GEO DataSets

(Submitter supplied) Microarray-based studies of skeletal muscle from patients with type 2 diabetes and high-risk individuals have demonstrated that insulin resistance and reduced mitochondrial biogenesis co-exist early in the pathogenesis of type 2 diabetes independent of hyperglycaemia and obesity. It is unknown whether reduced mitochondrial biogenesis or other transcriptional alterations co-exist with impaired insulin-responsiveness in primary human muscle cells from patients with type 2 diabetes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3681

Platform:

GPL8300

20 Samples

Download data: CEL

Analysis of myotube cell lines established from type 2 diabetes (T2D) subjects. Insulin resistance and reduced mitochondrial biogenesis coexist early in T2D pathogenesis independent of hyperglycemia and obesity. Results provide insight into the effect of T2D on developing skeletal muscle cells.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 disease state sets

Platform:

GPL8300

Series:

GSE12643

20 Samples

Download data: CEL

(Submitter supplied) Recently, abnormalities in mitochondrial oxidative phosphorylation (OXPHOS) have been implicated in the pathogenesis of skeletal muscle insulin resistance in type 2 diabetes. In the present study, we hypothesized that decreased expression of OXPHOS genes could be of similar importance for insulin resistance in the polycystic ovary syndrome (PCOS). Using the HG-U133 Plus 2.0 expression array from Affymetrix, we analyzed gene expression in skeletal muscle from obese women with PCOS (n=16) and age- and body mass index-matched control women (n=13) metabolically characterized by euglycemic-hyperinsulinemic clamp and indirect calorimetry. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3104

Platform:

GPL570

29 Samples

Download data: CEL

Analysis of vastus lateralis muscles from women with insulin-resistant polycystic ovary syndrome (PCOS). Insulin resistance in skeletal muscles is a risk factor for the development of type 2 diabetes in women with PCOS. Results provide insight into the pathogenesis of insulin resistance in PCOS.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 disease state sets

Platform:

GPL570

Series:

GSE6798

29 Samples

Download data: CEL

(Submitter supplied) Type 2 diabetes mellitus (DM) is characterized by insulin resistance and pancreatic beta-cell dysfunction. In high-risk subjects, the earliest detectable abnormality is insulin resistance in skeletal muscle. Impaired insulin-mediated signaling, gene expression, and glycogen synthesis, and accumulation of intramyocellular triglycerides have all been linked with insulin resistance, but no specific defect responsible for insulin resistance and DM has been identified in humans. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL80

20 Samples

Download data: CEL

(Submitter supplied) We used microarrays to assess gene expression profiling of 6 patients with a mutation (Arg1174Gln) in the tyrosine kinase domain of the insulin receptor gene (INSR) and 10 matched healthy controls

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4897

Platform:

GPL571

16 Samples

Download data: CEL

Analysis of muscle from patients with a mutation (Arg1174Gln) in the tyrosine kinase domain of the insulin receptor gene (INSR). This mutation is associated with inherited insulin resistance. Results provide insight into molecular mechanisms underlying insulin resistance in skeletal muscle.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL571

Series:

GSE36297

16 Samples

Download data: CEL

(Submitter supplied) The aim of this study was therefore to investigate molecular mechanisms associated with insulin sensitivity in skeletal muscle by relating global skeletal muscle gene expression with a surrogate measure of insulin sensitivity, i.e. homeostatic model assessment of insulin resistance (HOMA-IR). To identify genes with skeletal muscle expression related to insulin sensitivity, we obtained muscle biopsies from 38 non-diabetic participants in study A. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL7144 GPL4133

47 Samples

Download data: CEL, TXT

(Submitter supplied) We studied 9 healthy young non-diabetic men without any family history of diabetes. The mean age and body mass index (BMI) were 25.33 ± 0.33 years and 24.57 ± 0.62 kg/m2, respectively, and the mean 1/ homeostatic model assessment of insulin resistance (HOMA-IR) was 1.17 ± 0.12. We included baseline gene expression profile data (i.e. only before bed rest)

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

9 Samples

Download data: TXT

(Submitter supplied) Rationale: Physical inactivity is a risk factor for insulin resistance. We examined the effect of nine days of bed rest on basal and insulin stimulated expression of genes potentially involved in insulin action by applying hypothesis-generating microarray in parallel with candidate gene real-time PCR approaches in 20 healthy, young men. Furthermore, we investigated whether bed rest affected DNA methylation in the promoter region of the peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PPARGC1A) gene. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

60 Samples

Download data: TXT

(Submitter supplied) We identified the target genes of FTO ("fat mass and obesity associated") in primary cultures of human skeletal muscle cells using adenoviral vectors expressing FTO or GFP and oligonucleotide microarrays.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1456

4 Samples

Download data: GPR

(Submitter supplied) 6 lean humans were submitted to a 3 hours hyperinsulinemic-euglycemic clamp. Skeletal muscle biopsies were taken before and after the clamp. Set of arrays that are part of repeated experiments

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6991

6 Samples

Download data

(Submitter supplied) To identify mediators of obesity-linked reductions in PGC-1, we tested the effects of cellular nutrients in C2C12 myotubes. While overnight exposure to high insulin, glucose, glucosamine, or amino acids had no effect, saturated fatty acids (FA) potently reduced PGC-1a and b mRNA expression. Keywords: Nutrient Effect

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2648

Platform:

GPL8321

6 Samples

Download data: CEL

Analysis of myoblasts treated with the saturated fatty acid palmitate. Muscle expression of PPAR coactivator 1 (PGC-1) is reduced in models of obesity. Palmitate decreases the expression of PGC-1. Results provide insight into the molecular basis of the link between overnutrition, obesity, and PGC-1.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL8321

Series:

GSE6766

6 Samples

Download data: CEL

(Submitter supplied) Insulin resistance is a common metabolic abnormality in women with PCOS and leads to an elevated risk of type 2 diabetes. Studies have shown that thiazolidinediones (TZD) improve metabolic disturbances in PCOS patients. We hypothesized that the effect of TZD in PCOS is in part mediated by changes in the transcriptional profile of muscle favoring insulin sensitivity. Using Affymetrix microarrays, we examined the effect of pioglitazone (30 mg/day for 16 weeks) on gene expression in skeletal muscle of 10 obese women with PCOS metabolically characterized by a euglycemic-hyperinsulinemic clamp. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS4132 GDS4133

Platform:

GPL570

43 Samples

Download data: CEL

Analysis of skeletal muscle from obese women with polycystic ovary syndrome (PCOS) and obese, healthy women. Results provide insight into whether pioglitazone ameliorates preexisting abnormalities in PCOS patients.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 disease state sets

Platform:

GPL570

Series:

GSE8157

23 Samples

Download data: CEL

Analysis of skeletal muscle from obese women with polycystic ovary syndrome (PCOS) before and after thiazolidinedione (TZD) pioglitazone treatment. TZDs improve metabolic disturbances in PCOS patients. Results provide insight into the molecular mechanisms underlying the effect of TZD in PCOS.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL570

Series:

GSE8157

20 Samples

Download data: CEL

(Submitter supplied) Transcriptional microarray analysis was conducted on gastrocnemius muscle of control and PGC-1β(i)skm-/- mice one week after the last tamoxifen administration using the Affymetrix Mouse Gene 1.0 ST.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

2 Samples

Download data: CEL, CHP

(Submitter supplied) Skeletal muscle is one of the primary tissues involved in the development of type 2 diabetes (T2D). Obesity is tightly associated with T2D, making it challenging to isolate specific effects attributed to the disease alone. By using an in vitro myocyte model system we were able to isolate the inherent properties retained in myocytes originating from donor muscle precursor cells, without being confounded by varying extracellular factors present in the in vivo environment of the donor. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL11154 GPL16791

90 Samples

Download data: TXT

(Submitter supplied) Skeletal myocytes are metabolically active and susceptible to insulin resistance, thus implicated in type 2 diabetes (T2D). This complex disease involves systemic metabolic changes and their elucidation at the systems level requires genome-wide data and biological networks. Genome-scale metabolic models (GEMs) provide a network-context to integrate high-throughput data. We generated myocyte-specific RNA-seq data and investigated their correlation with proteome data. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL16791 GPL11154

6 Samples

Download data: TXT