|
| Status |
Public on Jul 04, 2012 |
| Title |
iPS2-a |
| Sample type |
RNA |
| |
|
| Source name |
iPSCs line 2 from HFF1, un-treated
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: iPS2-a derived from HFF1
treatment: none
passage: P15
tissue: undifferentiated iPSCs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNA was isolated using the RNeasy Mini Kit (Qiagen), including RNase-free Dnase (Qiagen) digestion (RNase-free DNase Set, Quiagen) has been applied on the columns during isolation.
|
| Label |
biotin
|
| Label protocol |
Illumina Gene Expression protocols contain a first- and second-strand reverse transcription step, followed by a single in vitro transcription (IVT) amplification that incorporates biotin-labeled nucleotides. Subsequent steps include array hybridization, washing, blocking, and streptavadin-Cy3 staining. Fluorescence emission by Cy3 is quantitatively detected for downstream analysis.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
|
| Data processing |
The data were normalised using rank invariant normalisation after background subtraction using the Gene Expression Module 1.8.0 of GenomeStudio V2010.3 (Illumina).
|
| |
|
| Submission date |
Jan 09, 2012 |
| Last update date |
Jul 04, 2012 |
| Contact name |
Katharina Drews |
| Organization name |
Max Planck Institute for Molecular Genetics
|
| Department |
Vertebrate Genomics
|
| Lab |
Molecular Embryology and Aging Group
|
| Street address |
Ihnestr. 63-73
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE34943 |
Transcriptome analysis of immediate early events in mRNA-mediated cellular reprogramming of human foreskin fibroblasts |
|
