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Series GSE20347 Query DataSets for GSE20347
Status Public on Mar 15, 2011
Title Analysis of gene expression in esophageal squamous cell carcinoma (ESCC)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To characterize gene expression in esophageal squamous cell carcinoma, we examined gene expression in tumor and matched normal adjacent tissue from 17 ESCC patients from a high-risk region of China.
 
Overall design RNA was extracted from 17 micro-dissected tumor and matched normal tissue pairs using the PureLink Micro-to-Midi Total RNA Purification System (Invitrogen, Carlsbad, CA)
Affymetrix HG-U133A 2.0 gene expression arrays were performed according to the manufacturer's directions.
The Robust Multiarray Average (RMA) algorithm implemented in Bioconductor in R was used for background correction and normalization across all samples.
 
Contributor(s) Clifford RJ, Hu N, Lee MP, Taylor PR
Citation(s) 20955586
Submission date Feb 16, 2010
Last update date Dec 06, 2018
Contact name Robert J Clifford
E-mail(s) clifforr@mail.nih.gov
Phone 301-435-1527
Fax 301-480-6641
Organization name NCI
Department CCR
Lab LPG
Street address 2115 E Jefferson
City Rockville
State/province MD
ZIP/Postal code 20850
Country USA
 
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (34)
GSM509787 normal adjacent esophageal tissue from patient E1507
GSM509788 normal adjacent esophageal tissue from patient E1520
GSM509789 normal adjacent esophageal tissue from patient E1521
Relations
BioProject PRJNA125429

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20347_RAW.tar 65.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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