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Sample GSM854225 Query DataSets for GSM854225
Status Public on Aug 06, 2012
Title C57BL/6 Brain_Sample 2_Non-IP Control
Sample type SRA
Source name whole brain
Organism Mus musculus
Characteristics strain: C57BL/6
age: Adult
sample type: no IP (input RNA)
rna subtype: RiboMinus RNA
library strategy: MeRIP-Seq
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from adult mouse brain and subjected to RiboMinus treatment to remove rRNAs. RNA was then subjected to 2 rounds of m6A RNA immunoprecipitation (MeRIP) using an antibody that recognizes m6A
Library strategy OTHER
Library source transcriptomic
Library selection other
Instrument model Illumina HiSeq 2000
Description no IP (input RNA)
Data processing Reads aligned to genome using BWA with default settings, converted to BAM files using SamTools, converted to sorted BAM files using SamTools, converted to bedgraphs using genomeCoverageBed and converted to bigwigs using bedGraphToBigWig
Peaks were found using MeRIPPeR software, inputting BWA-aligned reads, processed across 3 replicates for each organism.
Genome_build: mm9, hg19
Supplementary_files_format_and_content: bigwigs show number of reads mapping to the genome
Submission date Dec 27, 2011
Last update date May 15, 2019
Contact name Christopher E Mason
Organization name Weill Cornell Medical College
Department Physiology and Biophysics
Lab Christopher Mason Lab
Street address 1305 York Ave
City New York
ZIP/Postal code 10065
Country USA
Platform ID GPL13112
Series (1)
GSE29714 Comprehensive Analysis of mRNA Methylation Reveals Enrichment in 3' UTRs and Near Stop Codons
SRA SRX113244
BioSample SAMN00768932

Supplementary file Size Download File type/resource 79.6 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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