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Status |
Public on Dec 24, 2013 |
Title |
whole_ctrl_1 |
Sample type |
RNA |
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Source name |
whole kidneys
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Organism |
Rattus norvegicus |
Characteristics |
gender: male strain: Wistar/ST age: 8 weeks old tissue: whole kidneys agent: vehicle dose: control
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Treatment protocol |
Cisplatin (0.5 mg/mL; Randa®; Nippon Kayaku Co. Ltd., Tokyo, Japan) was administered intraperitoneally; the same volume of saline solution was injected in the sham controls.
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Growth protocol |
Male Wistar/ST rats (8 weeks old) were purchased from SLC Animal Research Laboratories (Shizuoka, Japan). The rats were fed normal pellet food ad libitum, and given water freely.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with the RNeasy Mini Kit (Qiagen GmbH, Hilden, Germany)
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Label |
Digoxigenin-UTP
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Label protocol |
Digoxigenin (DIG)-labeled cRNA was generated from total RNA extracted from the proximal tubules or whole kidney using Applied Biosystems Chemiluminescent Reverse Transcription-In Vitro Transcription (RT-IVT) Labeling Kit v2.0 (Applied Biosystems part of Life Technologies, Foster City, CA, USA)
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Hybridization protocol |
A total of 10 μg of labeled cRNA from each RT-IVT reaction was hybridized onto Rat Genome Survey Microarray at 55°C for 16 h.
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Scan protocol |
Microarrays were analyzed using the ABI 1700 Chemiluminescent Microarray Analyzer (Applied Biosystems)
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Description |
Rat Genome Survey Microarray® (Applied Biosystems)
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Data processing |
The array contained 34,656 oligonucleotide probes, including 26,857 individual gene probes and more than 1000 control probes. Microarray signal data were analyzed using Spotfire® software (TIBCO Software Inc. Palo Alto, CA). Expression values were normalized across the experiments with each 50% percentile values. Genes were defined as detectable on the basis of the recommendations from Applied Biosystems; a gene with a signal:noise ratio > 3.0 in 2 or 3 assays, and a FLAG value < 5000 was considered to be detectable. Normalized signal data were analyzed statistically with a one-way ANOVA. Probability values of less than 0.05 were considered statistically significant.
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Submission date |
Apr 10, 2012 |
Last update date |
Dec 24, 2013 |
Contact name |
Satohiro Masuda |
E-mail(s) |
masuda@kuhp.kyoto-u.ac.jp
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Phone |
+81-75-751-3586
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Fax |
+81-75-751-3586
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Organization name |
Kyoto University Hospital
|
Department |
Pharmacy
|
Lab |
Masuda
|
Street address |
Shogoin
|
City |
Kyoto |
ZIP/Postal code |
606-8507 |
Country |
Japan |
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Platform ID |
GPL2996 |
Series (1) |
GSE37133 |
Gene expression profiles in isolated proximal tubules and whole kidneys of rats after cisplatin-administration |
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