GEO DataSets

(Submitter supplied) 10,185 high-quality sequences were obtained from 10 tissue(or stage of development)-specific libraries and were submitted to GenBank (accession numbers: FF281814-FF291996) The final array design contained 5,087 gene specific Solea senegalensis probes, as well as positive and negative control probes (maize expansin, ZmMYB42, and xyloglucan endo-transglycosylase), a total of 700 per array. These were used to assess detection limits and range, to verify spatial homogeneity, and to determine experimental within-array variation. more...

Organism:

Solea senegalensis

10 Series

21 Samples

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(Submitter supplied) A microarray platform for Solea solea was developed to study gene expression profiles of common sole developmental stages.

Organism:

Solea solea

Type:

Expression profiling by array

Platform:

GPL16124

31 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Solea senegalensis

Type:

Expression profiling by array

Platform:

GPL8931

12 Samples

Download data: TXT

(Submitter supplied) Transcriptionnal profile changes in ovary during the reproductive cycle of Solea senegalensis: from previtellogenic stage to vitellogenic stage obtained at different times during the annual reproductive cycle. An oligo microarray based on ESTs sequenced from S. senegalensis previously designed (Cerdà et al., 2008, BMC genomics) was used.

Organism:

Solea senegalensis

Type:

Expression profiling by array

Platform:

GPL8931

3 Samples

Download data: TXT

(Submitter supplied) Transcriptionnal profile changes in ovary during the reproductive cycle of Solea senegalensis: from mature stage to atresia stage obtained at different times during the annual reproductive cycle. An oligo microarray based on ESTs sequenced from S. senegalensis previously designed (Cerdà et al., 2008, BMC genomics) was used.

Organism:

Solea senegalensis

Type:

Expression profiling by array

Platform:

GPL8931

3 Samples

Download data: TXT

(Submitter supplied) Transcriptional profile changes in ovary during the reproductive cycle of Solea senegalensis: from vitellogenic stage to atresia stage obtained at different times during the annual reproductive cycle. An oligo microarray based on ESTs sequenced from S. senegalensis previously designed (Cerdà et al., 2008, BMC genomics) was used.

Organism:

Solea senegalensis

Type:

Expression profiling by array

Platform:

GPL8931

3 Samples

Download data: TXT

(Submitter supplied) Transcriptionnal profile changes in ovary during the reproductive cycle of Solea senegalensis: from vitellogenic stage to mature stage obtained at different times during the annual reproductive cycle. An oligo microarray based on ESTs sequenced from S. senegalensis previously designed (Cerdà et al., 2008, BMC genomics) was used.

Organism:

Solea senegalensis

Type:

Expression profiling by array

Platform:

GPL8931

3 Samples

Download data: TXT

(Submitter supplied) A microarray platform for Solea solea was employed to study gene expression profiles of common sole developmental stages.

Organism:

Solea solea

Type:

Expression profiling by array

Platform:

GPL16124

39 Samples

Download data: TXT

(Submitter supplied) From a set of 15 standard, normalized or full-length cDNA libraries, we generated 139 007 3'- or 5'-end sequenced ESTs, representing more than one-third of the c. 385 000 publicly available Populus ESTs. Clustering and assembly of 107 519 3'-end ESTs resulted in 14 451 contigs and 20 560 singletons, altogether representing 35 011 putative unique transcripts, or potentially more than three-quarters of the predicted c. more...

Organism:

Populus trichocarpa x Populus deltoides; Populus

Type:

Expression profiling by array

Platform:

GPL5921

10 Samples

Download data: TIFF

(Submitter supplied) Corning UltraGaps Protocol: The 15,496 clones from 14 poplar cDNA libraries (Ralph et al., 2006) were selected based on a CAP3 assembly (Huang and Madan, 1999; 95% identity and 40 bp overlap) of ca. 37,000 high-quality 3’ ESTs (i.e. longest available EST sequence from each putative unique transcript). Following in silico selection of candidates, clones were robotically rearrayed from daughter glycerol stock 384-well plates into 96-well plates prefilled with 7% glycerol in LB + ampicillin, incubated overnight at 37°C, and checked for uniform optical density. more...

Organism:

Populus

8 Series

158 Samples

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(Submitter supplied) This is a chicken multi-tissue cDNA microarray with 12752 elements. It comprises clones from chicken EST collections generated by BBSRC, University of Delaware and the Fred Hutchinson Cancer Research Center. Sequence information is available from GenBank for all features on the array. Protocol: The clone-set was amplified in 96 well plates and re-arrayed into a 384-well Montage (Millipore) filtration plate to isolate the amplified DNA products. more...

Organism:

Gallus gallus

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(Submitter supplied) Background The application of microarray technology to functional genomic analysis in the chicken has been limited by the lack of arrays containing large numbers of genes. Results We have produced cDNA arrays using chicken EST collections generated by BBSRC, University of Delaware and the Fred Hutchinson Cancer Research Center. From a total of 363,838 chicken ESTs representing 24 different adult or embryonic tissues, a set of 11,447 non-redundant ESTs were selected and added to an existing collection of clones (4,162) from immune tissues and a chicken bursal cell line (DT40). more...

Organism:

Gallus gallus

5 Series

60 Samples

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(Submitter supplied) series of experiments assesing false positive and background of pig brain library (PBL) microarray Keywords = porcine brain, pig brain Keywords: repeat sample

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL336

2 Samples

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(Submitter supplied) Vocal learning and neuronal replacement have been studied extensively in the songbird brain, but until recently, few molecular and genomic tools have been available for this work. Here we describe new molecular/genomic resources for songbird research. We made cDNA libraries from zebra finch (Taeniopygia guttata) brains at different developmental stages. A total of 11000 clones were sequenced from these libraries, representing 5870 unique gene transcripts. more...

Organism:

Taeniopygia guttata; Serinus canaria

Type:

Expression profiling by array

Platform:

GPL4526

24 Samples

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(Submitter supplied) van Hemert, S., B. H. Ebbelaar, M. A. Smits, and J. M. J. Rebel. 2003. Generation of EST and microarray resources for functional genomic studies on chicken intestinal health. Anim. Biotechnol. 14:133-143.

Organism:

Gallus gallus

2 Series

64 Samples

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(Submitter supplied) Identification and annotation of all the genes in the sequenced Drosophila genome is a work in progress. Wild-type testis function requires many genes and is thus of potentially high value for the identification of transcription units. We therefore undertook a survey of the repertoire of genes expressed in the Drosophila testis by computational and microarray analysis. We generated 3141 high-quality testis expressed sequence tags (ESTs). more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL5

8 Samples

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(Submitter supplied) The array was assembled using 1,681 testes ESTs, representing 1,527 Drosophila melanogaster EST sequences and 144 anonymous clones from the bs collection (CLONE_ID). An aliquot of the same DNA preparation used in the sequencing reactions (GB_ACC), diluted 2/50 in TE, provided material for the PCRs. Inserts were PCR amplified (1X PCR buffer [Life Technologies], 1.5 mM MgCl2, 0.2 mM dNTPs, 1 µM T7 22-mer primer, 1 µM T3 20-mer primer, 2 µl of plasmid DNA dilution [approximately 10 ng], 0.035 U/µl recombinant Taq DNA polymerase [Life Technologies], in a volume of 100 µl, cycled 94C 2 min, 30X[94 C 0.5 min, 55 C 0.5 min, 2.5 min 72 C], 10 min 72 C in a PTC-225 DNA Engine Tetrad [MJ Research]), and successful amplification was confirmed by agarose gel electrophoresis (PCR-QC: passed = product present, failed = no product). more...

Organism:

Drosophila melanogaster

1 Series

8 Samples

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(Submitter supplied) Betanodaviruses (VNNV) are the causative agents of the viral nervous necrosis, a disease that affects cultured Senegalese sole (Solea senegalensis). VNNV reassortant strains, combining genomic segments from the RGNNV and SJNNV genotypes, have previously been isolated from wild and farmed several fish species. The wild reassortant wSs160.03 isolated from sole has been proved to be more virulent for sole than the parental genotypes (RGNNV and SJNNV), causing 100% mortality. more...

Organism:

Solea senegalensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23830

18 Samples

Download data: TXT

(Submitter supplied) cDNAs are arrayed in Genetix 384 well plates and stored frozen. Plates are thawed and spun down. Plates are placed in storage hotel of an Omnigrid 100 (Genomic Solutions) Slides are printed in batches of 100 using 1.5M Betaine + 3 x SSC Following printing slides are baked at 80C for 2 hours Slides are washed in 0.2% SDS followed by three water washes and an ethanol wash Slides are dried by spinning in a centriguge at 1000 RPM for 3 minutes Slides are stored in orange Corning boxes 25 per box Storage boxes are purged with argon and sealed under vacuum

Organism:

Taeniopygia guttata

22 Series

692 Samples

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(Submitter supplied) An EST database from immune tissues was used to design the first high density turbot (Scophthalmus maximus) oligo-microarray with the aim of identifying candidate genes for tolerance to pathogens. Specific oligonucleotides (60mers) were successfully designed for 2716 out of 3482 unique sequences of the database. The performance of the microarray and the sources of variation along microarray analysis were examined on spleen pools of controls and Aeromonas salmonicida challenged fish at 3 days post-infection. more...

Organism:

Scophthalmus maximus

Type:

Expression profiling by array

Platform:

GPL8937

32 Samples

Download data: TXT