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Sample GSM859593 Query DataSets for GSM859593
Status Public on Feb 22, 2012
Title H3K27me3_1'AML_Ezh2-null
Sample type SRA
Source name leukemic spleen cells, primary, Ezh2-null, H3K27me3 ChIP
Organism Mus musculus
Characteristics background strain: C57BL/6
disease state: MLL-AF9-induced acute myeloid leukemia (AML)
cell type: leukemic spleen cells
cell origin: Ezh2-null MLL-AF9 leukemic cell from 1' recipient, sorted for GFP and YFP expression
chip antibody: H3K27me3
Treatment protocol GFP/YFP double-positive cells were isolated by flow sorting from spleens of leukemic mice.
Cells were subjected to ChIP using an antibody specific for H3K27me3.
Extracted molecule genomic DNA
Extraction protocol ChIP DNA libraries were made following the Illumina ChIP-seq library preparation kit.
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2000
Description H3K27me3_1'AML_Ezh2-null
Chromatin IP against H3K27me3.
Data processing Sequence reads were aligned to mouse genome assembly mm8 using Bowtie. The ChIP-Seq signal was quantified as total number of reads per million in the region of interest. An empirical background distribution model of reads was constructed to find the significance level of signal in a given region.
Submission date Jan 09, 2012
Last update date May 15, 2019
Contact name Amit Sinha
Phone 617-582-7579
Organization name Dana-Farber Cancer Institute
Department Pediatric Oncology
Lab Armstrong Lab
Street address 44 Binney St
City Boston
State/province MA
ZIP/Postal code 02135
Country USA
Platform ID GPL13112
Series (2)
GSE34962 Epigenetic profiling of WT and Ezh2-null MLL-AF9 murine leukemic cells
GSE34963 The Polycomb Repressive Complex 2 Is Required For MLL-AF9 Leukemia
SRA SRX115550
BioSample SAMN00771668
Named Annotation GSM859593_EZH2_KO.H3K27me3.batch2.n_0_l_28_e_70_a_m_1_best_strata.mm8.wig.gz

Supplementary file Size Download File type/resource
GSM859593_EZH2_KO.H3K27me3.batch2.n_0_l_28_e_70_a_m_1_best_strata.mm8.wig.gz 65.7 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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