|
| Status |
Public on Mar 15, 2011 |
| Title |
normal adjacent esophageal tissue from patient E1535 |
| Sample type |
RNA |
| |
|
| Source name |
patient E1535
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: normal adjacent esophageal tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from micro-dissected tumor and matched normal tissue using the PureLink Micro-to-Midi Total RNA Purification System (Invitrogen, Carlsbad, CA).
|
| Label |
biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
As per manufacturer (Affymetrix)
|
| Scan protocol |
Arrays were washed and stained in the Genechip Fluidics Station 450 (Affymetrix) following the manufacturer’s instructions. Chips were scanned with the Affymetrix GeneChip Scanner 3000 using GeneChipOperating System 1.4
|
| Description |
n/a
|
| Data processing |
RMA expression values were generated using the R Bioconductor package.
|
| |
|
| Submission date |
Feb 16, 2010 |
| Last update date |
Mar 15, 2011 |
| Contact name |
Robert J Clifford |
| E-mail(s) |
clifforr@mail.nih.gov
|
| Phone |
301-435-1527
|
| Fax |
301-480-6641
|
| Organization name |
NCI
|
| Department |
CCR
|
| Lab |
LPG
|
| Street address |
2115 E Jefferson
|
| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20850 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE20347 |
Analysis of gene expression in esophageal squamous cell carcinoma (ESCC) |
|
