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Series GSE270691

Query DataSets for GSE270691

Status

Public on Jun 30, 2024

Title

Characterization of Residual MicroRNAs in AAV Vector Batches Produced in HEK293 Mammalian Cells and Sf9 Insect Cells

Platform organism

Homo sapiens

Sample organisms

Homo sapiens; unclassified Adeno-associated dependoparvovirus A

Experiment type

Expression profiling by RT-PCR

Summary

With more than 130 clinical trials and eight approved gene therapy products, AAVs stand as one of the most popular vehicles to deliver therapeutic DNA in vivo. One critical quality attribute analyzed in AAV batches is the presence of residual DNA, as it could pose genotoxic risks or induce immune responses. Surprisingly, the presence of small cell‑derived RNAs, such as micro‑RNAs, has not been previously investigated. In this study, we examined the presence of miRNAs in purified AAV batches produced in mammalian or in insect cells. Our findings revealed that miRNAs were present in all batches, regardless of the production cell line or capsid serotype (2 and 8). Quantitative assays indicated that miRNAs were co‑purified with the rAAV particles in a proportion correlated with their abundance in the production cells. The level of residual miRNAs was reduced via an immunoaffinity chromatography purification process including a tangential flow filtration step or by RNase treatment, suggesting that most miRNA contaminants are likely non encapsidated into AAV particles. In summary, we demonstrate, for the first time, that miRNAs are co-purified with AAV particles. Further investigations are required to determine whether these miRNAs could interfere with the safety or efficacy of AAV-mediated gene therapy.

Overall design

This dataset includes results of a Taqman Low Density Array experiment performed on three samples: HEK293 cells and two replicate AAV-derived samples, The objective is to compare the miRNA profile of the AAV samples to the one of the producer HEK293 cell line.

Contributor(s)