|
| Status |
Public on Oct 04, 2012 |
| Title |
IRF6 shRNA-B |
| Sample type |
RNA |
| |
|
| Source name |
Adult Day5 ProEs
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: ex vivo differentiating ProEs
developmental stage: adult bone marrow
transfection: shRNAs against IRF6
|
| Treatment protocol |
CD34+ cells were transduced with shRNA lentiviruses, selected, and differentiated into proerythroblasts.
|
| Growth protocol |
Primary maturing adult erythroblasts were generated ex vivo using a serum-free two-phase liquid culture system.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Description |
Ex vivo erythroid differentiation of magnetically-sorted CD34+ HSPCs
|
| Data processing |
We RMA-normalized the arrays using the affy package from Bioconductor in R, and further eliminated batch effects using ComBat.
|
| |
|
| Submission date |
Apr 02, 2012 |
| Last update date |
Oct 04, 2012 |
| Contact name |
Jian Xu |
| E-mail(s) |
Jian.Xu@UTSouthwestern.edu
|
| Phone |
214-648-6125
|
| Organization name |
UT Southwestern Medical Center
|
| Department |
Children's Research Institute
|
| Street address |
6000 Harry Hines Blvd. NL12.138B
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75235 |
| Country |
USA |
| |
|
| Platform ID |
GPL15394 |
| Series (2) |
| GSE36988 |
Expression Profiling of Primary Human Proerythroblasts (ProEs) After IRF2, IRF6, and MYB shRNA Knockdown |
| GSE36994 |
Comparative profiling of human fetal and adult erythropoiesis |
|
