|
Status |
Public on Mar 15, 2011 |
Title |
esophageal squamous cell carcinoma from patient E1535 |
Sample type |
RNA |
|
|
Source name |
patient E1535
|
Organism |
Homo sapiens |
Characteristics |
tissue: esophageal squamous cell carcinoma
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted from micro-dissected tumor and matched normal tissue using the PureLink Micro-to-Midi Total RNA Purification System (Invitrogen, Carlsbad, CA).
|
Label |
biotin
|
Label protocol |
As per manufacturer (Affymetrix)
|
|
|
Hybridization protocol |
As per manufacturer (Affymetrix)
|
Scan protocol |
Arrays were washed and stained in the Genechip Fluidics Station 450 (Affymetrix) following the manufacturer’s instructions. Chips were scanned with the Affymetrix GeneChip Scanner 3000 using GeneChipOperating System 1.4
|
Description |
n/a
|
Data processing |
RMA expression values were generated using the R Bioconductor package.
|
|
|
Submission date |
Feb 16, 2010 |
Last update date |
Mar 15, 2011 |
Contact name |
Robert J Clifford |
E-mail(s) |
clifforr@mail.nih.gov
|
Phone |
301-435-1527
|
Fax |
301-480-6641
|
Organization name |
NCI
|
Department |
CCR
|
Lab |
LPG
|
Street address |
2115 E Jefferson
|
City |
Rockville |
State/province |
MD |
ZIP/Postal code |
20850 |
Country |
USA |
|
|
Platform ID |
GPL571 |
Series (1) |
GSE20347 |
Analysis of gene expression in esophageal squamous cell carcinoma (ESCC) |
|