GEO DataSets

(Submitter supplied) Transcriptional profiling of Human Embryonic Kidney(293T) Cells comparing control untreated 293T cells with 293T cells transfected with A) pcDNA 3.0(-) vector[Invitrogen] (Mock) and B) Expression vector pcDNA 3.0(-) containing cloned Influenza virus H5N1and H11N1-NS1 (Non-Structural1) gene.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL15774

9 Samples

Download data: CSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

18 Samples

Download data: CEL

(Submitter supplied) Recently, a novel protein in the influenza virus segment 3 has been identified, namely PA-X. This small protein has been reported to play a role in modulating host response of the 1918 H1N1 pandemic virus-infected mice. However, poteinal role of this protein in the pathogenicity and regulating host response of the highly pathogenic H5N1 virus in a chicken animal model is completely unknown. We used microarray analysis to evaluate the global transcriptional response in the lungs of the chickens infected with the parental strain (CK10) and PA-X deficiency mutant strain (CK-PAX3).

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

9 Samples

Download data: CEL

(Submitter supplied) Recently, a novel protein in the influenza virus segment 3 has been identified, namely PA-X. This small protein has been reported to play a role in modulating host response of the 1918 H1N1 pandemic virus-infected mice. However, poteinal role of this protein in the pathogenicity and regulating host response of the highly pathogenic H5N1 virus in a chicken animal model is completely unknown. We used microarray analysis to evaluate the global transcriptional response in the lungs of the chickens infected with the parental strain (CK10) and PA-X deficiency mutant strain (CK-PAX3).

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

9 Samples

Download data: CEL

(Submitter supplied) Gene transcription effects of mutations in the infuenza virus A/Hong Kong/1/1968(H3N2) nonstructural 1 NS1 gene in infected human A549 (lung epithilium) cells Influenza A/Hong Kong/156/1997(H5N1) virus NS1 gene mutations F103L and M106I both increase IFN antagonism, virulence and cytoplasmic localization but differ in binding to RIG-I and CPSF30 (manuscript submitted to Virology Journal). Human cells were infected with influenza viruses mutants with specific gain of function mutations in the NS1 gene in order to assess the affects of each mutation on host gene expression. more...

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by array

Datasets:

GDS4997 GDS4998

Platforms:

GPL6246 GPL6244

36 Samples

Download data: CEL, CHP

Analysis of M1 cells infected with H3N2 influenza isolate A/Hong Kong/1/1968 (HK-wt) or recombinant HK NS1 mutants. NS1 is a multifunctional virulence factor. These results, together with those from GDS4997, provide insight into molecular effects of NS1 mutation on mouse versus human host cells.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 6 infection sets

Platform:

GPL6246

Series:

GSE48200

18 Samples

Download data: CEL, CHP

Analysis of A549 cells infected with H3N2 influenza isolate A/Hong Kong/1/1968 (HK-wt) or recombinant HK NS1 mutants. NS1 is a multifunctional virulence factor. These results, together with those from GDS4998, provide insight into molecular effects of NS1 mutation on human versus mouse host cells.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 6 infection sets

Platform:

GPL6244

Series:

GSE48200

18 Samples

Download data: CEL, CHP

(Submitter supplied) While infection of chickens with highly pathogenic avian influenza (HPAI) H5N1 subtypes often leads to complete mortality within 24 to 48 h, infection of ducks in contrast causes mild or no clinical signs. Rapid onsets of fatal disease in chickens, but with no evidence of severe clinical symptoms in ducks, suggest underlying differences in their innate immune mechanisms. To understand the molecular basis for such difference, chicken and duck primary lung cells, infected with a low-pathogenicity avian influenza (LPAI) and two HPAI H5N1 viruses, were subjected to RNA expression profiling using Affymetrix Chicken GeneChip arrays. more...

Organism:

Gallus gallus; Anas platyrhynchos

Type:

Expression profiling by array

Platform:

GPL3213

16 Samples

Download data: CEL, CHP

(Submitter supplied) The NS1 protein of influenza A virus (IAV) is a multifunctional virulence factor. Mouse adaptive mutations in the NS1 protein of the human isolate A/Hong Kong/1/1968(H3N2) (HK) have been previously reported to increase virulence, viral fitness, and interferon antagonism, but differ in binding to post-transcriptional processing factor CPSF30. Because nuclear trafficking is a major genetic determinant of influenza virus host adaptation, we assessed subcellular localization and host gene expression of NS1 adaptive mutations. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4996

Platform:

GPL6246

42 Samples

Download data: CEL, CHP

Analysis of M1 cells following infection with H3N2 human influenza isolate A/Hong Kong/1/1968 (HK-wt) or mouse-adapted (MA), recombinant HK NS1 mutants. NS1 protein is a multifunctional virulence factor. Results provide insight into the molecular effects of MA NS1 mutations on host gene expression.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 14 infection sets

Platform:

GPL6246

Series:

GSE48217

42 Samples

Download data: CEL, CHP

(Submitter supplied) Viral infection is commonly associated with virus-driven hijacking of host proteins. We describe a novel mechanism by which influenza virus impacts host cells through the interaction of influenza NS1 protein with the infected cell epigenome. We show that the NS1 protein of influenza A H3N2 target the transcription elongation PAF1 complex (hPAF1C). We demonstrate that binding of NS1 to hPAF1C results in suppression of hPAF1C-mediated transcriptional elongation. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL10999 GPL11154

17 Samples

Download data: BED

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL10999 GPL10558 GPL11154

89 Samples

Download data: BED

(Submitter supplied) Viral infection is commonly associated with virus-driven hijacking of host proteins. We describe a novel mechanism by which influenza virus impacts host cells through the interaction of influenza NS1 protein with the infected cell epigenome. We show that the NS1 protein of influenza A H3N2 targets the transcription elongation PAF1 complex (hPAF1C). We demonstrate that binding of NS1 to hPAF1C results in suppression of hPAF1C-mediated transcriptional elongation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

36 Samples

Download data: TXT

(Submitter supplied) Viral infection is commonly associated with virus-driven hijacking of host proteins. We describe a novel mechanism by which influenza virus impacts host cells through the interaction of influenza NS1 protein with the infected cell epigenome. We show that the NS1 protein of influenza A H3N2 targets the transcription elongation PAF1 complex (hPAF1C). We demonstrate that binding of NS1 to hPAF1C results in suppression of hPAF1C-mediated transcriptional elongation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

27 Samples

Download data: TXT

(Submitter supplied) Viral infection is commonly associated with virus-driven hijacking of host proteins. We describe a novel mechanism by which influenza virus impacts host cells through the interaction of influenza NS1 protein with the infected cell epigenome. We show that the NS1 protein of influenza A H3N2 targets the transcription elongation PAF1 complex (hPAF1C). We demonstrate that binding of NS1 to hPAF1C results in suppression of hPAF1C-mediated transcriptional elongation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

9 Samples

Download data: TXT

(Submitter supplied) Classical antiviral therapy inhibit viral proteins and are subject to resistance. To counteract this emergence, alternative strategy has been developed that target cellular factors. We hypothesized that such approach could also be useful to identify broad antivirals. Influenza A virus was used as a model for viral diversity and need for therapy against unpredictable viruses as recently underlined by the H1N1 pandemic. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10536

30 Samples

Download data: TXT