GEO DataSets

(Submitter supplied) The objective of the present study was to use our own fabricated bovine embryo cDNA microarray to profile genome-wide expression patterns of genes during the implantation period. Duplicate readings were obtained by hybridization of a Cy3/Cy5 probe mixture for each sample. Data normalization was performed the local background intensity of each array spot was smoothed by the local weight regression (lowess) smoother and subtracted from spot intensity data. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Dataset:

GDS1003

Platform:

GPL1221

11 Samples

Download data: TIFF

Expression profiling of embryos at various days of gestation up to 21 days and fetuses and fetal membranes at 27 and 28 days of gestation. Results provide insight into the regulation of embryonic, fetal, and fetal membrane differentiation.

Organism:

Bos taurus

Type:

Expression profiling by array, count, 7 development stage, 3 tissue sets

Platform:

GPL1221

Series:

GSE1414

11 Samples

Download data: TIFF

(Submitter supplied) Blastocyst formation is a primordial event of pre-implantation development since it is required for pregnancy establishment and progression. The blastocyst plays a pivotal role because it is the stage at which the embryo is transferred and starts coordinated cross-talks with the mother. It is also the terminal step of pre-implantation developmentbefore transfer; it reflects all stresses the embryo may have faced during the process of in-vitro treatment. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11247

9 Samples

Download data: TXT

(Submitter supplied) Gene expression patterns of bovine placenta from initial to late stage of pregnancy were investigated by using the our custom designed utero-placental cDNA microarray. Keywords: Time course

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL1221

18 Samples

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(Submitter supplied) BPL_array_NIAS platform of a spotted cDNA microarray has been established using a bovine utero-placenta cDNA library. The array has been fabricated collaboratively by Laboratory of Reproductive Biology and Technology at National Institute of Agrobiological Sciences, Tsukuba City, Japan and Department of Paediatric Pharmacology at National Institute of Child Health and Development, Tokyo, Japan. Keywords = bovine utero-placenta Keywords = cDNA array Keywords = gene expression

Organism:

Bos taurus

1 DataSet

2 Series

29 Samples

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(Submitter supplied) Axis specification in mouse is determined by a sequence of reciprocal interactions between embryonic and extra-embryonic tissues so that a few extra-embryonic genes appear as ‘patterning’ the embryo. Considering these interactions as essential, but lacking in most mammals the genetically driven approaches used in mouse and the corresponding patterning mutants, we examined whether a molecular signature originating from extra-embryonic tissues could relate to the developmental stage of the embryo proper and predict it. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL7417

20 Samples

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(Submitter supplied) During mammalian pre-implantation embryonic development dramatic and orchestrated changes occur in gene transcription. The identification of the complete changes has not been possible until the development of the Next Generation Sequencing Technology. Here we report the first transcriptome dynamics of single matured bovine oocytes and all stages of pre-implantation embryos developed in vivo. Surprisingly, nearly half of the bovine genome, 11,488 to 12,729 genes involved in more than 100 pathways, is expressed in oocytes and early embryos. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18907

16 Samples

Download data: TXT

(Submitter supplied) Temporal changes in the embryo transcriptome between the blastocyst stage (Day 7) and initiation of elongation (Day 13) differ between in vivo- and in vitro-derived embryos and are reflective of subsequent developmental fate. The aim of this study was to examine the temporal changes in transcriptional profile as the embryo develops from a spherical blastocyst on Day 7 to an ovoid conceptus at the initiation of elongation on Day 13 and to highlight differences in these temporal gene expression dynamics between in vivo- and in vitro-derived blastocysts which may be associated with embryonic survival/mortality using the bovine Affymetrix microarray.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

12 Samples

Download data: CEL

(Submitter supplied) This cDNA clone set was prepared by researchers at the National Institute on Aging. The clone set has been distributed widely and is currently in use by a number of other microarray programmes. The set has the following characteristics: ~ 15,000 'unique' cDNA clones were rearrayed from 52,374 ESTs from pre- and periimplantation embryos, E12.5 female gonad/mesonephros, and newborn ovary. Up to 50% are derived from novel genes. more...

Organism:

Mus musculus

2 Series

8 Samples

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(Submitter supplied) HGMP printed NIA 15K arrays, printed on 2 slides, 17280 spots per slide, including control spots, empties and landmarks. 2 duplicate spots per slide for each clone. Earlier versions of these slides have already been described by platform entries GPL5457 and GPL5458. However, owing to analysis of a later array print run by a different image analysis programme in this series of experiments, the data files present in a different clone order and it is impossible to sort them to generate the same order. more...

Organism:

Mus musculus

1 Series

18 Samples

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(Submitter supplied) Second slide of 2. HGMP printed NIA 15K set, over 2 slides, 17280 spots per slide, including empties, control spots, landmark spots, and duplicate experimental spots. 12 rows and 4 columns of grids, each grid contains 18 rows and 20 columns of spots (format for both slides 1-20 and 21-40). Protocol: cDNA arrays (now discontinued), spotted PCR product. Bacterial source material diluted 1/10 prior to PCR, primers: Ko forward primer (amidated): GTG TGG AAT TGT GAG CGG ATA ACA A (25mer) with 5' NH2(C6) modification. more...

Organism:

Mus musculus

1 Series

10 Samples

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(Submitter supplied) HGMP printed NIA 15K arrays, printed on 2 slides, 17280 spots per slide, including control spots, empties and landmarks. 2 duplicate spots per slide for each clone. This is the platform submission for Slide 1 Protocol: cDNA arrays (now discontinued), spotted PCR product. Bacterial source material diluted 1/10 prior to PCR, primers: Ko forward primer (amidated): GTG TGG AAT TGT GAG CGG ATA ACA A (25mer) with 5' NH2(C6) modification. more...

Organism:

Mus musculus

1 Series

10 Samples

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(Submitter supplied) cDNA array created by amplification of the National Institute of Aging 15K clone set. All clones were PCR amplified using M13 forward and reverse primers, cleaned with millipore filter plates and printed in 3xSSC and 1.5M betaine with a Genomic Solutions OmniGrid 100 robot. Arrays were printed on Corning GAPSII amino coated slides. 32 Spalax sequences were added to the array.

Organism:

Mus musculus; Nannospalax ehrenbergi

1 Series

12 Samples

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(Submitter supplied) Custom array including the NIA Mouse 15K Cloneset plus addition unidentified clones Keywords = Mouse NIA 15K Arizona

Organism:

Mus musculus

2 DataSets

2 Series

24 Samples

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(Submitter supplied) Pre-implantation embryos derived by in vitro fertilization differ in their developmental potential from embryos obtained in vivo. In order to characterize changes in gene expression profiles caused by in vitro culture environment, we employed microarray constructed from bovine embryo-specific cDNAs (BlueChip v., Université Laval, Québec) to compare in vitro cultured and in vivo derived bovine embryos at 4-cell and 8-cell stage.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11061

6 Samples

Download data: MEV

(Submitter supplied) In addition to nourishing the embryo, extra-embryonic tissues (EETs) contribute to early embryonic patterning, primitive hematopoiesis, and fetal health. These tissues are of major importance for human medicine, as well as for efforts to improve livestock efficiency, but they remain incompletely understood. In bovines, EETs are accessible easily, in large amounts, and prior to implantation. We took advantage of this system to describe, in vitro and in vivo, the cell types present in bovine EETs at Day 18 of development. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL7417

14 Samples

Download data: TXT

(Submitter supplied) In animals, the maternal-to-embryonic transition (MET) is an important step occurring in the first days of early development. This important transition involves the degradation of maternal transcripts that have been stocked during oogenesis and used until this transition. Moreover, some precise and specific control mechanisms must govern the adequate synchronization of the MET events to promote embryonic genome activation. more...

Organism:

Saguinus imperator; Pan troglodytes; Bos taurus; Ovis aries; Rattus norvegicus; Ateles sp.; Macaca mulatta; Pan paniscus; Monodelphis domestica; Symphalangus syndactylus; Gorilla gorilla; Pongo abelii; Homo sapiens; Sus scrofa; Cricetulus griseus; Mus musculus; Lemur catta; Lagothrix; Macaca nemestrina; Canis lupus familiaris; Rhinopithecus bieti

Type:

Expression profiling by array

Platform:

GPL15194

1 Sample

Download data: TXT

(Submitter supplied) Studies on embryo cryopreservation efficiency had been focused mainly on technical and embryo factors. While structural damages can be easily evaluated, physiological damages only can be estimated by analyzing their in vitro and in vivo development to later stages. In order to determine how cryopreservation process affect embryo pre-implantory development, a transcriptional microarray study has been performed comparing gene expression of 6 days old rabbit embryos, previously vitrified or frozen and transferred into recipients rabbit females, to their in vivo counterparts. more...

Organism:

Oryctolagus cuniculus

Type:

Expression profiling by array

Platform:

GPL7083

6 Samples

Download data: TXT, XLS

(Submitter supplied) This study describes the generation and analysis of the transcriptional profile of bovine inner cell mass (ICM) and trophectoderm (TE), obtained from in vivo developed embryos by using a bovine-embryo specific array (EmbryoGENE) containing 37,238 probes.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

8 Samples

Download data: TXT

(Submitter supplied) Using microarrays, genome-wide RNA expression was profiled and compared for in vitro fertilization (IVF) - derived blastocysts and embryos undergoing degenerative development up to the same time point. Surprisingly similar transcriptomic profiles were found in degenerative embryos and blastocysts. Nonetheless, we identified 67 transcripts that significantly differed between these two groups of embryos at a 15% false discovery rate, including 33 transcripts showing at least a two-fold difference. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

6 Samples

Download data: CEL