|
| Status |
Public on Jul 27, 2012 |
| Title |
Primary medulloblastoma sample_MB-422 |
| Sample type |
RNA |
| |
|
| Source name |
Primary medulloblastoma
|
| Organism |
Homo sapiens |
| Characteristics |
age: 12.0
gender: M
histology: Classic
subgroup: Group 4
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from frozen medulloblastoma tissue using the Trizol method (Invitrogen) as directed by the manufacturer. RNA integrity was assessed using the Agilent 2100 Bioanalyzer prior to processing for the Gene Array. Samples with RIN values ≥7 were included in the study.
|
| Label |
Biotin
|
| Label protocol |
As directed by Affymetrix using 400ng total RNA as starting material.
|
| |
|
| Hybridization protocol |
As directed by Affymetrix.
|
| Scan protocol |
As directed by Affymetrix.
|
| Description |
MB-422
|
| Data processing |
Signal estimates for Gene Level Analysis were determined using Affymetrix Expression Console (1.1) and default paramters. Samples were analyzed in three separate batches according to medulloblastoma subgroup affilliation: SHH, Group 3, and Group 4.
|
| |
|
| Submission date |
Apr 18, 2012 |
| Last update date |
Jul 27, 2012 |
| Contact name |
Paul A. Northcott |
| E-mail(s) |
panorthcott@gmail.com
|
| Organization name |
German Cancer Research Center (DKFZ)
|
| Street address |
Im Neuenheimer Feld 280
|
| City |
HEIDELBERG |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11532 |
| Series (2) |
| GSE37382 |
Subgroup specific somatic copy number aberrations in the medulloblastoma genome [mRNA] |
| GSE37385 |
Subgroup specific somatic copy number aberrations in the medulloblastoma genome |
|
