|
| Status |
Public on Nov 02, 2012 |
| Title |
shG9a dex 24h 4859453023_F |
| Sample type |
RNA |
| |
|
| Source name |
shG9a dex 24h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
treatment: 100nM dexamethasone
time: 24h
knockdown: shG9a
|
| Treatment protocol |
Cells were treated with 100nM dexamethasone for 24 hours
|
| Growth protocol |
Cells were maintained in DMEM and hormone treated cells were plated and grown in phenol red-free medium supplemented with 5% charcoal stripped serum for 3 days prior to hormone treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the TRIzol method as per the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Labeling, hybridization and scanning was performed by Epigenome Center Core Facility at USC as per their standard protocol.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
shG9a_24h
4859453023_F
|
| Data processing |
Spatial artifacts removed with BASH, data normalized using neqc with limma in R
|
| |
|
| Submission date |
Feb 21, 2012 |
| Last update date |
Nov 02, 2012 |
| Contact name |
Dai-Ying Wu |
| Organization name |
University of Southern California
|
| Department |
Biochemistry
|
| Lab |
Stallcup
|
| Street address |
1441 Eastlake Ave, NOR 6314
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE35962 |
Promoter context-specific positive and negative transcriptional regulation of Glucocorticoid Receptor target genes by coregulator G9a |
|
