) treated with M-CSF to induce differentiation into macrophages (MPs), and mature MPs treated with either IFN-gamma and LPS or IL-4 to induce polarization to M1 or M2 cells, respectively. Results provide insight into MC to MP differentiation and polarized activation.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 5 development stage sets

Platform:

GPL96

Series:

GSE5099

15 Samples

Download data: CEL

(Submitter supplied) Human blood monocytes were differentiated over six days with either 100 ng/ml M-CSF or 1 umol/l CXCL4 In atherosclerotic arteries, blood monocytes differentiate to macrophages in the presence of growth factors like macrophage colony-stimulation factor (MCSF) and chemokines like platelet factor 4 (CXCL4). To compare the gene expression signature of CXCL4-induced macrophages with MCSF-induced macrophages or macrophages polarized with IFN-γ/LPS (M1) or IL-4 (M2), we cultured primary human peripheral blood monocytes for six days. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3787

Platform:

GPL570

4 Samples

Download data: CEL

Analysis of blood monocytes differentiated to macrophages (MP) with CXC Chemokine Ligand 4 (CXCL4) and macrophage colony-stimulation factor (M-CSF). In atherosclerotic arteries, monocytes differentiate to MPs in the presence of growth factors, such as M-CSF, and chemokines, such as CXCL4.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL570

Series:

GSE20484

4 Samples

Download data: CEL

(Submitter supplied) Human cytomegalovirus induces a pro-inflammatory monocyte following infection. To begin to address how HCMV induces these rapid changes in infected monocytes, we examined the transcriptome of infected monocytes. Global transcriptional profiling using cDNA microarrays revealed a significant number of pro-inflammatory genes were upregulated within 4 hours post infection. Keywords: Disease State

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL8300

12 Samples

Download data: CEL, CHP

(Submitter supplied) Monocytes cultured in media containing GM-CSF and IL4 for 5 days normally differentiate into immature dendritic cells which, upon further stimulation with LPS for an additional 2 days, acquire a mature phenotype. In the present study, we evaluated the effect of human cord-blood mesenchymal stem cells (MSC) on human monocyte differentiation into immature and mature DCs by examining the trancriptional profile of the cells that were obtained after both the differentiation and maturation stages.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

18 Samples

Download data: TXT

(Submitter supplied) Identification of genes differentially expressed between human monocyte-macrophages generated in the presence of either GM-CSF (termed M1) or M-CSF (termed M2)

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL11010

6 Samples

Download data: TXT

(Submitter supplied) In the present study we aimed at producing homogeneous populations of monocytes and macrophages from human embryonic stem (hES) cells. Human embryonic stem cell lines KCL001, KCL002, and HUES-2 were differentiated into monocytes by coculture-free differentiation with two growth factors using a three-step method. The method involved embryoid body (EB) formation in hES media, directed differentiation with macrophage colony-stimulating factor and interleukin (IL)-3, and harvest of nonadherent monocytes from the culture supernatants. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

9 Samples

Download data: CEL

(Submitter supplied) Macrophage polarization between the M2 (repair, pro-tumorigenic) and M1 (inflammatory) phenotypes is seen as a continuum of states. The detailed transcriptional events and signals downstream of CSF-1R that contribute to amplification of the M2 phenotype and suppression of the M1 phenotype are largely unknown. Macrophage CSF-1R pTyr-721 signaling promotes cell motility and enhancement of tumor cell invasion in vitro. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

32 Samples

Download data: CEL

(Submitter supplied) Macrophages are able to differentiate into classically polarized (M1) or alternatively polarized (M2) states upon encountering pro-inflammatory cytokines such as interferon (IFN) g or anti-inflammatory cytokines such as interleukin (IL) -4/IL-13, respectively. Moreover, macrophages are known to regulate lipid metabolism via multiple members of the nuclear hormone receptor family, including the retinoid X receptors (RXR). more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:

GPL16791

18 Samples

Download data: TXT

(Submitter supplied) To examine mononuclear cell gene expression profiles in patients with and without SLE and subsets with and without atherosclerosis

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

72 Samples

Download data: TXT

(Submitter supplied) Macrophages play a critical role in the pathogenesis of many diseases, including rheumatoid arthritis, inflammatory bowel disease and atherosclerosis. Monocytes recruited into tissues from peripheral blood differentiate into macrophages. There is limited data concerning the global changes in the expression of genes during monocyte to macrophage, and how the patterns of change identify the mechanism contributing to differentiation or macrophage function. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3203

Platform:

GPL96

9 Samples

Download data: CEL

Analysis of monocytes following up to 168 hours of adherence-induced differentiation. Monocytes differentiate into macrophages, which are critical in the pathogenesis of many diseases. Results provide insight into the molecular mechanisms underlying monocyte to macrophage differentiation.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 cell type, 3 time sets

Platform:

GPL96

Series:

GSE8286

9 Samples

Download data: CEL

(Submitter supplied) Using the highly sensitive miRNA microarray, we screened 107 up-regulated microRNAs and 33 down-regulated microRNAs in the INT-HA-induced M2-like macrophages and we explored the functions of these miRNAs in macrophage polarization. The enrichment results indicated that these miRNAs might participate in the process of macrophage polarization. Furthermore, the quantitative real-time polymerase chain reaction results showed that miR-935 may play an important role in M2 macrophage polarization which was activated by INT-HA.

Organism:

Human immunodeficiency virus 1; Human betaherpesvirus 6B; Homo sapiens; Betapolyomavirus hominis; Herpesvirus saimiri (strain 11); JC polyomavirus; Betapolyomavirus macacae; Human alphaherpesvirus 2; Merkel cell polyomavirus

Type:

Non-coding RNA profiling by array

Platform:

GPL24594

6 Samples

Download data: GPR

(Submitter supplied) Inflammatory cytokine responses to activation of innate immunity differ between individuals, yet the genomic and transcriptomic determinants of inflammatory responsiveness are not well understood. We hypothesized that mRNA and lincRNA expression is modulated in disease-relevant tissues in humans in vivo during inflammation, and differs between individuals with divergent evoked inflammatory responses. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

30 Samples

Download data: DIFF

(Submitter supplied) We compare, in human macrophages, stimuli considered to induce M1 or M2 type of activation, also termed classical and alternative activation. This dataset highlights key differences between the M1 and M2 stimuli pointing to the need of a revision of the paradigm. Macrophages were left untreated or stimulated with IL-4 and IL-13 (prototypic M1 stimuli), Dexamethasone (Glucocorticoid receptor agonist) which has been hitherto considered M2, IL-10 which also falls under the M2 umbrella, and LPS and Interferon Gamma considered M1 stimuli. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

7 Samples

Download data: TXT

(Submitter supplied) The human AA dataset 5 includes the gene expression profile of monocyte-derived macrophages isolated from blood donor buffy coats and plated on TCP in medium containing 10% autologous serum. Cells were stimulated for 120 hours with 50 ng/ml of IL-4, 50 U/ml of IFN-γ plus 12.5 ng/ml of TNF-α, 50 ng/ml of M-CSF or 50 ng/ml of IL-10. RNA samples were isolated using TriPure isolation reagent (Roche) and the RNeasy Mini Kit-Qiagen Clean up method. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

12 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus; Homo sapiens

Type:

Expression profiling by array

5 related Platforms

73 Samples

Download data: CEL

(Submitter supplied) Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from Thioglycollate and Biogel elicited peritoneal macrophages exposed to 20 ng/ml of IL-4 for 18 hours.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL