|
| Status |
Public on Sep 01, 2006 |
| Title |
Dicer knockdown clone #2-2 replicate 1 6 days induced |
| Sample type |
RNA |
| |
|
| Source name |
T-REx-293 cells
|
| Organism |
Homo sapiens |
| Characteristics |
embryonic kidney cells
293 cells stably expressing the tet-repressor and the hairpin 2-2 against Dicer, female, 2days induced with 1ug/ml Doycyclin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from cells in a confluent 6-well dish using Absolutely RNA Miniprep Kit (Stratagene)
|
| Label |
biotin
|
| Label protocol |
15 micrograms of biotinylated fragmented cRNA was hybridized to each chip.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
293 cells expressing the tet-repressor protein_ date of analysis_04032005
|
| Data processing |
Affymetrix and Expressionist
|
| |
|
| Submission date |
Feb 15, 2006 |
| Last update date |
Aug 29, 2006 |
| Contact name |
Daniela Schmitter |
| E-mail(s) |
daniela.schmitter@fmi.ch
|
| Phone |
0041616974128
|
| Organization name |
Friedrich Miescher Institut
|
| Department |
Epigenetics
|
| Lab |
Filipowicz
|
| Street address |
Maulbeerstrasse 66
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE4246 |
Analysis of transcripts regulated by Dicer and Argonaute proteins in human HEK-293 cells |
|
