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| Status |
Public on Jul 04, 2012 |
| Title |
Transcriptome analysis of immediate early events in mRNA-mediated cellular reprogramming of human foreskin fibroblasts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In this study human neonatal foreskin fibroblasts (HFF1 and BJ) were lipofected with an equal mixture of human reprogramming factor-encoding mRNAs. The cells were lysed and total RNA was harvested 24 hours post-transfection. Gene regulation was evaluated with respect to corresponding mock-transfected, Lipofectamine-treated control fibroblasts. For comparison, wildtype (un-treated) HFF1 and BJ fibroblasts as well as undifferentiated HFF1- and BJ-derived induced pluripotent stem cells and human embryonic stem cells (generated and maintained in our laboratory (refer to GSE26575) were included in the analysis.
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| Overall design |
Total RNA obtained from untreated human neonatal foreskin fibroblasts, reprogramming factor- and mock(Lipofectamine-treated)-transfected human neonatal fibroblasts, undifferentiated hESCs and iPSCs (derived from human neonatal foreskin fibroblasts).
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| Contributor(s) |
Adjaye J, Drews K |
| Citation(s) |
22381475 |
| Submission date |
Jan 09, 2012 |
| Last update date |
Mar 20, 2017 |
| Contact name |
Katharina Drews |
| Organization name |
Max Planck Institute for Molecular Genetics
|
| Department |
Vertebrate Genomics
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| Lab |
Molecular Embryology and Aging Group
|
| Street address |
Ihnestr. 63-73
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| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
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| Platforms (1) |
| GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA150973 |
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