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Series GSE4246 Query DataSets for GSE4246
Status Public on Sep 01, 2006
Title Analysis of transcripts regulated by Dicer and Argonaute proteins in human HEK-293 cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary HEK-293-derived cell lines depleted of Dicer or one of four Argonaute proteins were generated. Microarray analysis of these knock down cell lines and various control cell lines was performed to address how large the proportion of genes is that are regulated by RNA silencing in an individual cell type. Comparison of different arrays revealed commonly (up) regulated genes. The highest overlap was observed in Ago2 and Dicer knockdown cells.
Keywords: different timepoints of induction of stable knock down cell lines compared to various control cell lines
 
Overall design three control cell lines (T-Rex, shRNA, empty vector), two induvidual Argonaute 1 and 4 (Ago1kd, Ago4kd) knock down cell lines, one Argonaute 2 and 3 (Ago2kd, Ago3kd) and two induvidual Dicer knock down cell lines were analyzed as biological replicates
 
Contributor(s) Filkowski J, Tang K, Oakley E, Angeliker H, Sewer A, Zavolan M, Svoboda P, Filipowicz W
Citation(s) 16971455
Submission date Feb 15, 2006
Last update date Mar 25, 2019
Contact name Daniela Schmitter
E-mail(s) daniela.schmitter@fmi.ch
Phone 0041616974128
Organization name Friedrich Miescher Institut
Department Epigenetics
Lab Filipowicz
Street address Maulbeerstrasse 66
City Basel
ZIP/Postal code 4058
Country Switzerland
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (38)
GSM96814 T-REx ctrl replicate 1
GSM96815 T-REx ctrl replicate 2
GSM96816 shRNA ctrl replicate 1
Relations
BioProject PRJNA94909

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