GEO DataSets

(Submitter supplied) Accurate chromosome segregation requires that sister kinetochores biorient and attach to microtubules from opposite poles. Kinetochore biorientation relies on the underlying centromeric chromatin, which provides a platform to assemble the kinetochore and to recruit the regulatory factors that ensure the high fidelity of this process. To identify the centromeric chromatin determinants that contribute to chromosome segregation, we performed two complementary unbiased genetic screens using a library of mutants in every residue of histone H3 and H4. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL11232

22 Samples

Download data: TXT

(Submitter supplied) Accurate chromosome segregation requires centromeres (CENs), the chromosomal sites where kinetochores form, to bridge DNA and attach to microtubules. In contrast to most eukaryotes, Saccharomyces cerevisiae possesses sequence-defined point centromeres. Chromatin immunoprecipitation followed by sequencing (ChIP-Seq) of four kinetochore components reveals regions of overlapping, extra-centromeric protein localization upon overproduction of the centromeric histone, Cse4 (CENP-A or CenH3). more...

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9377

13 Samples

Download data: BED, SGR, TXT

(Submitter supplied) In order to take an unbiased approach and discover all the locations of Cse4 in the genome, we utilized formaldehyde crosslinking and immunoprecipitation followed by hybridization to DNA microarrays. We analyzed the location of Cse4 in three different strains; one in which the endogenous Cse4 is tagged with 12Myc epitopes (Cse4-12Myc), and one which contained both the endogenous Cse4 (untagged) and an ectopic copy of Cse4-12Myc expressed from the GAL1-10 promoter (pGAL1-10-Cse4-12Myc), and one in which Cse4 is tagged with 3HA epitopes (Cse4-3HA). more...

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL8956

6 Samples

Download data: GPR

(Submitter supplied) Paired-end sequencing study of nucleosomal DNA prepared from budding yeast by micrococcal nuclease digestion.

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9377

4 Samples

Download data: TXT

(Submitter supplied) We report the genome-wide localization of Sgo1p in mitosis of Saccharomyces cerevisiae using ChIP-seq. The high resolution mapping clearly shows a tripartite domain of Sgo1p in each mitotic chromosome. This domain requires the wildtype tension sensing motif (TSM) of histone H3.

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17342

16 Samples

Download data: BEDGRAPH

(Submitter supplied) We mapped ssDNA formation in WT and hpr1delta cells at permissive (30C) and non-permissive (37C) temperature. We showed that hpr1 cells accumulate ssDNA show elevated levels of ssDNA at centromeric regions. Additionally, we show replication fork-associated ssDNA at origins of replication is more confined to the origins in hpr1 cells than WT cells, suggesting a defect in replication fork progression.

Organism:

Saccharomyces cerevisiae

Type:

Other

Platform:

GPL10930

8 Samples

Download data: TXT, XLSX

(Submitter supplied) ChIP-seq profile of Rec8 binding sites arrested in metaphase I by depletion of the APC/C activator CDC20. The aim of this experiment is to identify Rec8 binding sites in metaphase I.

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL22715

2 Samples

Download data: BW

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL22715

10 Samples

Download data: BW

(Submitter supplied) ChIP-seq profile of Spo13 binding sites arrested in prophase I by deletion of the meiotic transcription factor NDT80. Previous ChIP-ChIP data had indicated that Spo13 binding correlates with cohesin binding sites. Therefore, in addition to wild type cells, we also investigated cells deficient of the meiosis-specific cohesin subunit Rec8 to identify cohesin-dependent binding sites of Spo13.

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL22715

4 Samples

Download data: BW

(Submitter supplied) ChIP-seq profile of Sgo1 binding sites arrested in metaphase I by deletion of the APC/C activator CDC20. The aim of this experiment is to identify whether Sgo1 binding differs in wild type cells and cells deficient of the meiosis I-specific protein Spo13, which is required for retention of pericentromeric cohesin after metaphase I.

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL22715

4 Samples

Download data: BW

(Submitter supplied) The segregation of maternal centromeres away from the paternal ones during the first division of meiosis depends on the attachment of sister kinetochores to microtubules emanating from the same spindle pole. In budding yeast monopolar attachment requires the recruitment to kinetochores of a protein complex called monopolin. The biochemical function of monopolin was unknown. Here, we have identified the casein kinase I Hrr25 as a hitherto unknown subunit of monopolin. more...

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL347

5 Samples

Download data: CEL

(Submitter supplied) To protect against aneuploidy, chromosomes must attach to microtubules from opposite poles (“biorientation”) prior to their segregation during mitosis. Biorientation relies on the correction of erroneous attachments by the aurora B kinase, which destabilizes kinetochore-microtubule attachments that lack tension. Incorrect attachments are also avoided because sister kinetochores are intrinsically biased towards capture by microtubules from opposite poles. more...

Organism:

Saccharomyces cerevisiae W303

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL18139

8 Samples

Download data: BEDGRAPH

(Submitter supplied) High resolution ChIP-seq mapping, supported by in vitro reconstitution studies, suggests that the Cse4 nucleosome is a hemisome that occupies the ~80-bp AT-rich CDEII sequence.

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17342

17 Samples

Download data: BED, TXT, WIG

(Submitter supplied) Comparison of genome binding/occupancy profiling of the cohesin subunit Rec8 by high throughput sequencing in WT and ctf19-9A strains in meiotic prophase I.

Organism:

Saccharomyces cerevisiae

Type:

Other

Platform:

GPL27812

6 Samples

Download data: BW, TXT

(Submitter supplied) Kinetochores assemble on centromeres via histone H3 variant CENP-A and low levels of noncoding centromere transcripts (cenRNAs). The latter are ensured by downregulation of RNA polymerase II (RNAPII) and turnover by the nuclear exosome. Using S. cerevisiae, we now add kinase Rio1 to this scheme. Yeast cenRNAs are produced in very low amounts either as short (median lengths of 231nt) or long (4,458nt) transcripts, in a 1:1 ratio. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27812

6 Samples

Download data: TXT

(Submitter supplied) RNA-Seq analysis of S. cerevisiae to study the biology of centromere and pericentromere transcripts.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21656

4 Samples

Download data: TXT

(Submitter supplied) Genome binding/occupancy profiling of the cohesin subunit Scc1 by high throughput sequencing. S. pombe is included as the calibration genome.

Organism:

Saccharomyces cerevisiae; Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL26296

10 Samples

Download data: BW

(Submitter supplied) Genome binding/occupancy profiling of Sgo1 and the condensin subunit Brn1 by high throughput sequencing. S. pombe is included as the calibration genome.

Organism:

Saccharomyces cerevisiae; Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL26296

8 Samples

Download data: BW

(Submitter supplied) Genome binding/occupancy profiling of Sgo1 and the condensin subunit Brn1 by high throughput sequencing. S. pombe is included as the calibration genome.

Organism:

Saccharomyces cerevisiae; Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL26296

12 Samples

Download data: BW

(Submitter supplied) Genome binding/occupancy profiling of the cohesin subunit Scc1 by high throughput sequencing. S. pombe is included as the calibration genome.

Organism:

Saccharomyces cerevisiae; Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL26296

4 Samples

Download data: BW