GEO DataSets

(Submitter supplied) Subcutaneous abdominal adipose tissue was obtained from female subjects undergoing plastic surgery in agreement with French laws on biomedical research. Stromal cells prepared from WAT were cultured for 13 days in a chemically defined medium. At day 13, 60–80% of cells were differentiated into lipid droplet-containing adipocytes. The cells were infected at a m.o.i. of 200 for 6 h. The day after infection, cells were treated with the following drugs at 1 µM unless otherwise indicated: rosiglitazone (BRL49653, Smith Kline and French, Harlow, UK) and 9-cis-RA (Sigma). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL3941 GPL3942

16 Samples

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(Submitter supplied) Glycerol kinase deficiency (GKD) is an X-linked inborn error of metabolism with metabolic and neurologic crises. Liver shows the highest level of glycerol kinase (GK) activity in humans and mice. Absence of genotype-phenotype correlations in patients with GKD indicate the involvement of modifier genes, including other network partners. To understand the molecular pathogenesis of GKD, we performed microarray analysis on liver mRNA from neonatal glycerol kinase (Gyk) knockout (KO) and wild type (WT) mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1555

Platform:

GPL1261

8 Samples

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Expression profiling of livers of 4 male glycerol kinase knockout mutants. Glycerol kinase deficiency (GKD) is an X-linked inborn error of metabolism with metabolic and neurologic crises. Results provide insight into the pathogenesis of GKD.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE3843

8 Samples

Download data

(Submitter supplied) The nuclear receptor PPARalpha is recognized as the primary target of the fibrate class of hypolipidemic drugs and mediates lipid lowering in part by activating a transcriptional cascade that induces genes involved in the catabolism of lipids. We report here the characterization of three novel PPARalpha agonists with therapeutic potential for treating dyslipidemia. These structurally related compounds display potent and selective binding to human PPARalpha and support robust recruitment of coactivator peptides in vitro. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

30 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4044

28 Samples

Download data: TXT

(Submitter supplied) Fenofibrate is a synthetic ligand for the nuclear receptor peroxisome proliferator-activated receptor (PPAR) alpha, but there are reports that fenofibrate affects endothelial cells in PPARa-independent manner. In order to identify PPARa-dependently and PPARa-independently regulated transcripts we generated microarray data from human endothelial cells treated with fenofibrate with and without siRNA-mediated knock-down of PPARa.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4044

19 Samples

Download data: TXT

(Submitter supplied) Fenofibrate is a synthetic ligand for the nuclear receptor peroxisome proliferator-activated receptor (PPAR) alpha, but there are reports that fenofibrate affects endothelial cells in PPARa-independent manner. In order to identify PPARa-dependently and PPARa-independently regulated transcripts we generated microarray data from human endothelial cells treated with fenofibrate with and without siRNA-mediated knock-down of PPARa.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4044

9 Samples

Download data: TXT

(Submitter supplied) A functional interaction between peroxisome proliferator-activated receptor alpha (PPARalpha) and components of the circadian clock has been suggested; however, it remains to be clarified whether those transcriptional factors interact with each other to regulate the expression of their target genes. In this study, we used a ligand of PPARalpha, bezafibrate, to search the PPARalpha-regulated genes that express in a CLOCK-dependent circadian manner. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL

(Submitter supplied) Objective : This work aimed at characterizing PPARα involvement in metabolism regulation and at comparing PPARα and PPARγ roles in human white adipocytes. Research Design and Methods : Profiling of gene expression alterations was assessed with microarrays and RT-qPCR. Primary cultures of human adipocytes were treated with PPARα agonist GW7647 or PPARγ agonist Rosiglitazone. Western blot were used to determine protein level modifications. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1708

8 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL7440 GPL1261

59 Samples

Download data: CEL

(Submitter supplied) Fatty acids comprise the primary energy source for the heart and are mainly taken up via hydrolysis of circulating triglyceride-rich lipoproteins. While most of the fatty acids entering the cardiomyocyte are oxidized, a small portion is involved in altering gene transcription to modulate cardiometabolic functions. So far, no in vivo model has been developed enabling study of the transcriptional effects of specific fatty acids in the intact heart. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7440

4 Samples

Download data: CEL

(Submitter supplied) Fatty acids comprise the primary energy source for the heart and are mainly taken up via hydrolysis of circulating triglyceride-rich lipoproteins. While most of the fatty acids entering the cardiomyocyte are oxidized, a small portion is involved in altering gene transcription to modulate cardiometabolic functions. So far, no in vivo model has been developed enabling study of the transcriptional effects of specific fatty acids in the intact heart. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

55 Samples

Download data: CEL

(Submitter supplied) Analysis of white adipose tissue of PPARb/d knockout mice. Data may point towards putative target genes of PPARb/d and thus the function of PPARb/d in white adipose tissue. Datasets were used to identify glycogen synthase 2 as novel PPAR target. Keywords: gene expression array-based, count

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL339

2 Samples

Download data: CEL

(Submitter supplied) The peroxisome proliferator-activated receptor alpha (PPARα) is a fatty acid-activated transcription factor that governs a variety of biological processes. Little is known about the role of PPARα in the small intestine. Since this organ is frequently exposed to high levels of PPARα ligands via the diet, we set out to characterize the function of PPARα in small intestine using functional genomics experiments and bioinformatics tools. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2886

Platform:

GPL339

12 Samples

Download data: CEL

Analysis of small intestine of wild type and peroxisome proliferator-activated receptor alpha (PPARalpha) null animals after treatment with WY14643, an agonist of PPARalpha. PPARalpha is a fatty acid-activated transcription factor. Results provide insight into role of PPARalpha in small intestine.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 2 genotype/variation sets

Platform:

GPL339

Series:

GSE5475

12 Samples

Download data: CEL

(Submitter supplied) Mutations in the gene encoding lipin 1 cause hepatic steatosis in fld mice, a genetic model of lipodystrophy. Lipin 1 appears to be highly involved in the control of fatty acid metabolism. Lipin 1 is most often located in the nucleus, but other studies suggest that lipin also has effects in the cytoplasm. However, the molecular function of lipin 1 is unclear. To evaluate the effects of activation of the lipin 1 system in liver, lipin 1beta was overexpressed in mouse liver using an adenoviral vector. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2291

Platform:

GPL339

4 Samples

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Analysis of livers of transgenics overexpressing lipin 1-beta, an alternative form of lipin 1 containing a 33 amino acid insertion. Mutations in the gene encoding lipin 1 cause hepatic steatosis in fld animals, a genetic model of lipodystrophy. Results provide insight into the function of lipin 1.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL339

Series:

GSE5538

4 Samples

Download data

(Submitter supplied) This study aimed at determining the transcriptional changes associated with the white-to-brown conversion of human mesenchymal adipose-derived stem cells firstly differentiated into white adipocytes (in the presence of rosiglitazone from day 2 to day 9). White differentiation was completed within 14 days, and PPARg (rosiglitazone) or PPARa (GW7647) agonists were added to the medium for 4 additional days to induce the brown phenotype. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13497

12 Samples

Download data: GPR

(Submitter supplied) Kupffer cells have been implicated in the pathogenesis of various liver diseases. However, their involvement in metabolic disorders of the liver, including fatty liver disease, remains unclear. The present study sought to determine the impact of Kupffer cells on hepatic triglyceride storage and to explore the possible mechanisms involved. To that end, C57Bl/6 mice rendered obese and steatotic by chronic high-fat feeding were treated for 1 week with clodronate liposomes, which cause depletion of Kupffer cells. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4166

Platform:

GPL1261

4 Samples

Download data: CEL

Analysis of liver from C57BL/6 males fed high fat diets (HFD) for 20 wks to induce obesity and hepatic steatosis, and injected with clodronate during the last week of HFD to deplete Kupffer cells (KCs) in liver. Results provide insight into role of KCs in regulation of hepatic triglyceride storage.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 disease state, 2 growth protocol, 2 protocol sets

Platform:

GPL1261

Series:

GSE33044

4 Samples

Download data: CEL