GEO DataSets

(Submitter supplied) Atherosclerosis is a transmural chronic inflammatory condition of small and large arteries that is associated with adaptive immune responses at all disease stages. However, impacts of adaptive immune reactions on clinically apparent atherosclerosis such as intima lesion (plaque) rupture, thrombosis, myocardial infarction, and aneurysm largely remain to be identified. It is increasingly recognized that leukocyte infiltrates in plaque, media, and adventitia are distinct but their specific roles have not been defined. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL8321

19 Samples

Download data: CEL

(Submitter supplied) Cultured mouse aorta endothelial cells (from 8-12 weeks old C57BL/6J mice, passage 2-3) were exposed to phosphate buffered saline (control) or a combination of TNFalpha plus agonistic alpha-LTßR antibody for 24 hours as described in Lötzer et al. 2009. Arterioscler. Thromb. Vasc. Biol., in press. Total RNA was extracted and microarrays were prepared.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3810

Platform:

GPL1261

5 Samples

Download data: CEL

(Submitter supplied) Mouse aorta smooth muscle cells (SMCs) express TNF receptor superfamily member 1A (TNFR1) and lymphotoxin ß receptor (LTßR). Circumstantial evidence has linked the SMC LTßR to tertiary lymphoid organogenesis in diseased aortae of hyperlipidemic mice. Here, we explored potential roles of TNFR1 and LTßR activation in cultured SMCs. TNFR1 signaling by TNF activated the classical RelA NF-κB pathway, whereas LTßR signaling by agonistic anti LTßR antibody activated both the classical RelA and alternative RelB NF-κB pathways. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3809

Platform:

GPL1261

21 Samples

Download data: CEL

(Submitter supplied) We previously observed that formation of aorta and innominate artery atherosclerotic lesions in the intima of hyperlipidemic apoE-deficient mice but not wild-type mice was accompanied by a marked age-dependent adventitial T cell infiltration. As the mice aged, adventitial T cells formed T/T cell-, T/B cell-, and T/B/dendritic cell aggregates adjacent to atherosclerotic lesions. Some of the adventitial infiltrates formed large clusters of various immune cells including T cells, B cells (centrocytes, follicular mantle cells), dendritic cells, follicular dendritic cells, and plasma cells with preferential formation in the suprarenal portion of the abdominal aorta. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL8321 GPL1261

35 Samples

Download data: CEL, EXP

(Submitter supplied) Cysteinyl leukotrienes (cysLT), i.e. LTC4, LTD4, and LTE4, are lipid mediators derived from the 5-lipoxygenase pathway. The cysLT receptors cysLT1-R and cysLT2-R are expressed on different target cells and mediate inflammatory reactions in tissue- and LT-R-specific ways. Though endothelial cells (ECs) predominantly express cysLT2-Rs, their role in vascular biology remains to be defined. To delineate cysLT2-R´s action, we stimulated human umbilical vein EC with 100 nM LTD4 for 60 min, determined gene signatures by microarrays, and characterized the resulting EC phenotypes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1926

Platform:

GPL96

21 Samples

Download data

Analysis of cultured aortic endothelial cells stimulated with a combination of tumor necrosis factor (TNF) and α-LTßR antibody for 24 hours. Unlike similarly-treated aortic smooth muscle cells (SMCs), the endothelial cells did not differentiate into a lymphoid tissue organizer (LTO) phenotype.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL1261

Series:

GSE19139

5 Samples

Download data: CEL

Analysis of cultured aortic smooth muscle cells (SMCs) stimulated with a combination of tumor necrosis factor (TNF) and α-LTβR monoclonal antibody for up to 24 hours. SMCs stimulated by TNF/α-LTβR mAb, but not with either agent alone, differentiate into lymphoid tissue organizer (LTO)-like cells

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 agent, 3 time sets

Platform:

GPL1261

Series:

GSE15062

21 Samples

Download data: CEL

Temporal analysis of umbilical vein endothelial cells (HUVEC) treated with leukotriene D4 (LTD4) and/or thrombin. LTD4 activates the cysteinyl leukotriene receptor cysLT2-R. Thrombin activates the protease-activated receptor PAR-1. cysLT2-R and PAR-1 may cooperate to augment vascular injury.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 4 agent, 3 time sets

Platform:

GPL96

Series:

GSE3589

21 Samples

Download data

(Submitter supplied) Tertiary lymphoid organs (TLOs) emerge in response to nonresolving inflammation but their roles in adaptive immunity remain unknown. Here, we explored artery TLOs (ATLOs) to delineate atherosclerosis T cell responses in apoe-/- mice during aging. Though the T cell repertoire showed systemic age-associated contractions in size and modifications in subtype composition and activation, wt and apoe-/- mice were equally affected. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL8321 GPL1261

65 Samples

Download data: CEL, EXP

(Submitter supplied) Background: Lymphotoxin signaling via the lymphotoxin-β receptor (LTβR) has been implicated in several biological processes, ranging from development of secondary lymphoid organs, maintenance of splenic tissue, host defense against pathogens, autoimmunity, and lipid homeostasis. The major transcription factor that is activated by LTβR crosslinking is NF-κB. Two signaling pathways have been described that result in the activation of classical p50-RelA and alternative p52-RelB NF-κB heterodimers. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL5979

18 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL18387

12 Samples

Download data: IDAT

(Submitter supplied) To investigate the regulatory mechanisms and targets of the activation of NF-kB and inflammatory pathways, we treated HPV(-) head and neck cancer line UM-SCC 46 cells with TNFα and LTβ at different time points, and compared the gene expression by microarray. TNFα uniquely induced 172 genes, LTβ specifically induced 202 genes, while 155 genes were induced by both ligands. Total RNA samples were isolated from UM-SCC 46 cells after TNFα or LTβ treatment at different time points, and the gene expression were compared with untreated cell controls.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL18387

6 Samples

Download data: IDAT, XLSX

(Submitter supplied) To investigate the regulatory mechanisms and targets of the activation of NF-kappaB and inflammatory pathways, we treated HPV(-) head and neck cancer line UM-SCC 46 cells with TNFα and LTβ at different time points, and compared the gene expression by microarray. TNFα uniquely induced 172 genes, LTβ specifically induced 202 genes, while 155 genes were induced by both ligands. Total RNA samples were isolated from UM-SCC 46 cells after TNFα or LTβ treatment at different time points, and the gene expression were compared with untreated cell controls.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL18387

6 Samples

Download data: IDAT, XLSX

(Submitter supplied) Developmental signals are known to modulate inflammation. How ever, the mechanistic insight that links developmental and inflammatory signaling remains elusive. In the current study, we identifya critical role of NF-kB system in mediating stimulus specific crosstalk that allows developmental LTbR signals to sustain inflammatory TLR4 induced RelA/NF-kB response and gene expression. LTbR activated non-canonical signaling targets canonical TLR4 induced, nfkb2 encoded p100 not only to deplete inhibitory IkBd/(p100)2, but also to supplement RelA:p52/NF-kB dimers. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5655

Platform:

GPL6885

4 Samples

Download data: TXT

Analysis of MEFs treated for 24hrs with ligands lipopolysaccharide (LPS), lymphotoxin-β receptor agonist antibody (LTβR), and LPS+ LTβR. Results provide insight into molecular mechanisms underlying a potentially synergistic effect of LTβR signaling on LPS-TLR4 signaling.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 protocol sets

Platform:

GPL6885

Series:

GSE62301

4 Samples

Download data

(Submitter supplied) We used manual macrodissection or laser capture microdissection (LCM) to isolate tissue sections of the hippocampus area of Ras-GRF1 wild type and knockout mice brains, and analyzed their transcriptional patterns using commercial oligonucleotide microarrays. Comparison between the transcriptomes of macrodissected and microdissected samples showed that the LCM samples allowed detection of significantly higher numbers of differentially expressed genes, with higher statistical rates of significance. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2816

Platform:

GPL339

6 Samples

Download data: CEL, CHP

Analysis of laser capture microdissected hippocampi of mutants lacking Ras-GRF1. Ras-GRF-1 is a guanine nucleotide exchange factor predominantly expressed in the central nervous system of mammals. Results provide insight into role of Ras-GRF1 in neural processes.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL339

Series:

GSE8425

6 Samples

Download data: CEL, CHP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2872

4 Samples

Download data: TXT

(Submitter supplied) This study compared gene expression in smooth muscle cells (SMCs) in atherosclerosis-prone and atherosclerosis-resistant aorta segments in 4 months old apolipoprotein E-deficient (apoE-/-) mice before plaque development. In a parallel experiment, both regions were compared in young C57Bl/6 mice. Aortas of 3 male and 3 female ApoE-/- mice were isolated, perfused with triton X-100 to remove endothelial cells and divided in an atherosclerosis-prone region (AA: ascending aorta, aortic arch and proximal 2 mm of thoracic aorta) and a resistant region (TA: central thoracic aorta, i.e. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2872

2 Samples

Download data: TXT

(Submitter supplied) This study compared gene expression in smooth muscle cells (SMCs) in atherosclerosis-prone and atherosclerosis-resistant regions of the aorta of C57Bl/6 mice. In a parallel experiment, both regions were compared in young, plaque-free apolipoprotein E-deficient (apoE-/-) mice. Aortas of 3 male and 3 female C57Bl6 mice were isolated, perfused with triton X-100 to remove endothelial cells and divided in an atherosclerosis-prone region (AA: ascending aorta, aortic arch and proximal 2 mm of thoracic aorta) and an atherosclerosis-resistant region (TA: central thoracic aorta,i.e. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2872

2 Samples

Download data: TXT