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| Status |
Public on Jul 14, 2014 |
| Title |
Human Embryonic Kidney(293T) Cells: Control vs. Transfected |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptional profiling of Human Embryonic Kidney(293T) Cells comparing control untreated 293T cells with 293T cells transfected with A) pcDNA 3.0(-) vector[Invitrogen] (Mock) and B) Expression vector pcDNA 3.0(-) containing cloned Influenza virus H5N1and H11N1-NS1 (Non-Structural1) gene.
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| Overall design |
Two-condition experiment, 293T vs. TF-293T cells. Biological replicates: 3 control, 3 transfected, independently grown and harvested for each set of experiment.Total 4 groups 1.Control untransfected (293T) cells 2. Mock 293T cells transfected with pcDNA3.0(-) expression 3. 293T cells transfected with NS1 gene of H5N1 virus cloned in pcDNA 3.0(-) vector 4.293T cells transfected with NS1 gene of H11N1 virus cloned in pcDNA 3.0(-) vector.
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| Contributor(s) |
Mukherjee S, Chakrabarti AK |
| Citation(s) |
22866982 |
| |
| Submission date |
Jul 06, 2012 |
| Last update date |
Jul 15, 2014 |
| Contact name |
Sanjay Mukherjee |
| Organization name |
University of Pune
|
| Department |
Department of Zoology
|
| Lab |
Molecular Biology research Lab.
|
| Street address |
Ganeshkhind
|
| City |
Pune |
| State/province |
Maharashtra |
| ZIP/Postal code |
411007 |
| Country |
India |
| |
|
| Platforms (1) |
| GPL15774 |
Human Discover chips oligo array |
|
| Samples (9)
|
| GSM957086 |
H5N1-NS1-Transfected Embryonic kidney (293T) Cells Replicate 1 |
| GSM957087 |
H5N1-NS1-Transfected Embryonic kidney (293T) Cells Replicate 2 |
| GSM957088 |
H5N1-NS1-Transfected Embryonic kidney (293T) Cells Replicate 3 |
| GSM957089 |
H11N1-NS1-Transfected Embryonic kidney (293T) Cells Replicate 1 |
| GSM957090 |
H11N1-NS1-Transfected Embryonic kidney (293T) Cells Replicate 2 |
| GSM957091 |
H11N1-NS1-Transfected Embryonic kidney (293T) Cells Replicate 3 |
| GSM957092 |
pcDNA3.0(-) transfected Embryonic kidney(293T) cells Replicate 1 |
| GSM957093 |
pcDNA3.0(-) transfected Embryonic kidney(293T) cells Replicate 2 |
| GSM957094 |
pcDNA3.0(-) transfected Embryonic kidney(293T) cells Replicate 3 |
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| Relations |
| BioProject |
PRJNA170181 |
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