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Sample GSM942215 Query DataSets for GSM942215
Status Public on Jul 31, 2012
Title TK2 KO skel muscle, biological rep1
Sample type RNA
Source name Hind limb skeletal muscle from TK2 KO 11 days-old mice
Organism Mus musculus
Characteristics strain: C57BL/6
age: 11 days-old
tissue: skeletal muscle
genotype: TK2 knockout, TK2-/-
Growth protocol Official Swedish regulations for use and care of laboratory animals were followed throughout and the experimental protocol was approved by the local ethical commitee (S104-09, S135-11)
Extracted molecule total RNA
Extraction protocol Muscle tissues were harvested immediatelly after we have sacrificed the mice. Total RNA was extracted using Rneasy kit (QIAGEN), according to manufacturer's instructions.
Label biotin
Label protocol Labelling was done using Ambion WT Expression kit (Life Technologies), according to manufacturer's instructions.
Hybridization protocol Automated with GeneTitan Instrument and Affymetrix GeneChip Command software (AGCC)
Scan protocol Automated with GeneTitan Instrument and Affymetrix GeneChip Command software (AGCC)
Description Gene expression data from skeletal muscle of 11 days-old TK2 knockout mouse
Data processing Affymetrix Expression Console Software; Summarization: PLIER, Normalization: Global Median
Submission date Jun 04, 2012
Last update date Jul 31, 2012
Contact name João Amorim Paredes
Organization name Karolinska Institute
Department Laboratory Medicine
Lab Anna Karlsson
Street address Karolinska Universitetssjukhuset Huddinge, F68
City Huddinge
State/province Stockholm
ZIP/Postal code 14186
Country Sweden
Platform ID GPL11533
Series (1)
GSE38449 Gene expression analysis in skeletal muscle and heart of 11 days-old TK2 knockout mice

Data table header descriptions
VALUE PLIER normalized signal intensity

Data table
10338001 1663.06
10338002 48.66
10338003 420.57
10338004 63.21
10338005 3.49
10338006 4.44
10338007 5.58
10338008 9.37
10338009 122.5
10338010 2.52
10338011 24.6
10338012 3.26
10338013 2.48
10338014 2.64
10338015 1.84
10338016 70.8
10338017 3647.83
10338018 33.44
10338019 12.08
10338020 68.17

Total number of rows: 35556

Table truncated, full table size 525 Kbytes.

Supplementary file Size Download File type/resource
GSM942215_JP04_TK2_1_SM_D07.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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