GEO DataSets

(Submitter supplied) Gene transcription effects of mutations in the infuenza virus A/Hong Kong/1/1968(H3N2) nonstructural 1 NS1 gene in infected human A549 (lung epithilium) cells Influenza A/Hong Kong/156/1997(H5N1) virus NS1 gene mutations F103L and M106I both increase IFN antagonism, virulence and cytoplasmic localization but differ in binding to RIG-I and CPSF30 (manuscript submitted to Virology Journal). Human cells were infected with influenza viruses mutants with specific gain of function mutations in the NS1 gene in order to assess the affects of each mutation on host gene expression. more...

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by array

Datasets:

GDS4997 GDS4998

Platforms:

GPL6246 GPL6244

36 Samples

Download data: CEL, CHP

Analysis of M1 cells infected with H3N2 influenza isolate A/Hong Kong/1/1968 (HK-wt) or recombinant HK NS1 mutants. NS1 is a multifunctional virulence factor. These results, together with those from GDS4997, provide insight into molecular effects of NS1 mutation on mouse versus human host cells.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 6 infection sets

Platform:

GPL6246

Series:

GSE48200

18 Samples

Download data: CEL, CHP

Analysis of A549 cells infected with H3N2 influenza isolate A/Hong Kong/1/1968 (HK-wt) or recombinant HK NS1 mutants. NS1 is a multifunctional virulence factor. These results, together with those from GDS4998, provide insight into molecular effects of NS1 mutation on human versus mouse host cells.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 6 infection sets

Platform:

GPL6244

Series:

GSE48200

18 Samples

Download data: CEL, CHP

(Submitter supplied) The NS1 protein of influenza A virus (IAV) is a multifunctional virulence factor. Mouse adaptive mutations in the NS1 protein of the human isolate A/Hong Kong/1/1968(H3N2) (HK) have been previously reported to increase virulence, viral fitness, and interferon antagonism, but differ in binding to post-transcriptional processing factor CPSF30. Because nuclear trafficking is a major genetic determinant of influenza virus host adaptation, we assessed subcellular localization and host gene expression of NS1 adaptive mutations. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4996

Platform:

GPL6246

42 Samples

Download data: CEL, CHP

Analysis of M1 cells following infection with H3N2 human influenza isolate A/Hong Kong/1/1968 (HK-wt) or mouse-adapted (MA), recombinant HK NS1 mutants. NS1 protein is a multifunctional virulence factor. Results provide insight into the molecular effects of MA NS1 mutations on host gene expression.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 14 infection sets

Platform:

GPL6246

Series:

GSE48217

42 Samples

Download data: CEL, CHP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

18 Samples

Download data: CEL

(Submitter supplied) Recently, a novel protein in the influenza virus segment 3 has been identified, namely PA-X. This small protein has been reported to play a role in modulating host response of the 1918 H1N1 pandemic virus-infected mice. However, poteinal role of this protein in the pathogenicity and regulating host response of the highly pathogenic H5N1 virus in a chicken animal model is completely unknown. We used microarray analysis to evaluate the global transcriptional response in the lungs of the chickens infected with the parental strain (CK10) and PA-X deficiency mutant strain (CK-PAX3).

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

9 Samples

Download data: CEL

(Submitter supplied) Recently, a novel protein in the influenza virus segment 3 has been identified, namely PA-X. This small protein has been reported to play a role in modulating host response of the 1918 H1N1 pandemic virus-infected mice. However, poteinal role of this protein in the pathogenicity and regulating host response of the highly pathogenic H5N1 virus in a chicken animal model is completely unknown. We used microarray analysis to evaluate the global transcriptional response in the lungs of the chickens infected with the parental strain (CK10) and PA-X deficiency mutant strain (CK-PAX3).

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

9 Samples

Download data: CEL

(Submitter supplied) Transcriptional profiling of Human Embryonic Kidney(293T) Cells comparing control untreated 293T cells with 293T cells transfected with A) pcDNA 3.0(-) vector[Invitrogen] (Mock) and B) Expression vector pcDNA 3.0(-) containing cloned Influenza virus H5N1and H11N1-NS1 (Non-Structural1) gene.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL15774

9 Samples

Download data: CSV

(Submitter supplied) The highly pathogenic avian influenza (HPAI) H5N1 viruses continue to circulate in nature and threaten public health. Although several viral determinants and host factors that influence the virulence of HPAI H5N1 viruses in mammals have been identified, the detailed molecular mechanism remains poorly defined and requires further clarification. In our previous studies, we characterized two naturally isolated HPAI H5N1 viruses that had similar viral genomes but differed substantially in their lethality in mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

9 Samples

Download data: TXT