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GEO accession:
Sample GSM88168
Query DataSets for GSM88168
Status
Public on Aug 22, 2007
Title
yeast_nup84 null vs WT_glucose_rep4
Sample type
RNA
Channel 1
Source name
wild type, 2% glucose
Organism
Saccharomyces cerevisiae
Characteristics
wild type reporter strain (NJS2) from S288C background; grown to early-mid log in YPD at 23 degrees Celcius
Extracted molecule
total RNA
Label
Cy5
Channel 2
Source name
nup84 null, 2% glucose
Organism
Saccharomyces cerevisiae
Characteristics
nup84 null reporter strain (NJS10) from S288C background; grown to early-mid log in YEPD at 23 degrees Celcius
Extracted molecule
total RNA
Label
Cy3
Description
labeling done with Agilent's Low RNA Input Linear Amp kit; hybridization and slide processing done according to Agilent instructions
Data processing
log2 ratio after ratio-based and LOWESS normalization in Acuity 4.0
Submission date
Dec 16, 2005
Last update date
Aug 23, 2006
Contact name
Kellie Barbara
E-mail(s)
kellie.barbara@gmail.com
Organization name
Univ. of Southern MS
Department
Biological Sciences
Lab
Santangelo Lab
Street address
118 College Dr. #5018
City
Hattiesburg
State/province
MS
ZIP/Postal code
39406
Country
USA
Platform ID
GPL2883
Series (1)
GSE3853
Transcriptome response to the deletion of Gcr1, Nup84 or Nup84 & Gcr1 (double deletion)
Data table header descriptions
ID_REF
VALUE
ratio-based and LOWESS normalized log2 ratio
F635 Mean - B635
channel 1 mean minus background
F532 Mean - B532
channel 2 mean minus background
Data table
ID_REF
VALUE
F635 Mean - B635
F532 Mean - B532
1
-8.302
7
4159
2
-2.124
30
29
3
0.556
79
83
4
0.387
54
63
5
0.312
200
232
6
-0.272
124
68
7
-0.124
28
45
8
-0.407
39
73
9
0.771
203
183
10
0.955
345
273
11
-0.113
104
179
12
0.634
349
341
13
-0.709
14
33
14
-0.499
1554
3475
15
-1.525
7
23
16
-0.041
377
498
17
-0.097
181
117
18
1.495
1286
1384
19
1.661
1016
492
20
-0.846
10
22
Total number of rows:
10807
Table truncated, full table size
193 Kbytes
.
Supplementary data files not provided
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