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Sample GSM942218 Query DataSets for GSM942218
Status Public on Jul 31, 2012
Title wt heart, biological rep1
Sample type RNA
Source name Heart from wt 11 days-old mice
Organism Mus musculus
Characteristics strain: C57BL/6
age: 11 days-old
tissue: heart
genotype: wild-type, TK2+/+
Growth protocol Official Swedish regulations for use and care of laboratory animals were followed throughout and the experimental protocol was approved by the local ethical commitee (S104-09, S135-11)
Extracted molecule total RNA
Extraction protocol Muscle tissues were harvested immediatelly after we have sacrificed the mice. Total RNA was extracted using Rneasy kit (QIAGEN), according to manufacturer's instructions.
Label biotin
Label protocol Labelling was done using Ambion WT Expression kit (Life Technologies), according to manufacturer's instructions.
Hybridization protocol Automated with GeneTitan Instrument and Affymetrix GeneChip Command software (AGCC)
Scan protocol Automated with GeneTitan Instrument and Affymetrix GeneChip Command software (AGCC)
Description Gene expression data from heart muscle of 11 days-old wild-type mouse
Data processing Affymetrix Expression Console Software; Summarization: PLIER, Normalization: Global Median
Submission date Jun 04, 2012
Last update date Jul 31, 2012
Contact name João Amorim Paredes
Organization name Karolinska Institute
Department Laboratory Medicine
Lab Anna Karlsson
Street address Karolinska Universitetssjukhuset Huddinge, F68
City Huddinge
State/province Stockholm
ZIP/Postal code 14186
Country Sweden
Platform ID GPL11533
Series (1)
GSE38449 Gene expression analysis in skeletal muscle and heart of 11 days-old TK2 knockout mice

Data table header descriptions
VALUE PLIER normalized signal intensity

Data table
10338001 2061.43
10338002 21.33
10338003 532.41
10338004 64.99
10338005 3.61
10338006 4.28
10338007 4.02
10338008 8.05
10338009 54.29
10338010 2.61
10338011 13.37
10338012 2.92
10338013 1.85
10338014 2.68
10338015 1.69
10338016 19.46
10338017 4376.56
10338018 11.77
10338019 9.19
10338020 27.95

Total number of rows: 35556

Table truncated, full table size 522 Kbytes.

Supplementary file Size Download File type/resource
GSM942218_JP07_WT1_HE_G05.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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