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Platform GPL3651 Query DataSets for GPL3651
Status Public on Jan 03, 2007
Title Vibrio cholerae Spotted oligonucleotides
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Vibrio cholerae O1 biovar El Tor str. N16961
Manufacturer Tufts New England Medical Center Expression Array Core
Manufacture protocol Each microarray consists of about 4000 DNA oligonucleotides spotted onto UltraGAPS coated slides (Corning) using a Biorobotics MicroGrid II. Oligonucleotides were 70mers designed to correspond to unique sequences within V. cholerae open reading frames (Illumina), plus additional control sequences.
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Submission date Apr 12, 2006
Last update date Jan 03, 2007
Contact name Leyla Slamti
E-mail(s) leyla.slamti@tufts.edu
Organization name Tufts University/HHMI
Department Molecular biology and microbiology
Lab M. K. Waldor
Street address 136 Harrison Ave
City Boston
State/province MA
ZIP/Postal code 02111
Country USA
 
Samples (16) GSM105444, GSM105445, GSM105446, GSM105447, GSM105448, GSM105449 
Series (3)
GSE6097 Transcriptional analysis of a Vibrio choleare rpoH mutant vs wild-type after a heat-shock
GSE54081 Vibrio cholerae N16961 overexpression of CpxR
GSE55037 Vibrio cholerae N16961 cpxA*

Data table header descriptions
ID
Row Position of the spot
Column Position of the spot
ORF Gene name
SEQUENCE Sequence of the oligonucleotide
Length Length of the oligonucleotide
MW Molecular weight of the oligonucleotide
Tm Temperature of melting of the oligonucleotide
SPOT_ID

Data table
ID Row Column ORF SEQUENCE Length MW Tm SPOT_ID
GI_12057212:1257681-1258931 A 1 VC1184 ATTGTGTATGTAGCGCCGCAGTGGCAAAACACGATTCTGCCTTATAAAGTTGAGCCCGCAACCAATCAAG 70 21689 90.9
GI_12057212:2942322-2944493 A 1 VC2758 ATACAGAAACAATTCTGACCTCCAACACCTCGACCATTCCCATTAACCTGCTCGCCAAATCACTCAAGCG 70 21347 90.6
GI_12057212:426732-427382 A 1 VC0400 TTACGCTGTGCGGTAGCGTGCTTATTGTGATGATGCTATTGCTGGGATTGATTGAGCCCGCCTTAAAACA 70 21786 91.4
GI_12057212:c1695314-1695829 A 1 VC1583 CGCTTTCTTCTCTTCTTCGGTTTTGGCTCAAGAAATGACTGTGGTGATGACGGATTTGAGCTCTGGCCAA 70 21688 91.5
GI_12057212:c2525325-2527682 A 1 VC2369 TTGCTACGCTCTTTCATCGACGCATCTCCCGATTTGATCTATTACCGCAACGCCAAAGGTGAGTTTTCTG 70 21506 91
GI_12057212:c851190-851609 A 1 VC0794 TCTCCGTTTTCAACCCTGTTTTTACCACTGGAAGTGACCATGCAATCAACCTCACTGTTCCTCGAAGGGA 70 21484 91.2
GI_12057213:386384-386779 A 1 VCA0420 GGGCTCTGAGGCTGATTTGAATTGCGAGTGTGTGGATCTGATCGTTAGTC 50 15688 86.6
GI_12057213:c313804-313905 A 1 VCA0296 GAAGCTTCGAGGCTTGCTGATTTTCAGCAATTTTGAAACTGTTGAACTTC 50 15519 83
GI_12057213:c787831-788445 A 1 VCA0846 GATTGGGGTGTATGCCTTTATTACCCTGATCGGATTGGCCGTCGTACTGCATCAATCTCCTGTACTGTTT 70 21639 89.1
Phage:1001-1800:POS_1 A 1 GCGGCAAAAAACTACCGTGAAAAGTCGGTGGATGTGGCGGGTTATGATGAACTTGCTGCTTTTGATGATG 70 21942 91.9 CONTROL_POS
Phage:32001-36000:NEG_15 A 1 TTCAGCCAGCACTGTAAGGTCTATCGGATTTAGTGCGCTTTCTACTCGTGATTTCGGTTTGCGATTCAGC 70 21648 89.7 CONTROL_NEG
GI_12057212:1357310-1358650 A 2 VC1282 GGTTGGACGTTTACGCCTGTGTTGCTGGTCGTGGGTTTACTCATCCTTGATCTGCTCATTTACTTACCTT 70 21612 88.8
GI_12057212:1812689-1813705 A 2 VC1679 ATATTGCGCTGCTTGCCGAACATTTTGCTATTCGCATGTGCCGTGAACTACAACTGCCACTCTTTGTTGG 70 21577 91.9
GI_12057212:2227373-2230192 A 2 VC2072 CATGCCGTGGCTAACTGTCGTAATATCGTGATGACCCGTTTGTGTGTGGAGATGTTTTTGGATGCGTTAG 70 21848 90.7
GI_12057212:815337-815528:Inter:NEG_3.1 A 2 GGCTGGAAACAGTTAGGAGCCGTTTTATTGCCAGTGGCATCAGATTGATGGAGAGAAACTGACCATCATA 70 21864 89.5 CONTROL_NEG
GI_12057212:952846-954393 A 2 VC0894 GGGGGTGGAAGTGAAGCATCTGCCTTTCTATAAGCTTGCGACTCAATTTGGTGACCTCGATCAGTCAAAA 70 21782 90.3
GI_12057212:c1936827-1937522 A 2 VC1788 CTATGTCGTGAATATGCCTAATGGCGGTCTTGGTGGTCCTGACGTAAACTTTCCAGATAACAAAAAGCCC 70 21686 88
GI_12057212:c95592-97571 A 2 VC0098 GTTTGTTGATGAGCAGTATAAACAGAGTCAAGAGTACCAACATTTCTGGCAGCGTTTGCGCTCCGGCGAA 70 21791 90.6
GI_12057213:92442-92546 A 2 VCA0081 GCATACTGTTACTATTGATGAGGTCGAGGAGATTTTCCAGCCGTTTGATAGAGAGAATGACAGCTTGTTC 70 21827 85.8
GI_12057213:c485878-486453 A 2 VCA0549 TGATGAAGAGACCGCTAAGTGGGCAGAAATTGGCATGGACGATGATGCGGAAAAACCACGCGATGTAAAT 70 21965 92.7

Total number of rows: 3997

Table truncated, full table size 484 Kbytes.




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