GEO DataSets

(Submitter supplied) We investigated the effect of antenatal corticosteroid exposure (ACS) on the DNA methylation patterns in the whole blood of female guinea pig offspring. Guinea pig dams were treated with saline or betamethasone on gestational days 50/51. Whole blood was collected from newborn offspring (post-natal day 1) and dried on blood spot cards. Methylation assessment using genomic DNA was performed with reduced representation bisulfite sequencing techniques.

Organism:

Cavia porcellus

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL29163

14 Samples

Download data: TXT

(Submitter supplied) With more than 130 clinical trials and eight approved gene therapy products, AAVs stand as one of the most popular vehicles to deliver therapeutic DNA in vivo. One critical quality attribute analyzed in AAV batches is the presence of residual DNA, as it could pose genotoxic risks or induce immune responses. Surprisingly, the presence of small cell‑derived RNAs, such as micro‑RNAs, has not been previously investigated. more...

Organism:

unclassified Adeno-associated dependoparvovirus A; Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL34639

3 Samples

Download data: TXT

(Submitter supplied) In this study, single-cell sequencing of the BALF of CARDS patients yielded the following results: (1) IL1B+ neutrophils were significantly increased in the BALF of CARDS patients with exacerbated disease, which was associated with poor prognosis of CARDS patients, and this type of neutrophils underwent fatty acid metabolism reprogramming in comparison with other types of neutrophils, which may be related to their high-inflammatory response. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

16 Samples

Download data: MTX, TSV

(Submitter supplied) Unraveling the transcriptional programs that control how cells divide, differentiate, and respond to their environments requires a precise understanding of transcription factors'(TFs) DNA-binding activities. Calling cards (CC) technology uses transposons to capture transient TF binding event at one instant in time and then read them out at a later time. This methodology can also be used to simultaneously measure transcription factor binding and mRNA expression from single cells CC and to record and integrate TF binding events across time in any cell type of interest without the need for purification. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL24676

22 Samples

Download data: TXT

(Submitter supplied) RNA was isolated from plasma of 20 healthy patients and 20 patients with acute pulmonary embolism. Dysregulated microRNA patterns were screened in these samples

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL33921

40 Samples

Download data: TXT

(Submitter supplied) Impact of different anticoagulants on profiles of exRNAs in healthy individuals

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL17853

80 Samples

Download data: TXT

(Submitter supplied) Impact of circadian rhythm on profiles of exRNAs in healthy individuals

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL17853

60 Samples

Download data: TXT

(Submitter supplied) Studying the role of circular RNAs in cancer initiation by profiling the interaction of circRNAs with DNA in human cells. The first step of oncogenesis is the acquisition of a repertoire of genetic mutations to initiate and sustain the malignancy. An important example of this initiation phase in leukaemia is the formation of a potent oncogene by chromosomal translocations between the histone methyltransferase Mixed Lineage Leukaemia (MLL) gene and one of over 100 translocation partner genes. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18573

16 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Other; Genome binding/occupancy profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL16791 GPL24676 GPL18573

75 Samples

Download data: TSV

(Submitter supplied) Calling Cards is a platform technology to record a cumulative history of transient protein-DNA interactions in the genome of genetically targeted cell types. The record of these interactions are recovered by next generation sequencing. Compared to other genomic assays, whose readout provides a snapshot at the time of harvest, Calling Cards enables correlation of historical molecular states to eventual outcomes or phenotypes. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

9 Samples

Download data: TXT

(Submitter supplied) Background/aims: Lung carcinoids are uncommon neuroendocrine tumours. Molecular features of lung carcinoids have been poorly defined. microRNAs (miRNAs) are potent gene expression regulators with important roles in cancer development and progression. However, little is known on the role of miRNAs in the pathogenesis of lung carcinoids. Our goals were to identify commonly deregulated miRNAs in a rare case of lung carcinoid of typical histology with metastasis, as well as map miRNA target genes in pathways potentially associated with disease development and progression. more...

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL18942

6 Samples

Download data: TXT

(Submitter supplied) Identify candidate miRNA biomarkers for AD in CSF.

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR; Non-coding RNA profiling by array

Platform:

GPL32625

99 Samples

Download data: TXT

(Submitter supplied) Exosomes were purified from 250 ul matched plasma and serum samples using ExoQuickTm. The presence of particles consistent in size with exosomes (60-150nm) was confirmed using a Nanosight LM10. miRNA was extracted from exosomes using an miRNeasy Serum/Plasma kit (Qiagen, #217184). miRNA was reversed transcribed using a TaqMan® microRNA Reverse Transcription Kit (Life technologies, #4366596). miRNA profiling was performed with a high throughput TaqMan® OpenArray® Human microRNA panel (Life technologies, #4461104). more...

Organism:

Homo sapiens

Type:

Other

Platform:

GPL18614

40 Samples

Download data: TXT

(Submitter supplied) Context: There is growing evidence on the role of epigenetic regulation of growth, and miRNAs potentially play a role. Objective: To identify changes in circulating miRNAs following GH treatment in subjects with isolated idiopathic GH deficiency (IIGHD) after the first 3 months on treatment, and verify whether these early changes can predict growth response. Methods: The expression profiles of 384 miRNAs were analyzed in serum in 10 prepubertal patients with IIGHD (5 M,5 F) at two time points before starting GH treatment (t-3, t0), and at 3 months on treatment (t+3). more...

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL31199

30 Samples

Download data: XLSX

(Submitter supplied) Ocular Sebaceous Carcinoma (OSC) is a rare malignant tumor for which initial clinical and pathological diagnosis is often incorrect. OSC can mimic Squamous Cell Carcinoma of the Conjunctiva (SCCC). Aim of this study was to find microRNA biomarkers to distinguish OSC and SCCC from normal tissue and from each other. Clinical OSC and SCCC case files and corresponding histopathological slides were collected and reviewed. more...

Organism:

Homo sapiens

Type:

Other

Platform:

GPL32210

44 Samples

Download data: XLSX

(Submitter supplied) Global profiles of 754 human miRNAs from CFBE41o- (F508del/F508del), and IB3 (F508del/W1282X) cell lines, carrying the most common mutations for Cystic Fibrosis, were analysed. 16HBE14o- cells, derived from normal bronchus, were used as calibrator. The relative abundance of each miRNA was calculated and fold-changes≥+2 or ≤-2 (p-value≤0.05) were considered significant. MiRNA target genes and KEGG pathways were identified using miRWalk v.3 and DIANA-mirPath v3.0, respectively and a protein-protein interaction network was performed by STRING 11.0. more...

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL22556

6 Samples

Download data: TXT

(Submitter supplied) Calling cards technology using self-reporting transposons enables the identification of DNA-protein interactions through RNA sequencing . Here, we have drastically reduced the cost and labor requirements of calling card experiments in bulk populations of cells by introducing a DNA barcode into the calling card itself. An additional barcode incorporated during reverse transcription enables simultaneous transcriptome measurement in a facile and affordable protocol. more...

Organism:

Homo sapiens

Type:

Other

Platforms:

GPL16791 GPL15520

46 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Expression profiling by RT-PCR

Platforms:

GPL20277 GPL6244

8 Samples

Download data: CEL

(Submitter supplied) Description of molecular changes of prostate cancer cells with increased in vivo metastatic potential

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL20277

4 Samples

Download data: TXT

(Submitter supplied) MiRNAs play pivotal roles in regulating macrophage functions, including differentiation, polarization, recruitment, and activation of inflammation. in this review, to provide novel insight into macrophage differentiation-specific mitomiR signatures and associated mitochondrial pathways, we performed a global miRNA profiling on extracted mitochondria and total cell content of human macrophages. The THP-1 monocytic cell line was treated with phorbol-12-myristate-13-acetate (PMA) to induce differentiation into macrophages The human monocytic cell line THP-1 cells were differentiated into THP-1 derived macrophages with 40 ng/mL phorbol 12-myristate 13-acetate (PMA),The mitochondrial fraction of the cells were enriched and purified using anti-TOM22 microbeads (Miltenyi Biotec). more...

Organism:

Homo sapiens

Type:

Other

Platform:

GPL27490

4 Samples

Download data: TXT