|
| Status |
Public on Feb 28, 2006 |
| Title |
MGAS315_OD=0.4_2a |
| Sample type |
RNA |
| |
|
| Source name |
Group A Streptococcus
|
| Organism |
Streptococcus sp. 'group A' |
| Characteristics |
Serotype M3 strain MGAS315
|
| Growth protocol |
Medium = Todd Hewit broth supplemented with 2% w/v yeast extract (THY broth)
Conditions = 37°C, atmosphere supplemented with 5% CO2, without aeration (statically)
Cells were harvested at an OD 600nm = 0.4, exponential phase growth
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction was performed as described by Graham et. al., American Journal of Pathology, 2005 166 #2 pg. 455-465
|
| Label |
biotin-ddUTP, fluorescent labeling was performed by the NIAID Affymetrix core facility
|
| Label protocol |
Labeling was performed as described by Graham et. al., American Journal of Pathology, 2005 166 #2 pg. 455-465
|
| |
|
| Hybridization protocol |
Hybridization was performed as described by Graham et. al., American Journal of Pathology, 2005 166 #2 pg. 455-465
|
| Scan protocol |
Scanning was performed as described by Graham et. al., American Journal of Pathology, 2005 166 #2 pg. 455-465
|
| Description |
Hybridization data from custom Affymetrix chip was masked to include only probe sets designed relative to the GAS metagenome sequence data
|
| Data processing |
Data were normalized using dchip 2005 software using the PM-MM model-based expression index
|
| |
|
| Submission date |
Jan 03, 2006 |
| Last update date |
Feb 27, 2006 |
| Contact name |
Stephen B. Beres |
| E-mail(s) |
sbberes@houstonmethodist.org
|
| Phone |
(713) 441-5067
|
| Organization name |
Houston Methodist Research Institute
|
| Department |
Pathology and Genomic Medicine
|
| Lab |
Infectious Disease Research
|
| Street address |
6670 Bertner Ave.
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL2129 |
| Series (1) |
| GSE3966 |
Expression comparison of GAS M3 strains MGAS315 & MGAS9887 in exponential growth |
|
